Abstract
Background: Diabetes mellitus is the most common disease in the world. One of the approaches therapy used to treat diabetes is inhibition of α -glucosidase activity by natural inhibitors from the plant.
Objective: Our work comes to highlight, in vitro, the effect of phenolic extracts of some Algerian Saharan Atlas medicinal plants on the activity of α-glucosidase.
Methods: The ethyl acetate extracts were prepared from hydro-methanolic (80:20) extracts of studied plants. The Folin-Ciocalteu method was used to determine the total phenolic content of the extracts. The α-glucosidase activity was determined by a microplate spectrophotometer at 405 nm using paranitrophenol α-D-glucopyranose as substrate.
Results: Among the plants tested Arthrophytum scoparium, Helianthemum lippii, Equisetum arvense and Cistus villosus exhibited a strong α-glucosidase inhibitory activity. According to IC50 values, Arthrophytum scoparium exerted the highest inhibitory activity against α-glucosidase (IC50= 5.02 µM) while a weak inhibitory activity was observed with Equisetum arvense (IC50 =21.88 µM). The kinetic study revealed a noncompetitive type inhibition for Arthrophytum scoparium (KI = 4.16 µM) and Helianthemum lippii (KI = 1.46 µM) extracts whereas a full mixed type inhibition (KI = 18.17 µM; KIS = 3.98 µM) and competitive type inhibition (KI = 25 µM) were observed for Cistus villosus and Equisetum arvense, respectively.
Conclusion: The results suggest that these plants represent a potential source to develop new antidiabetic drugs for controlling postprandial blood sugar.
Keywords: Atlas algerian plants, diabetes mellitus, inhibition, kinetic, phenolic extracts, α-glucosidase.
Graphical Abstract
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