Abstract
Background: Evidence is increasing that microRNAs (miR) are particularly important in lung homeostasis and development and have been shown to be involved in many pulmonary diseases such as asthma, chronic obstructive pulmonary disease, sarcoidosis, Lung Cancer (LC) and other smoking-related diseases.
Objective: The objective of this study was to investigate the expression of miR-155 and miR-486-5p in tissues from LC patients and healthy endobronchial mucosa as prognostic biomarkers for diagnosing LC.
Methods: Bronchoscopic and thoracoscopic tissue biopsies were taken from 50 LC patients and other 50 control subjects without lung mass, who were planned for a clinical bronchoscopy. The expressions of miR-155 and miR-486-5p in both tumor tissue and healthy mucosa were detected by quantitative real-time polymerase chain reaction.
Results: Histopathology showed that 72% of LC patients were in advanced stages III and IV, with non-small cell lung carcinoma and adenocarcinoma being the most common diagnosis. miR-155 was significantly overexpressed while, miR-486-5p was underexpressed, in LC patients as compared to controls. Area under receiver operating characteristic curves of miR-155 (<-0.9) and miR-486 (>-0.62) had sensitivity of 92 and 96% and specificity of 80 and 84%, respectively, in discriminating LC patients from controls with benign solitary pulmonary nodules.
Conclusion: miR-155 was highly overexpressed, yet it did not correlate with stages, while miR-486- 5p was extremely underexpressed and significantly correlated with stages of LC. Thus, their detection represents an excellent diagnostic/prognostic tool to support more established techniques linked to LC spread locally and systemically.
Keywords: Early diagnosis, lung cancer prognosis, microRNA, quantitative real-time polymerase chain reaction, smoking, biopsy.
Graphical Abstract
MicroRNA
Title:Underexpression of miR-486-5p but not Overexpression of miR-155 is Associated with Lung Cancer Stages
Volume: 7 Issue: 2
Author(s): Marwa A. Mohamed , Ehab I. Mohamed *, Samir A. Abd El-Kaream , Mohamed I. Badawi and Samy H. Darwish
Affiliation:
- Medical Biophysics, Medical Research Institute, Alexandria University, Alexandria,Egypt
Keywords: Early diagnosis, lung cancer prognosis, microRNA, quantitative real-time polymerase chain reaction, smoking, biopsy.
Abstract: Background: Evidence is increasing that microRNAs (miR) are particularly important in lung homeostasis and development and have been shown to be involved in many pulmonary diseases such as asthma, chronic obstructive pulmonary disease, sarcoidosis, Lung Cancer (LC) and other smoking-related diseases.
Objective: The objective of this study was to investigate the expression of miR-155 and miR-486-5p in tissues from LC patients and healthy endobronchial mucosa as prognostic biomarkers for diagnosing LC.
Methods: Bronchoscopic and thoracoscopic tissue biopsies were taken from 50 LC patients and other 50 control subjects without lung mass, who were planned for a clinical bronchoscopy. The expressions of miR-155 and miR-486-5p in both tumor tissue and healthy mucosa were detected by quantitative real-time polymerase chain reaction.
Results: Histopathology showed that 72% of LC patients were in advanced stages III and IV, with non-small cell lung carcinoma and adenocarcinoma being the most common diagnosis. miR-155 was significantly overexpressed while, miR-486-5p was underexpressed, in LC patients as compared to controls. Area under receiver operating characteristic curves of miR-155 (<-0.9) and miR-486 (>-0.62) had sensitivity of 92 and 96% and specificity of 80 and 84%, respectively, in discriminating LC patients from controls with benign solitary pulmonary nodules.
Conclusion: miR-155 was highly overexpressed, yet it did not correlate with stages, while miR-486- 5p was extremely underexpressed and significantly correlated with stages of LC. Thus, their detection represents an excellent diagnostic/prognostic tool to support more established techniques linked to LC spread locally and systemically.
Export Options
About this article
Cite this article as:
Mohamed A. Marwa , Mohamed I. Ehab *, El-Kaream A. Abd Samir, Badawi I. Mohamed and Darwish H. Samy , Underexpression of miR-486-5p but not Overexpression of miR-155 is Associated with Lung Cancer Stages, MicroRNA 2018; 7 (2) . https://dx.doi.org/10.2174/2211536607666180212124532
DOI https://dx.doi.org/10.2174/2211536607666180212124532 |
Print ISSN 2211-5366 |
Publisher Name Bentham Science Publisher |
Online ISSN 2211-5374 |

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Molecular Biotheranostic Approaches of Cancers Using LAT Kit Probes
Current Medical Imaging Non-Small Cell Lung Carcinoma: An Overview on Targeted Therapy
Current Drug Targets Clinical Applications of the Urokinase Receptor (uPAR) for Cancer Patients
Current Pharmaceutical Design Role of the Retinoblastoma Tumor Suppressor in the Maintenance of Genome Integrity
Current Molecular Medicine Leukotriene A4 Hydrolase as a Target for Cancer Prevention and Therapy
Current Cancer Drug Targets Ubiquitylation and Cancer Development
Current Cancer Drug Targets Erythropoietin in Cancer: An Update
Current Molecular Medicine Nanoparticle-Delivered Quercetin for Cancer Therapy
Anti-Cancer Agents in Medicinal Chemistry Therapeutic Potential of Perineural Invasion, Hypoxia and Desmoplasia in Pancreatic Cancer
Current Pharmaceutical Design The IGF-I/IGF-I Receptor Pathway: Implications in the Pathophysiology of Thyroid Cancer
Current Medicinal Chemistry A Progressive Review of V600E-B-RAF-Dependent Melanoma and Drugs Inhibiting It
Mini-Reviews in Medicinal Chemistry A Comprehensive Review on Pharmacognostical Investigation and Pharmacology of Typhonium trilobatum
The Natural Products Journal An Overview on Different Classes of Viral Entry and Respiratory Syncitial Virus (RSV) Fusion Inhibitors
Current Medicinal Chemistry The Use of SIFT-MS to Investigate Headspace Aldehydes as Markers of Lipid Peroxidation
Current Analytical Chemistry SENP1 as A Biomarker for the Diagnosis of Cancer: Review of the Science and Published Patents
Recent Patents on Biomarkers Streamlined In Vivo Selection and Screening of Human Prostate Carcinoma Avid Phage Particles for Development of Peptide Based In Vivo Tumor Imaging Agents
Combinatorial Chemistry & High Throughput Screening Application of NMR Metabolomics to Search for Human Disease Biomarkers
Combinatorial Chemistry & High Throughput Screening Targeting of Leukemia-Initiating Cells to Develop Curative Drug Therapies: Straightforward but Nontrivial Concept
Current Cancer Drug Targets Intramolecular Cyclisation of β-Aryl-β-Amino Acids in the Design of Novel Heterocyclic Systems with Therapeutic Interest: An Unfailing Source of Diversity
Current Medicinal Chemistry Prediction and Early Evaluation of Anticancer Therapy Response: From Imaging of Drug Efflux Pumps to Targeted Therapy Response
Current Medicinal Chemistry