Antioxidant and Antinociceptive Activities of Methanol Extract of Muehlenbeckia sagittifolia

Title:Antioxidant and Antinociceptive Activities of Methanol Extract of Muehlenbeckia sagittifolia

Volume: 4 Issue: 1

Author(s): Karine Bertoldi, Felipe dos Santos Moyses, Mara Rejane Ritter, Elina Bastos Caramao, Maria Ines Melecchi and Ionara Rodrigues Siqueira*

Affiliation:

• Departamento de Farmacologia, Instituto de Ciencias Basicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500 sala 202, 90050-170, Porto Alegre, Rio Grande do Sul,Brazil

Keywords: Antioxidant effect, formalin test, in vitro techniques, mice, xanthine oxidase, methanol.

Abstract: Background: Muehlenbeckia sagittifolia (Ortega) Meisn. is widely used to manage arthritis, inflammation, hepatic disorders, skin conditions, allergies, and diabetes mellitus.

Objective: The aim of this work was to investigate the antioxidant properties as well antinociceptive activity of methanol extract of M. sagittifolia (MEMS).

Method: The antioxidant properties of methanol extract of MEMS were evaluated using different in vitro systems. A xanthine/xanthine oxidase system, which generates superoxide radicals, was used to evaluate the superoxide radical scavenging activity of MEMS and its ability to inhibit xanthine oxidase activity. The interaction with peroxyl radicals was assayed by the luminol chemiluminescence intensity induced by an azo initiator, with the measurements of total reactive antioxidant potential and total antioxidant reactivity. The hydrogen peroxide scavenging and metal chelating activities also were evaluated. In addition, antinociceptive activity of MEMS was investigated by formalin test.

Results: The GC-MS analysis of the extract showed a mixture of different compounds including hexadecanoic and octadecatrienoic acids as well as vitamin E. MEMS inhibited xanthine oxidase activity and showed the ability to scavenge peroxyl and superoxide radicals. However, MEMS did not show any metal chelating activity and was unable to scavenge hydrogen peroxide. MEMS (intraperitoneally) was ineffective in first phase of the formalin test what could exclude centrally mediated antinociceptive activity. MEMS significantly inhibited the late nociception phase induced by formalin in mice, indicating an anti-inflammatory activity.

Conclusion: Our results suggest that Muehlenbeckia sagittifolia exhibits antioxidant activity and antinociceptive action in inflammatory pain and can validate its traditional use in inflammatory conditions.

Cite this article as:

Bertoldi Karine , dos Santos Moyses Felipe , Rejane Ritter Mara , Bastos Caramao Elina , Ines Melecchi Maria and Rodrigues Siqueira Ionara *, Antioxidant and Antinociceptive Activities of Methanol Extract of Muehlenbeckia sagittifolia, Current Traditional Medicine 2018; 4 (1) . https://dx.doi.org/10.2174/2215083803666171124160149