Bioinformatics Approaches to Profile the Tumor Microenvironment for Immunotherapeutic Discovery

Title:Bioinformatics Approaches to Profile the Tumor Microenvironment for Immunotherapeutic Discovery

Volume: 23 Issue: 32

Author(s): Trevor Clancy, Ruth Dannenfelser, Olga Troyanskaya, Karl Johan Malmberg, Eivind Hovig and Vessela Kristensen*

Affiliation:

• Department of Clinical Molecular Biology (EpiGen), Division of Medicine, Akershus University Hospital, Lørenskog, and University of Oslo, Oslo,Norway

Keywords: Bioinformatics, Systems-immunology, Immunotherapy, gene expression profiling, immune-cell infiltration, Immuno-oncology.

Abstract: In the microenvironment of a malignancy, tumor cells do not exist in isolation, but rather in a diverse ecosystem consisting not only of heterogeneous tumor-cell clones, but also normal cell types such as fibroblasts, vasculature, and an extensive pool of immune cells at numerous possible stages of activation and differentiation. This results in a complex interplay of diverse cellular signaling systems, where the immune cell component is now established to influence cancer progression and therapeutic response. It is experimentally difficult and laborious to comprehensively and systematically profile these distinct cell types from heterogeneous tumor samples in order to capitalize on potential therapeutic and biomarker discoveries. One emerging solution to address this challenge is to computationally extract cell-type specific information directly from bulk tumors. Such in silico approaches are advantageous because they can capture both the cell-type specific profiles and the tissue systems level of cell-cell interactions. Accurately and comprehensively predicting these patterns in tumors is an important challenge to overcome, not least given the success of immunotherapeutic drug treatment of several human cancers. This is especially challenging for subsets of closely related immune cell phenotypes with relatively small gene expression differences, which have critical functional distinctions. Here, we outline the existing and emerging novel bioinformatics strategies that can be used to profile the tumor immune landscape.

Cite this article as:

Clancy Trevor , Dannenfelser Ruth, Troyanskaya Olga, Malmberg Johan Karl, Hovig Eivind and Kristensen Vessela *, Bioinformatics Approaches to Profile the Tumor Microenvironment for Immunotherapeutic Discovery, Current Pharmaceutical Design 2017; 23 (32) . https://dx.doi.org/10.2174/1381612823666170710154936