摘要
目的:本研究旨在研究淀粉样蛋白(Aβ)诱导人视网膜色素上皮细胞系(ARPE-19)细胞产生血管生成因子的机制。 方法:本研究采用美国典型培养物保藏中心(ATCC)获得的ARPE-19细胞。 通过实时定量PCR(RT-PCR)测量血管内皮生长因子(VEGF),白细胞介素-8(IL-8),单核细胞趋化蛋白-1(MCP-1)和补体活化片段C3a和C5a的表达水平和酶联免疫吸附测定(ELISA)。运用流式细胞术测量线粒体相关活性氧(ROS)的产生。 结果:Aβ治疗ARPE-19细胞中VEGF,IL-8,MCP-1,C3a和C5a的表达显着增加。线粒体相关的ROS当暴露于Aβ时,其产生也显着增加。用二苯基碘化铵(DPI)抑制线粒体ROS显着降低了ARPE-19细胞对Aβ诱导的VEGF,IL-8,MCP-1,C3a和C5a的产生。抗AβC3a或抗-C5a中和抗体在用Aβ刺激时对ARPE-19细胞的VEGF,IL-8和MCP-1的分泌没有可检测的影响。 结论:我们的结果支持Aβ通过RPE细胞促进血管生成细胞因子VEGF,IL-8和MCP-1的产生参与脉络膜新血管形成(CNV)发病机制的假说。显示线粒体ROS在调节Aβ诱导的这些细胞因子的表达中起作用。
关键词: 淀粉样蛋白β,玻璃疣,脉络膜新血管形成,视网膜色素上皮,年龄相关性黄斑变性,线粒体相关活性氧。
Current Molecular Medicine
Title:The Role of Mitochondria-Associated Reactive Oxygen Species in the Amyloid β Induced Production of Angiogenic Factors b y ARPE-19 Cells
Volume: 17 Issue: 2
关键词: 淀粉样蛋白β,玻璃疣,脉络膜新血管形成,视网膜色素上皮,年龄相关性黄斑变性,线粒体相关活性氧。
摘要: Objective: This study aimed to investigate the mechanisms whereby Amyloidbeta (Aβ) induces the production of angiogenic factors by a human retinal pigment epithelial cell line (ARPE-19) cells.
Methods: ARPE-19 cells obtained from the American Type Culture Collection (ATCC) were utilized in this study. The expression level of vascular endothelial growth factor (VEGF), Interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1) and complement activation fragments C3a and C5a were measured by Real-time quantitative PCR (RT-PCR) and Enzyme-linked immunosorbent assay (ELISA). The production of mitochondria-associated reactive oxygen species (ROS) was measured by flow cytometry. Results: The expression of VEGF, IL-8, MCP-1, C3a and C5a was significantly increased in Aβ-treated ARPE-19 cells. Mitochondria-associated ROS production was also significantly increased when exposed to Aβ. Inhibition of mitochondrial ROS with Diphenyleneiodonium chloride (DPI) markedly decreased the Aβ induced production of VEGF, IL-8, MCP-1, C3a and C5a by ARPE-19 cells. Anti-C3a or anti-C5a neutralizing antibodies did not have a detectable influence on the secretion of VEGF, IL-8 and MCP-1 by ARPE-19 cells upon stimulation with Aβ. Conclusion: Our results support the hypothesis that Aβ is involved in the pathogenesis of choroidal neovascularization (CNV) formation by promoting the production of the angiogenic cytokines VEGF, IL-8 and MCP-1 by RPE cells. Mitochondrial ROS was shown to play a role in the regulation of Aβ induced expression of these cytokines.Export Options
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Cite this article as:
The Role of Mitochondria-Associated Reactive Oxygen Species in the Amyloid β Induced Production of Angiogenic Factors b y ARPE-19 Cells, Current Molecular Medicine 2017; 17 (2) . https://dx.doi.org/10.2174/1566524017666170331162616
DOI https://dx.doi.org/10.2174/1566524017666170331162616 |
Print ISSN 1566-5240 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5666 |
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