Inhibition of Aβ(1-42)Oligomerization, Fibrillization and Acetylcholinesterase Activity by Some Anti-Inflammatory Drugs: An in vitro Study

Title:Inhibition of Aβ(1-42)Oligomerization, Fibrillization and Acetylcholinesterase Activity by Some Anti-Inflammatory Drugs: An in vitro Study

Volume: 15 Issue: 3

Author(s): Hamendra S. Parmar*, Anshul Assaiya, Renuka Agrawal, Shalini Tiwari, Iram Mufti, Neha Jain, Elangovan Manivannan, Tushar Banerjee and Anil Kumar

Affiliation:

• School of Biotechnology, Devi Ahilya University, Takshashila Campus, Khandwa Road, Indore-452001, M.P.,India

Keywords: Acetylcholinesterase activity, anti-inflammatory drugs, Aβ oligomerization, fibrillization, nitric oxide radicals, singlet oxygen.

Abstract: Background: Number of contradictory reports are available on the effects of antiinflammatory drugs on Alzheimer’s disease (AD) including beneficial, adverse and stage dependent effects. We provide insights of the effects exerted by some anti-inflammatory drugs on the chemistry of AD.

Methods: Three different doses of dexamethasone (0.015, 0.030, 0.060 μM), piroxicam (5, 7.5, 10 μM), indomethacin (1, 1.25, 1.50 μM), diclofenac (0.6, 0.8, 1.0 μM), aspirin (90, 120, 150 μM) and celecoxib (30, 45, 60 μM) were used. Rivastigmine, methylene blue and butylated hydroxyanisole were used as standard drug, oligomerization inhibitor and antioxidant, respectively. Oligomerization and fibrillization reactions were performed using Aβ1-42 peptides. Results-Indomethacin and aspirin mainly inhibited oligomerization, while rivastigmine and piroxicam inhibited fibrillization. Diclofenac and celecoxib inhibited both oligomerization and fibrillization almost equally. Dexamethasone showed poor efficiency on both the processes, but exert comparably more inhibition of oligomerization than fibrillization. Inhibition of acetylcholinesterase activity was also potent and was in the following order: celecoxib> piroxicam> diclofenac> aspirin> indomethacin> dexamethasone. Strong radical scavenging (More than 50%) activity was showed by indomethacin and aspirin for NO radicals.

Conclusion: Present study consistently revealed that anti-inflammatory drugs have potential to Modulate chemistry of AD progression. Inclusion of anti-inflammatory drugs in low doses along with routine therapies may provide therapeutically and economically more efficient therapies for AD. However, further studies are warranted, because the overall therapeutic effect seems to be the function of stage of disease, dose of drug, main underlying mechanism of action(s).

Cite this article as:

Parmar S. Hamendra*, Assaiya Anshul, Agrawal Renuka, Tiwari Shalini, Mufti Iram, Jain Neha, Manivannan Elangovan, Banerjee Tushar and Kumar Anil, Inhibition of Aβ(1-42)Oligomerization, Fibrillization and Acetylcholinesterase Activity by Some Anti-Inflammatory Drugs: An in vitro Study, Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry 2016; 15 (3) . https://dx.doi.org/10.2174/1871523015666161229143936

DOI https://dx.doi.org/10.2174/1871523015666161229143936	Print ISSN 1871-5230
Publisher Name Bentham Science Publisher	Online ISSN 1875-614X