Central Composite Designed Imatinib-Loaded Magnetic Nanoparticles

Title:Central Composite Designed Imatinib-Loaded Magnetic Nanoparticles

Volume: 6 Issue: 2

Author(s): Mandeep Dahiya and Harish Dureja

Affiliation:

Keywords: Central composite design, chitosan, imatinib, ionic gelation method, magnetic nanoparticles.

Abstract: Background: Treatment by using magnetic nanoparticles is an emerging concept. Currently, magnetic nanoparticles (MNP) are being used for hyperthermia, targeting of drugs (active or passive), and for diagnostic purposes using nuclear magnetic resonance imaging.

Objective: In the present study, imatinib-loaded chitosan magnetic nanoparticles were formulated by modified ionic gelation method. The purpose of the study was to explore the effect of the concentration of magnetite (X1) and the concentration of chitosan (X2) on the encapsulation of imatinib from magnetic nanoparticles using a central composite design.

Methods: The formulated magnetic nanoparticles were characterized by laser light scattering, Fourier transform infrared spectroscopy, scanning electron microscope, and zeta potential measurement.

Results: It was found that batch MNP-4 has the maximum encapsulation efficiency, loading capacity, and minimum particle size achieved for the optimized batch. Scanning Electron Microscopy (SEM) results confirmed a sphere-shaped or ellipsoidal morphology of the nanoparticles. A particle size analysis gives an average diameter of 188 nm. The encapsulation efficiency and drug loading were found to be (7.22 ± 0.13) and (5.16 ± 0.34) mg/100 mg, respectively, for the optimized batch. In-vitro drug release studies disclosed that 91.05% of the drug was released cumulatively. The magnetic characteristics were confirmed by a vibrating sample magnetometer. The saturation of magnetization was found to be 1.408 emu/g. VSM analysis confirmed that the as-prepared magnetic chitosan nanoparticles have a satisfactory magnetic receptivity for a prospective magnetic drug carrier for targeted delivery. The encapsulation efficiency was highest at the highest levels of chitosan and magnetite concentration. The encapsulation was lowest at the lowest level of the chitosan concentration.

Conclusion: The magnetic and nano-effects due to the presence of magnetite and crosslinked chitosan are strong determining factors for the utilization of the prepared nanoparticles for various targeted applications. The model developed in the current study can be further utilized as response surface for the encapsulation efficiency of MNPs.

Cite this article as:

Dahiya Mandeep and Dureja Harish, Central Composite Designed Imatinib-Loaded Magnetic Nanoparticles, Current Nanomedicine 2016; 6 (2) . https://dx.doi.org/10.2174/2468187306666160802125718