Optimization of the Enzymolysis Conditions for Scorpion Peptides and Evaluation of its Antitumor Activity

Title:Optimization of the Enzymolysis Conditions for Scorpion Peptides and Evaluation of its Antitumor Activity

Volume: 11 Issue: 1

Author(s): Fangwen Jiao, Jihui Wang, Yuhong Liu, Yan Zou, Wei Jia, Panpan Zhang, Jing Sun, Youwei Xu and Junxiang Wang

Affiliation:

Keywords: Orthogonal design experiment, enzymolysis conditions, anti-tumor activity, cell apoptosis, cell cycle analysis.

Abstract: Trypsin, chymotrypsin and elastase were combined to hydrolyze scorpion Buthus marstesii Karsch powders to prepare scorpion peptides (SP). Orthogonal design experiment L₁₆ (4⁴) was used to optimize variables of enzymolysis temperature, time, pH and enzyme concentration. Degree of hydrolysis (DH) and inhibition rate (IR) of A549 cells were selected as analysis indicators. The optimum enzymolysis conditions were determined as follows: enzymolysis temperature 40°C, time 3h, pH 8.5 and enzyme concentration of each 2000U. The anti-tumor activity of SP was determined on a panel of representative cell lines (A549, MCF7 and EC109) using MTT assay. In vitro, SP significantly inhibited the proliferation of A549, which was more obvious than MCF-7 and EC109. Further experiments on cell apoptosis and cell cycle analysis revealed that SP induce apoptosis and arrest cell cycle progression in S phase.

Cite this article as:

Jiao Fangwen, Wang Jihui, Liu Yuhong, Zou Yan, Jia Wei, Zhang Panpan, Sun Jing, Xu Youwei and Wang Junxiang, Optimization of the Enzymolysis Conditions for Scorpion Peptides and Evaluation of its Antitumor Activity, Current Signal Transduction Therapy 2016; 11 (1) . https://dx.doi.org/10.2174/1574362411666160517113916