Abstract
Introduction: In present study, a simple liquid-liquid extraction-high performance liquid chromatography–fluorescence method was developed for the determination of fluoxetine (FLU) in rat plasma samples.
Materials and Methods: A MZ Analytical Column C18 (250 mm × 4.6 mm, 5µm i.d.) was used for the separation of FLU. The total analysis time was less than 7 minutes. The rat plasma samples were simply treated with acetonitrile and analyzed using developed method.
Conclusion: The recovery of the FLU from rat plasma samples is 92.70% ± 0.48. FLU in spiked rat plasma samples provided linear data over a range of 0.2 – 2.00 µg mL-1. The method was validated according to the FDA guidelines and can be proposed for quantification of FLU in rat plasma.
Keywords: Fluoxetine, high performance liquid chromatography, rat plasma, validation, fluorescence detection.
Graphical Abstract
Related Journals
Related Books
New Findings from Natural Substances
Pharmaceutical Chemistry and Production: An Introductory Textbook
Evidence-Based Research in Ayurveda against COVID-19 in Compliance with Standardized Protocols and Practices
Pharmaceuticals for Targeting Coronaviruses
Medical Applications of Beta-Glucan
Nanotechnology Driven Herbal Medicine for Burns: From Concept to Application
Postbiotics: Science, Technology and Applications
Biologically Active Natural Products from Asia and Africa: A Selection of Topics
24
1