摘要
氧化应激和神经炎症与包括老年痴呆(AD)的多种神经退行性疾病的病理过程高度相关。(+)-2-(1-羟基-4-环氧己基)咖啡酸乙酯(HOEC),一种新5脂氧合酶抑制剂,是从Incarvillea mairei var. granditlora (Wehrhahn) Grierson全草中分离出的。我们研究了HOEC在体内和体外对抗过氧化氢(H2O2)和脂多糖(LPS)诱导的细胞毒性和神经炎症的保护作用。我们进行了MTT试验,LDH释放试验,形态学观察和Hoechst 33342/PI双染色结合EIA,免疫荧光染色和蛋白免疫印迹分析,以说明HOEC的神经保护作用。在H2O2暴露前的HOEC多浓度处理显著增强了细胞生存力,减少了LDH释放,防止了细胞的形态变化和凋亡。HOEC显著地抑制了LTB4的产生和巨噬细胞介导的神经毒性,而不是PGE2的减少。蛋白免疫印迹和免疫荧光染色显示HOEC抑制了H2O2诱导的p38磷酸化和NF-κB活化。HOEC的神经保护作用被p38抑制剂消除。我们发现了HOEC防止H2O2损害SH-SY5Y细胞和通过花生四烯酸网络调整的LPS诱导的损伤及其后的p38 MAPK和NF-κB信号。这些发现是我们考虑将HOEC作为预防和治疗涉及氧化应激或/和炎症的神经退行性疾病的候选药物。
关键词: 老年痴呆,HOEC,氧化应激,神经炎症,神经退行性疾病,MAPK,NF-κB。
Current Alzheimer Research
Title:Neuroprotection of (+)-2-(1-Hydroxyl-4-Oxocyclohexyl) Ethyl Caffeate Against Hydrogen Peroxide and Lipopolysaccharide Induced Injury via Modulating Arachidonic Acid Network and p38-MAPK Signaling
Volume: 12 Issue: 9
Author(s): Jiao-Ning Shen, Liu-Xin Xu, Lei Shan, Wei-Dong Zhang, Hong-Lin Li and Rui Wang
Affiliation:
关键词: 老年痴呆,HOEC,氧化应激,神经炎症,神经退行性疾病,MAPK,NF-κB。
摘要: Oxidative stress and neuroinflammation are highly relevant to the pathological processes of various neurodegenerative diseases including Alzheimer’s disease (AD). (+)-2-(1-hydroxyl-4-oxocyclohexyl) ethyl caffeate (HOEC), a novel 5-lipoxygenase inhibitor, was isolated from the whole plant of Incarvillea mairei var granditlora (Wehrhahn) Grierson. In this study, we investigated the protective effect of HOEC on hydrogen peroxide (H2O2) and lipopolysaccharide (LPS) -induced cytotoxicity and neuroinflammation in vitro and in vivo. MTT assay, LDH release assay, morphological observation and Hoechst 33342/PI dual staining followed by EIA, immunofluorescence staining and Western Blotting analysis were performed to elucidate the neuroprotective effect of HOEC. Treatment with HOEC at various concentrations prior to H2O2 exposure significantly enhanced cell viability, decreased LDH release, prevented cell morphologic changes and apoptosis. Instead of PGE2 reduction, HOEC markedly inhibited the production of LTB4 and suppressed the macrophage-mediated neurotoxicity. Western blotting and immunofluorescence staining showed that HOEC inhibited H2O2-induced p38 phosphorylation and NF-κB activation. Neuroprotective effect of HOEC was abolished by a p38 inhibitor. Further in vivo studies of LPS-induced neuroinflammation confirmed the anti-inflammatory effects of HOEC. These findings that HOEC protects SH-SY5Y cells from H2O2 and LPS-induced injury via arachidonic acid network modulation followed by p38 MAPK and NF-κB signaling, might make HOEC be considered as a therapeutic candidate for prevention and treatment of neurodegenerative diseases involving oxidative stress or/and inflammation.
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Jiao-Ning Shen, Liu-Xin Xu, Lei Shan, Wei-Dong Zhang, Hong-Lin Li and Rui Wang , Neuroprotection of (+)-2-(1-Hydroxyl-4-Oxocyclohexyl) Ethyl Caffeate Against Hydrogen Peroxide and Lipopolysaccharide Induced Injury via Modulating Arachidonic Acid Network and p38-MAPK Signaling, Current Alzheimer Research 2015; 12 (9) . https://dx.doi.org/10.2174/156720501209151019111244
DOI https://dx.doi.org/10.2174/156720501209151019111244 |
Print ISSN 1567-2050 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5828 |
Call for Papers in Thematic Issues
Current updates on the Role of Neuroinflammation in Neurodegenerative Disorders
Neuroinflammation is an invariable hallmark of chronic and acute neurodegenerative disorders and has long been considered a potential drug target for Alzheimer?s disease (AD) and dementia. Significant evidence of inflammatory processes as a feature of AD is provided by the presence of inflammatory markers in plasma, CSF and postmortem brain ...read more
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