摘要
背景:骨缺损会导致严重的衰弱和生活质量的下降。骨再生可以减轻骨缺损的一些并发症。在未来的治疗中,一个单一的facTor可以引起有效的骨再生是非常可取的,因为它将更有成本效益,任何非目标效应都将更容易被监控和潜在的管理,并且为了便于管理员。配给,这将导致更好的病人依从和满意。 目的:我们证明色素上皮衍生因子(PEDF)是一种抗血管生成的有效因子,在体外促进间充质干细胞成骨细胞的发生。,但不需要细胞共包封在藻酸盐珠支架中进行体内成骨。 结果:经免疫印迹和免疫细胞化学染色证实PEDF体外成骨分化为骨标记物(碱性磷酸酶、骨钙素、骨桥蛋白、Ⅰ型胶原)、钙蛋白(Ca)。酸化矿物沉积,碱性磷酸酶活性测定。微计算机体层摄影(Microct)、组织学(H)证实了PEDF介导的肌肉囊内骨形成。aematoxylin和eosin,AlcianBlue染色),免疫染色检测骨标记物和Ⅰ型胶原蛋白(热休克蛋白47和I型基质金属蛋白酶)。 结论:PEDF是一种很有前途的成骨生物。
关键词: PEDF,骨,干细胞,I型胶原,海藻酸钠,再生医学。
图形摘要
Current Drug Targets
Title:Alginate Bead-Encapsulated PEDF Induces Ectopic Bone Formation In Vivo in the Absence of Co-Administered Mesenchymal Stem Cells
Volume: 19 Issue: 5
关键词: PEDF,骨,干细胞,I型胶原,海藻酸钠,再生医学。
摘要: Background: Bone defects can be severely debilitating and reduce quality of life. Osteoregeneration can alleviate some of the complications in bony defects. For therapeutic use in future, a single factor that can cause potent bone regeneration is highly preferred as it will be more costeffective, any off-target effects will be more easily monitored and potentially managed, and for ease of administration which would lead to better patient compliance and satisfaction.
Objective: We demonstrate that pigment epithelium-derived factor (PEDF), one such factor that is known to be potent against angiogenesis, promotes osteoblastogenesis in mesenchymal stem cells in vitro, but does not need co-encapsulation of cells in alginate bead scaffolds for osteogeneration in vivo.
Results: Osteogenic differentiation by PEDF in vitro was confirmed with immunoblotting and immunocytochemical staining for bone markers (alkaline phosphatase, osteocalcin, osteopontin, collagen I), calcified mineral deposition, and assay for alkaline phosphatase activity. PEDF-mediated bone formation in a muscle pocket in vivo model was confirmed by microcomputed tomography (microCT), histology (haematoxylin and eosin, Alcian blue staining), immunostaining for bone markers and for collagen I-processing proteins (heat shock protein 47 and membrane type I matrix metalloproteinase).
Conclusion: PEDF therefore presents itself as a promising biological for osteogeneration.
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Cite this article as:
Alginate Bead-Encapsulated PEDF Induces Ectopic Bone Formation In Vivo in the Absence of Co-Administered Mesenchymal Stem Cells, Current Drug Targets 2018; 19 (5) . https://dx.doi.org/10.2174/1389450116666151001112455
DOI https://dx.doi.org/10.2174/1389450116666151001112455 |
Print ISSN 1389-4501 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-5592 |
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