A Facile, Microwave-Assisted Synthesis of an Adenosine-Ribose Probe for Binding-Based Profiling of Nucleoside and Nucleotide-Binding Proteins

Title:A Facile, Microwave-Assisted Synthesis of an Adenosine-Ribose Probe for Binding-Based Profiling of Nucleoside and Nucleotide-Binding Proteins

Volume: 3 Issue: 2

Author(s): Matthew R. Battistini, Shikha Mahajan, Daili Diaz, Lindsey N. Shaw and David J. Merkler

Affiliation:

Keywords: AdoRs, binding-based probe (BBP) binding-based protein profiling (BBPP), microwave-assisted synthesis.

Abstract: Herein, we describe a novel, microwave-assisted synthesis of a binding-based probe capable of enriching proteins that bind adenine ribose derivatives (AdoRs). We employed this probe in activity- based protein profiling studies to qualitatively assess the AdoR-binding proteome in three bacterial cell lines from the genus Bacillus. This proof of concept experiment demonstrated a unique subset of proteins distinctive to each species, and confirmed the efficacy of the probe tagging and subsequent enrichment.

Cite this article as:

Battistini R. Matthew, Mahajan Shikha, Diaz Daili, Shaw N. Lindsey and Merkler J. David, A Facile, Microwave-Assisted Synthesis of an Adenosine-Ribose Probe for Binding-Based Profiling of Nucleoside and Nucleotide-Binding Proteins, Current Microwave Chemistry 2016; 3 (2) . https://dx.doi.org/10.2174/2213335602666150706182206