Identification of L-Amino Acid Oxidase (Mb-LAAO) with Antibacterial Activity in the Venom of Montivipera bornmuelleri, a Viper from Lebanon

Title:Identification of L-Amino Acid Oxidase (Mb-LAAO) with Antibacterial Activity in the Venom of Montivipera bornmuelleri, a Viper from Lebanon

Volume: 13 Issue: 5

Author(s): Mohamad Rima, Claudine Accary, Katia Haddad, Riyad Sadek, Souad Hraoui-Bloquet, Jean C. Desfontis and Ziad Fajloun

Affiliation:

Keywords: Antibacterial effect, LAAO activity, L-amino acid oxidase, LC-ESI-MS analysis, Montivipera bornmuelleri.

Abstract: The L-amino acid oxidase (LAAO) is a multifunctional enzyme, able to partake in different activities including antibacterial activity. In this study, a novel LAAO (Mb-LAAO) was isolated from the venom of M. bornmuelleri snake using size exclusion chromatography followed by RP-HPLC and partially characterized. However, the molecular weight of the Mb-LAAO determined by ESI-MS and SDS-PAGE was 59 960.4 Da. Once the enzymatic activity test confirming the enzyme’s identity (transformation of L-leucine) was done, the Mb-LAAO was evaluated for its antibacterial activity against Gram-negative bacteria. It showed a remarkable effect against M. morganii and K. pneumoniae. Moreover, no cytotoxic activity was observed for Mb-LAAO against human erythrocytes arguing for an exploration of its pharmaceutical interest.

Cite this article as:

Rima Mohamad, Accary Claudine, Haddad Katia, Sadek Riyad, Hraoui-Bloquet Souad, Desfontis C. Jean and Fajloun Ziad, Identification of L-Amino Acid Oxidase (Mb-LAAO) with Antibacterial Activity in the Venom of Montivipera bornmuelleri, a Viper from Lebanon, Infectious Disorders - Drug Targets 2013; 13 (5) . https://dx.doi.org/10.2174/187152651305140403122334