Abstract
Over the past few years, the SNAP-tag technology has become a methodology with great potential in a variety of applications, e.g. the (specific) visualization of individual proteins and studies of protein interaction in living cells. Furthermore, the tag can be used for immunopurification and detection of recombinant proteins or site-specific coupling of recombinant proteins to surfaces. Next to the in vitro applications, it also enables detection of tagged proteins in vivo. This review gives an overview of the SNAP-tag technology in different fields of research and its potential for future developments.
Keywords: SNAP-tag, CLIP-tag, site-specific labeling, fluorescence microscopy, in-vivo imaging.
Current Pharmaceutical Design
Title:SNAP-Tag Technology: A General Introduction
Volume: 19 Issue: 30
Author(s): Katharina Kolberg, Christiane Puettmann, Alessa Pardo, Jenny Fitting and Stefan Barth
Affiliation:
Keywords: SNAP-tag, CLIP-tag, site-specific labeling, fluorescence microscopy, in-vivo imaging.
Abstract: Over the past few years, the SNAP-tag technology has become a methodology with great potential in a variety of applications, e.g. the (specific) visualization of individual proteins and studies of protein interaction in living cells. Furthermore, the tag can be used for immunopurification and detection of recombinant proteins or site-specific coupling of recombinant proteins to surfaces. Next to the in vitro applications, it also enables detection of tagged proteins in vivo. This review gives an overview of the SNAP-tag technology in different fields of research and its potential for future developments.
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Cite this article as:
Kolberg Katharina, Puettmann Christiane, Pardo Alessa, Fitting Jenny and Barth Stefan, SNAP-Tag Technology: A General Introduction, Current Pharmaceutical Design 2013; 19 (30) . https://dx.doi.org/10.2174/13816128113199990514
DOI https://dx.doi.org/10.2174/13816128113199990514 |
Print ISSN 1381-6128 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4286 |
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