Abstract
Introduction: The alterations in the expression of five different dopamine receptor genes (DRD1- DRD5) associated with certain diseases of the immune and nervous systems have previously been reported, and play multiple roles in various disorders such as cancer. This study focused on the relationship between changes in dopamine receptor gene expression and non - small - cell lung cancer.
Methods: Peripheral blood were obtained from 30 non-small cell lung cancer (NSCLC) patients and normal individuals. Total cellular RNA was extracted from peripheral blood mononuclear cells (PBMC) and cDNA was synthesized. Real - Time PCR was carried out using primer pairs specific for the five dopamine gene receptors mRNA and β-actin ( as internal control). Finally the specificities of the obtained PCR products for the respective dopamine receptors fragments were confirmed by sequencing. Results: Statistical analysis using SPSS software showed significant difference (P-value<0.05) in DRD2, DRD3 and DRD4 expression in NSCLC patients with comparison to normal individuals. The expression of these genes in the patient group was lower than those in the healthy controls, however, DRD1 and DRD5 genes did not show any significant changes.
Conclusions: It can be concluded that there is a quantitative significant difference of D2-like dopamine receptor genes expression in non- small -cell lung cancer that is not the same in all kind of receptor genes. On the other hand such significant differences and profiles could contribute to diagnosis, treatment, survey and monitoring of non- small- cell lung cancer. In addition, it could be of potential use in the design of future therapeutic strategies.
Keywords: Neurotransmitters, Dopamine receptors, Non-small cell lung cancer, Gene expression, Polymerase chain reaction
Current Cancer Therapy Reviews
Title:Significant Changes in D2-like Dopamine Gene Receptors Expression Associated with Non- Small -Cell Lung Cancer: Could it be of Potential Use in the Design of Future Therapeutic Strategies?
Volume: 8 Issue: 4
Author(s): Mojhgan Shaikhpoor, Ghasem Ahangari, Majid Sadeghizadeh, Adnan Khosravi and Gholamreza Derakhshani Deilami
Affiliation:
Keywords: Neurotransmitters, Dopamine receptors, Non-small cell lung cancer, Gene expression, Polymerase chain reaction
Abstract: Introduction: The alterations in the expression of five different dopamine receptor genes (DRD1- DRD5) associated with certain diseases of the immune and nervous systems have previously been reported, and play multiple roles in various disorders such as cancer. This study focused on the relationship between changes in dopamine receptor gene expression and non - small - cell lung cancer.
Methods: Peripheral blood were obtained from 30 non-small cell lung cancer (NSCLC) patients and normal individuals. Total cellular RNA was extracted from peripheral blood mononuclear cells (PBMC) and cDNA was synthesized. Real - Time PCR was carried out using primer pairs specific for the five dopamine gene receptors mRNA and β-actin ( as internal control). Finally the specificities of the obtained PCR products for the respective dopamine receptors fragments were confirmed by sequencing. Results: Statistical analysis using SPSS software showed significant difference (P-value<0.05) in DRD2, DRD3 and DRD4 expression in NSCLC patients with comparison to normal individuals. The expression of these genes in the patient group was lower than those in the healthy controls, however, DRD1 and DRD5 genes did not show any significant changes.
Conclusions: It can be concluded that there is a quantitative significant difference of D2-like dopamine receptor genes expression in non- small -cell lung cancer that is not the same in all kind of receptor genes. On the other hand such significant differences and profiles could contribute to diagnosis, treatment, survey and monitoring of non- small- cell lung cancer. In addition, it could be of potential use in the design of future therapeutic strategies.
Export Options
About this article
Cite this article as:
Shaikhpoor Mojhgan, Ahangari Ghasem, Sadeghizadeh Majid, Khosravi Adnan and Derakhshani Deilami Gholamreza, Significant Changes in D2-like Dopamine Gene Receptors Expression Associated with Non- Small -Cell Lung Cancer: Could it be of Potential Use in the Design of Future Therapeutic Strategies?, Current Cancer Therapy Reviews 2012; 8 (4) . https://dx.doi.org/10.2174/1573394711208040304
DOI https://dx.doi.org/10.2174/1573394711208040304 |
Print ISSN 1573-3947 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6301 |
Related Books
![](/images/wayfinder.jpg)
- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Therapeutic Perspectives on Pancreatic Cancer
Current Cancer Drug Targets Inhibition of Human Aromatase by Myosmine
Drug Metabolism Letters Bortezomib as the First Proteasome Inhibitor Anticancer Drug: Current Status and Future Perspectives
Current Cancer Drug Targets Stilbenes and Xanthones from Medicinal Plants as Potential Antitumor Agents
Current Bioactive Compounds MDA-7/IL-24-Based Cancer Gene Therapy: Translation from the Laboratory to the Clinic
Current Gene Therapy Beta-adrenergic Signaling in the Development and Progression of Pulmonary and Pancreatic Adenocarcinoma
Current Cancer Therapy Reviews Fiber-Optic Technologies in Laser-Based Therapeutics: Threads for a Cure
Current Pharmaceutical Biotechnology Cutaneous Melanoma: A Test Field for Immunotherapy and a Medical Challenge
Current Cancer Therapy Reviews Editorial [Hot topic: Contemporary Approaches in Cancer Therapy (Guest Editor: Riyaz Basha)]
Medicinal Chemistry HDAC Inhibitor Sodium Butyrate Augments the MEF2C Enhancement of Nampt Expression under Hypoxia
Current Pharmaceutical Design Triggering PIK3CA Mutations in PI3K/Akt/mTOR Axis: Exploration of Newer Inhibitors and Rational Preventive Strategies
Current Pharmaceutical Design Beneficial Effects of Bioactive Phospholipids: Genomic Bases
Current Nutrition & Food Science Targeting Antioxidants to Mitochondria: A Potential New Therapeutic Strategy for Cardiovascular Diseases
Current Pharmaceutical Design What We Learnt from Randomized Clinical Trials and Cohort Studies of Antioxidant Vitamin? : Focus on Vitamin E and Cardiovascular Disease
Current Pharmaceutical Biotechnology Nuclear Imaging of Prostate Cancer with Gastrin-Releasing-Peptide- Receptor Targeted Radiopharmaceuticals
Current Pharmaceutical Design Analysis of Drug Transport Kinetics in Multidrug-resistant Cells: Implications for Drug Action
Current Medicinal Chemistry Immunotherapeutic Approaches in Pancreatic Adenocarcinoma: Current Status and Future Perspectives
Current Molecular Pharmacology Molecular Bases of Liver Cancer Refractoriness to Pharmacological Treatment
Current Medicinal Chemistry Surface Markers of Cancer Stem Cells in Solid Tumors
Current Stem Cell Research & Therapy Editorial [Hot topic: Apoptosis in Carcinogenesis and Cancer Therapy (Guest Editors: George G. Chen and Paul B.S. Lai)]
Current Cancer Drug Targets