Abstract
Lanthanide complexes have been used for fluorescent labels to the wide variety of analytical methods including high performance liquid chromatography, immunoassay, and fluorescent imaging with time-resolved fluorometric measurements. Introduction of a time-resolved fluorometric measurement has been permitted the easy distinction of a specific long-lived fluorescence signal from short-lived natural background fluorescence present in most biological samples, reaction wells and optical components. Recently, new fluorescent labels and new instruments have been reported for timeresolved fluorescence including high performance liquid chromatography as in environmental analysis as river water, clinical chemistry as allergen assay, and a biochemical analysis as in staining method of proteins on polyacrylamide gel. The time-resolved fluorometric detection methods using new fluorescent labels improved the detection sensitivity compared to those of previously-described methods. This paper reviews those developments of applications of lanthanide fluorescent labels for various purposes: time-resolved fluorometric high performance liquid chromatography, timeresolved fluorescent immunoassay, and protein staining in recent years.
Keywords: Lanthanide complex, Fluorescent label, Time-resolved fluorescence, High-performance liquid hromatography, Immunoassay, Electrophoresis
Current Analytical Chemistry
Title: Design of Lanthanide Complex Probes for Highly Sensitive Time-Resolved Fluorometric Detection Methods and Its Application to Biochemical, Environmental and Clinical Analyses
Volume: 2 Issue: 4
Author(s): Takeshi Matsuya, Nobuhiro Hoshino and Tsuneo Okuyama
Affiliation:
Keywords: Lanthanide complex, Fluorescent label, Time-resolved fluorescence, High-performance liquid hromatography, Immunoassay, Electrophoresis
Abstract: Lanthanide complexes have been used for fluorescent labels to the wide variety of analytical methods including high performance liquid chromatography, immunoassay, and fluorescent imaging with time-resolved fluorometric measurements. Introduction of a time-resolved fluorometric measurement has been permitted the easy distinction of a specific long-lived fluorescence signal from short-lived natural background fluorescence present in most biological samples, reaction wells and optical components. Recently, new fluorescent labels and new instruments have been reported for timeresolved fluorescence including high performance liquid chromatography as in environmental analysis as river water, clinical chemistry as allergen assay, and a biochemical analysis as in staining method of proteins on polyacrylamide gel. The time-resolved fluorometric detection methods using new fluorescent labels improved the detection sensitivity compared to those of previously-described methods. This paper reviews those developments of applications of lanthanide fluorescent labels for various purposes: time-resolved fluorometric high performance liquid chromatography, timeresolved fluorescent immunoassay, and protein staining in recent years.
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Cite this article as:
Matsuya Takeshi, Hoshino Nobuhiro and Okuyama Tsuneo, Design of Lanthanide Complex Probes for Highly Sensitive Time-Resolved Fluorometric Detection Methods and Its Application to Biochemical, Environmental and Clinical Analyses, Current Analytical Chemistry 2006; 2 (4) . https://dx.doi.org/10.2174/157341106778520526
DOI https://dx.doi.org/10.2174/157341106778520526 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
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