Crystallization and Preliminary X-Ray Analysis of Fluorescent Protein mBanana

Title: Crystallization and Preliminary X-Ray Analysis of Fluorescent Protein mBanana

Volume: 15 Issue: 1

Author(s): Yu Ding, Yangbin Zhou, Yifeng Wu, Jiaping Song, Xiaojian Hu and Zhihong Zhang

Affiliation:

Keywords: mBanana, fluorescent protein, crystallization, preliminary X-ray analysis

Abstract: mBanana is a novel monomeric red fluorescent protein mutant. It was cloned and expressed in Escherichia coli with 10 histidine residues at its N-terminal. After cleavage of the His tag by TEV protease, the mBanana was further purified and crystallized by the hanging-drop vapor-diffusion technique. The crystals can diffract to 2.0Å resolution and one set of completed data was collected. It showed that the orthorhombic mBanana crystal was in space group P21 with unit cell parameters (48.629, 42.667, 61.714, 90, 111.676, 90) and contained one molecule in one asymmetric unit.

Cite this article as:

Ding Yu, Zhou Yangbin, Wu Yifeng, Song Jiaping, Hu Xiaojian and Zhang Zhihong, Crystallization and Preliminary X-Ray Analysis of Fluorescent Protein mBanana, Protein & Peptide Letters 2008; 15 (1) . https://dx.doi.org/10.2174/092986608783330341

DOI https://dx.doi.org/10.2174/092986608783330341	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305