Secondary Structure of the Homologous Proteins, A A-Fetoprotein and Serum Albumin, from their Circular Dichroism and Infrared Spectra

Title: Secondary Structure of the Homologous Proteins, A A-Fetoprotein and Serum Albumin, from their Circular Dichroism and Infrared Spectra

Volume: 8 Issue: 4

Author(s): Keith A. Oberg and Vladimir N. Uversky

Affiliation:

Keywords: Proteins Fetoprotein, Serum Albumin, Circular Dichroism, Human serum albumin (HSA)

Abstract: Secondary structure composition of two homologous proteins, alpha-fetoprotein and serum albumin, has been compared using circular dichroism and Fourier-transform infrared spectroscopy. CD-only analysis gives an accurate a-helical content for SA, but underestimates the predicted value for AFP. In contrast, FTIR-only analysis underestimates the helical content of SA. However, analysis of hybrid IR-CD spectra gives more accurate values for secondary structure composition for both AFP and SA and shows that the secondary structures of these proteins are the same.

Cite this article as:

Oberg A. Keith and Uversky N. Vladimir, Secondary Structure of the Homologous Proteins, A A-Fetoprotein and Serum Albumin, from their Circular Dichroism and Infrared Spectra, Protein & Peptide Letters 2001; 8 (4) . https://dx.doi.org/10.2174/0929866013409391