Using Siclopps for the Discovery of Novel Antimicrobial Peptides and Their Targets

Title: Using Siclopps for the Discovery of Novel Antimicrobial Peptides and Their Targets

Volume: 12 Issue: 8

Author(s): Lisa O. Nilsson, Mostafa Louassini and Ernesto Abel-Santos

Affiliation:

Keywords: cyclic peptides, antimicrobial, functional complementation

Abstract: High throughput screening of SICLOPPS libraries afforded six distinct cyclic peptides that inhibit Escherichia coli growth both in liquid and solid media. One of these peptides (LN05) reduced both bacterial growth rate and caused cell aggregation in liquid media. Mutant bacteria immune to LN05 action were obtained at a frequency of 10-7. Overexpression of an E. coli genomic library in the presence of LN05 production resulted in enrichment of a single genomic construct, a fragment of the NarZ gene.

Cite this article as:

Nilsson O. Lisa, Louassini Mostafa and Abel-Santos Ernesto, Using Siclopps for the Discovery of Novel Antimicrobial Peptides and Their Targets, Protein & Peptide Letters 2005; 12 (8) . https://dx.doi.org/10.2174/0929866054864247

DOI https://dx.doi.org/10.2174/0929866054864247	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305