Expressed Protein Ligation to Study Protein Interactions: Semi-Synthesis of the G-Protein Alpha Subunit

Title: Expressed Protein Ligation to Study Protein Interactions: Semi-Synthesis of the G-Protein Alpha Subunit

Volume: 12 Issue: 8

Author(s): Lori L. Anderson, Garland R. Marshall and Thomas J. Baranski

Affiliation:

Keywords: g protein, intein, expressed protein ligation, rhodopsin, transducin

Abstract: Interactions between G proteins and GPCRs are fundamental for transmitting signals for a multitude of physiologic responses. Little is known regarding the protein-protein interface between the G protein and the receptor, much less the mechanisms for receptor activation of G proteins. Here, we will describe how expressed protein ligation will aid in the study of protein-protein interactions between semi-synthetic G alpha subunits and GPCRs.

Cite this article as:

Anderson L. Lori, Marshall R. Garland and Baranski J. Thomas, Expressed Protein Ligation to Study Protein Interactions: Semi-Synthesis of the G-Protein Alpha Subunit, Protein & Peptide Letters 2005; 12 (8) . https://dx.doi.org/10.2174/0929866054864175

DOI https://dx.doi.org/10.2174/0929866054864175	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305