Expressed Protein Ligation to Obtain Selectively Modified Aldo/Keto Reductases

Title: Expressed Protein Ligation to Obtain Selectively Modified Aldo/Keto Reductases

Volume: 12 Issue: 8

Author(s): Michael P.O. Richter and Annette G. Beck-Sickinger

Affiliation:

Keywords: expressed protein ligation (epl), site specific covalent immobilization, akr a, artificial redoxproteins, aldo/keto reductase (akr)

Abstract: Specific enzyme immobilization has moved into the focus for many applications in biochemical research fields. Expressed Protein Ligation (EPL) has been proven to be ideal to selectively label proteins at single positions. Applying this technique to enzymes of the aldo/keto reductase superfamily provides a new approach to generate native or modified redox enzymes for direct and indirect immobilization.

Cite this article as:

Richter P.O. Michael and Beck-Sickinger G. Annette, Expressed Protein Ligation to Obtain Selectively Modified Aldo/Keto Reductases, Protein & Peptide Letters 2005; 12 (8) . https://dx.doi.org/10.2174/0929866054864210

DOI https://dx.doi.org/10.2174/0929866054864210	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305