Isolation and Partial Characterization of Ribonuclease Inhibitor from Goat Liver

Title: Isolation and Partial Characterization of Ribonuclease Inhibitor from Goat Liver

Volume: 13 Issue: 8

Author(s): Juin Chatterjee, Tushar Kanti Maiti and Swagata Dasgupta

Affiliation:

Keywords: Ribonuclease inhibitor, agarose gel based assay, angiogenesis, CAM assay

Abstract: Ribonuclease inhibitor (RI), a 50 kDa protein, has been found both in mammalian and nonmammalian tissues. We have isolated RI from goat liver and partial characterization has been accomplished. For the isolation of RI, DEAE cellulose column chromatography followed by affinity chromatography using CNBr activated Sepharose 4B was performed. The inhibition of ribonucleolytic activity of Ribonuclease A has been checked by an agarose gel based assay. The antiangiogenic property of the protein was tested by the chorioallantoic membrane (CAM) assay. Results indicate inhibition of angiogenesis.

Cite this article as:

Chatterjee Juin, Kanti Maiti Tushar and Dasgupta Swagata, Isolation and Partial Characterization of Ribonuclease Inhibitor from Goat Liver, Protein & Peptide Letters 2006; 13 (8) . https://dx.doi.org/10.2174/092986606777841307