Abstract
Ion exchange chromatography (IEC) poses stresses on proteins in both binding and elution steps. Proteins often bind to the column with high affinity, resulting in concentration of the protein upon binding. Elution often requires high salt concentration, leading to high protein concentration with high salt concentration. Although hydrophobic interaction chromatography (HIC) involves weak interaction, salting-out salts are used for binding. These conditions may cause protein aggregation. This short article describes an approach to reduce such aggregation in IEC and HIC. This was achieved by adding small amount of salt or arginine in the loading sample or elution solvent, resulting in elution of proteins with less aggregation or higher recovery.
Current Pharmaceutical Biotechnology
Title: Stress-Free Chromatography: IEC and HIC
Volume: 10 Issue: 4
Author(s): Tsutomu Arakawa, Yoshiko Kita and Daisuke Ejima
Affiliation:
Abstract: Ion exchange chromatography (IEC) poses stresses on proteins in both binding and elution steps. Proteins often bind to the column with high affinity, resulting in concentration of the protein upon binding. Elution often requires high salt concentration, leading to high protein concentration with high salt concentration. Although hydrophobic interaction chromatography (HIC) involves weak interaction, salting-out salts are used for binding. These conditions may cause protein aggregation. This short article describes an approach to reduce such aggregation in IEC and HIC. This was achieved by adding small amount of salt or arginine in the loading sample or elution solvent, resulting in elution of proteins with less aggregation or higher recovery.
Export Options
About this article
Cite this article as:
Arakawa Tsutomu, Kita Yoshiko and Ejima Daisuke, Stress-Free Chromatography: IEC and HIC, Current Pharmaceutical Biotechnology 2009; 10 (4) . https://dx.doi.org/10.2174/138920109788488879
DOI https://dx.doi.org/10.2174/138920109788488879 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Recent Advances in Neuroinflammation Therapeutics: PPARs/LXR as Neuroinflammatory Modulators
Current Pharmaceutical Design Editorial [Hot Topic: New Approaches to the Treatment of Viral Diseases (Guest Editor: Cristina Gardelli)]
Current Topics in Medicinal Chemistry Signal Transduction Pathways of Inflammatory Gene Expressions and Therapeutic Implications
Current Pharmaceutical Design Colorectal Cancer Epidemiology: Recent Trends and Impact on Outcomes
Current Drug Targets In Vitro and In Vivo Models of Multiple Sclerosis
CNS & Neurological Disorders - Drug Targets Immunomodulating and Anti-Allergic Effects of Negroamaro and Koshu Vitis vinifera Fermented Grape Marc (FGM)
Current Pharmaceutical Design Advances in Helper-Dependent Adenoviral Vector Research
Current Gene Therapy Testosterone as Potential Effective Therapy in Treatment of Obesity in Men with Testosterone Deficiency: A Review
Current Diabetes Reviews Lymphangiogenesis and Anti-Tumor Immune Responses
Current Molecular Medicine Therapeutic Potential of Nucleic Acid-Based Drugs in Coronary Hyper- Proliferative Vascular Diseases
Current Medicinal Chemistry Pharmacokinetics of Recombinant Human Endostatin in Rats
Current Drug Metabolism Weight Gain and Metabolic Changes During Treatment with Antipsychotics and Antidepressants
Endocrine, Metabolic & Immune Disorders - Drug Targets Glucagon Like Peptide-1 and Atherosclerosis
Cardiovascular & Hematological Agents in Medicinal Chemistry Adult Stem Cells and Skeletal Muscle Regeneration
Current Gene Therapy Synthesis and in vitro Evaluation of Polymeric Prodrug of Ibuprofen with Amino Acid Spacer
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Antileishmanial Patents Antileishmanial Current Drugs and Relevant Patents
Recent Patents on Anti-Infective Drug Discovery Hydrogen Sulfide in Inflammation: Friend or Foe?
Inflammation & Allergy - Drug Targets (Discontinued) Cancer-Associated Carbonic Anhydrases and Their Inhibition
Current Pharmaceutical Design Molecular Pathology of Pancreatic Cancer: From Bench-to-Bedside Translation
Current Drug Targets Patent Selections:
Recent Patents on Anti-Cancer Drug Discovery