A Differential Protein Study on Bronchoalveolar Lavage Fluid at Different Stages of Silicosis

Title:A Differential Protein Study on Bronchoalveolar Lavage Fluid at Different Stages of Silicosis

Volume: 27 Issue: 16

Author(s): Xiaoxuan Zhang, Ke Han, Linhui Kan, Zheng Zhang, Yihong Gong, Shuyu Xiao, Yuping Bai, Nan Liu, Chunyan Meng, Huisheng Qi*Fuhai Shen*

Affiliation:

• Tangshan City workers' Hospital, Tangshan, Hebei, 063000, P.R. China

• Hebei Province Key Laboratory of Occupational Health and Safety for Coal Industry, School of Public Health, North China University of Science and Technology, Tangshan, Hebei, 063210, P.R. China

Abstract:

Objectives: In this study, by comparing the difference in protein expression in bronchoalveolar lavage fluid between silicosis patients in different stages and healthy controls, the pathogenesis of pneumoconiosis was discussed, and a new idea for the prevention and treatment of pneumoconiosis was provided.

Methods: The lung lavage fluid was pretreated by 10 K ultrafiltration tube, Agilent 1100 conventional liquid phase separation, strong cation exchange column (SCX) HPLC pre-separation, and C18 reverse phase chromatography desalting purification, and protein was labeled with isotope. GO, KEGG pathway, and PPI analysis of differential proteins were conducted by bioinformatics, and protein types and corresponding signal pathways were obtained.

Results: Thermo Q-Exactive mass spectrometry identified 943 proteins. T-test analysis was used to evaluate the different significance of the results, and the different protein of each group was obtained by screening with the Ratio≥1.2 or Ratio≤0.83 and P<0.05. We found that there are 16 kinds of protein throughout the process of silicosis. There are different expressions of protein in stages III/control, stages II/control, stage I/control, stages III/ stages II, stages III/ stage I and stages II/ stage I groups. The results of ontology enrichment analysis of total differential protein genes show that KEGG pathway enrichment analysis of differential protein suggested that there were nine pathways related to silicosis.

Conclusion: The main biological changes in the early stage of silicosis are glycolysis or gluconeogenesis, autoimmunity, carbon metabolism, phagocytosis, etc., and microfibril-associated glycoprotein 4 may be involved in the early stage of silicosis. The main biological changes in the late stage of silicosis are autoimmunity, intercellular adhesion, etc. Calcium hippocampus binding protein may participate in the biological changes in the late stage of silicosis. It provides a new idea to understand the pathogenesis of silicosis and also raises new questions for follow-up research.

Cite this article as:

Zhang Xiaoxuan, Han Ke, Kan Linhui, Zhang Zheng, Gong Yihong, Xiao Shuyu, Bai Yuping, Liu Nan, Meng Chunyan, Qi Huisheng*, Shen Fuhai*, A Differential Protein Study on Bronchoalveolar Lavage Fluid at Different Stages of Silicosis, Combinatorial Chemistry & High Throughput Screening 2024; 27 (16) . https://dx.doi.org/10.2174/0113862073260760231023055036

DOI https://dx.doi.org/10.2174/0113862073260760231023055036	Print ISSN 1386-2073
Publisher Name Bentham Science Publisher	Online ISSN 1875-5402