Login Register Cart 0
Generic placeholder image

Current Chemical Biology

Editor-in-Chief

ISSN (Print): 2212-7968
ISSN (Online): 1872-3136

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Exploring the Therapeutic Potential: Antiplatelet and Antioxidant Activities of Some Medicinal Plants in Morocco

Author(s): Fatima Zahra Lafdil, Asmae Amirou, Mohamed Bnouham, Abdelkhaleq Legssyer, Abderrahim Ziyyat and Hassane Mekhfi*

Volume 17, Issue 4, 2023

Published on: 19 December, 2023

Page: [237 - 248] Pages: 12

DOI: 10.2174/0122127968271295231212043119

Price: $65

Abstract

Background: Thrombotic events and oxidative stress are major complications of certain ischemic disorders. The fight against these complications requires very intense research to develop new therapeutic agents of natural origin.

Objective: The general objective of this work is the scientific valorization of five medicinal plants: Rhus pentaphylla, Zizyphus lotus, Ammodaucus leucotrichus, Inula viscosa, and Cinnamomum zeylanicum by exploring their effects on rat platelet aggregation, antioxidant potential and determining their phytochemical composition.

Methodology: The aggregation test was monitored by stimulating isolated washed platelets suspension in the absence and presence of extracts. The antioxidant activity was conducted in vitro according to three methods: DPPH free radical scavenging activity, β-carotene bleaching test, and ferric reducing antioxidant power test. The quantitative determination of total polyphenols and flavonoids are determined respectively according to the Folin-Ciocalteu method and the colorimetric method with aluminum chloride.

Results: The results obtained show that the aqueous extract of the fruits of Rhus pentaphylla and the aerial part of Inula viscosa, as well as the stalk peel of Cinnamomum zeylanicum, significantly (p<0.001) inhibit thrombin-platelet aggregation, while the other plant extracts have a slightly, but significant effect. These extracts exert a remarkable antioxidant activity with the three methods used. But, their IC50 values are still higher than those of the antioxidant references (ascorbic acid and butyl hydroxyanisole). Qualitative phytochemical analysis revealed the presence of secondary metabolites with varying contents. Additionally, the results of quantitative phytochemical analysis showed that the aqueous extracts of the leaves of Rhus pentaphylla and the aerial part of Inula viscosa contain the highest amount of polyphenols and flavonoids. These secondary metabolites are also present in the other extracts but in smaller quantities.

Conclusion: These results could contribute to the validation of the medical use of these extracts that exert an antiplatelet effect to treat hemostatic and thrombotic disorders.

« Previous Next »
Graphical Abstract

[1]
Lasne, D.; Jude, B.; Susen, S. From normal to pathological hemostasis. Can. J. Anaesth., 2006, 53(S2), S2-S11.
[http://dx.doi.org/10.1007/BF03022247] [PMID: 16766787]
[2]
Spasov, A.A.; Kucheryavenko, A.F.; Kosolapov, V.A.; Anisimova, V.A. Antithrombogenic activity of antioxidant compounds. Bull. Exp. Biol. Med., 2013, 155(6), 775-777.
[http://dx.doi.org/10.1007/s10517-013-2250-x] [PMID: 24288764]
[3]
Kubatka, P.; Mazurakova, A.; Koklesova, L.; Samec, M.; Sokol, J.; Samuel, S.M.; Kudela, E.; Biringer, K.; Bugos, O.; Pec, M.; Link, B.; Adamkov, M.; Smejkal, K.; Büsselberg, D.; Golubnitschaja, O. Antithrombotic and antiplatelet effects of plant-derived compounds: A great utility potential for primary, secondary, and tertiary care in the framework of 3P medicine. EPMA J., 2022, 13(3), 407-431.
[http://dx.doi.org/10.1007/s13167-022-00293-2] [PMID: 35990779]
[4]
Nguyen, T.M.H.; Le, H.L.; Ha, T.T.; Bui, B.H.; Le, N.T.; Nguyen, V.H.; Nguyen, T.V.A. Inhibitory effect on human platelet aggregation and coagulation and antioxidant activity of C. edulis Ker Gawl rhizome and its secondary metabolites. J. Ethnopharmacol., 2020, 263(August), 113136.
[http://dx.doi.org/10.1016/j.jep.2020.113136] [PMID: 32758576]
[5]
Campbell, C.L.; Smyth, S.; Montalescot, G.; Steinhubl, S.R. Aspirin dose for the prevention a systematic review. JAMA, 2007, 297(18), 2018-2024.
[http://dx.doi.org/10.1001/jama.297.18.2018] [PMID: 17488967]
[6]
Ziyyat, A.; Legssyer, A.; Mekhfi, H.; Dassouli, A.; Serhrouchni, M.; Benjelloun, W. Phytotherapy of hypertension and diabetes in oriental Morocco. J. Ethnopharmacol., 1997, 58(1), 45-54.
[http://dx.doi.org/10.1016/S0378-8741(97)00077-9] [PMID: 9324004]
[7]
El Hafian, M.; Benlandini, N.; Elyacoubi, H.; Zidane, L.; Rochdi, A. Floristic and ethnobotanical study of medicinal plants used in the prefecture of Agadir-Ida-Outanane (Morocco). J. Appl. Biosci., 2014, 81(1), 7198.
[http://dx.doi.org/10.4314/jab.v81i1.8]
[8]
Mary, N.K.; Achuthan, C.R.; Babu, B.H.; Padikkala, J. In vitro antioxidant and antithrombotic activity of Hemidesmus indicus (L) R. Br. J. Ethnopharmacol., 2003, 87(2-3), 187-191.
[http://dx.doi.org/10.1016/S0378-8741(03)00119-3] [PMID: 12860306]
[9]
Bamm, J. Medicinal plants in the Achach forest (central plateau, Morocco). Acta Bot. Malacit., 2002, 27, 131-145.
[10]
Ouhaddou, H.; Boubaker, H.; Msanda, F.; El Mousadik, A. An ethnobotanical study of medicinal plants of the Agadir Ida Ou Tanane province (southwest Morocco). J. Appl. Biosci., 2015, 84(1), 7707.
[http://dx.doi.org/10.4314/jab.v84i1.5]
[11]
ElHaouari, M.; ElMakaoui, S.; Jnah, M.; Haddaouy, A. A survey of medicinal plants used by herbalists in Taza (Northern Morocco) to manage various ailments. J. Mater. Environ. Sci., 2018, 9(6), 1875-1888.
[http://dx.doi.org/10.26872/jmes.2018.9.6.207]
[12]
Raal, A.; Meos, A.; Hinrikus, T.; Heinämäki, J. Romāne, E.; Gudienė V.; Jak Tas, V.; Koshovyi, O.; Kovaleva, A.; Fursenco, C.; Chiru, T.; Nguyen, H.T. Dragendorff’s reagent: Historical perspectives and current status of a versatile reagent introduced over 150 years ago at the University of Dorpat, Tartu, Estonia. Pharmazie, 2020, 75(7), 299-306.
[http://dx.doi.org/10.1691/ph.2020.0438] [PMID: 32635970]
[13]
Buvaneswari, K.; Ramamoorthy, D.; Velanganni, J. Preliminary phytochemical and antimicrobial activity studies on the leaves of the indian plant thevetia neriifolia juss. kanchimamunivar centre for post graduate studies. Puducherry, India, 2011, 7(6), 659-666.
[14]
Jesus, R.S.; Piana, M.; Freitas, R.B.; Brum, T.F.; Alves, C.F.S.; Belke, B.V.; Mossmann, N.J.; Cruz, R.C.; Santos, R.C.V.; Dalmolin, T.V.; Bianchini, B.V.; Campos, M.M.A.; Bauermann, L.F. In vitro antimicrobial and antimycobacterial activity and HPLC–DAD screening of phenolics from Chenopodium ambrosioides L. Braz. J. Microbiol., 2018, 49(2), 296-302.
[http://dx.doi.org/10.1016/j.bjm.2017.02.012] [PMID: 29037505]
[15]
Hagerman, A.E. Extraction of tannin from fresh and preserved leaves. J. Chem. Ecol., 1988, 14(2), 453-461.
[http://dx.doi.org/10.1007/BF01013897] [PMID: 24275992]
[16]
Mu, H.; Battsetseg, B.; Ito, T.Y.; Otani, S.; Onishi, K.; Kurozawa, Y. Drug polymorphism: A review. Int. J. Health Sci., 2008.
[17]
de la Rosa, L.A.; Alvarez-Parrilla, E.; Shahidi, F. Phenolic compounds and antioxidant activity of kernels and shells of Mexican pecan (Carya illinoinensis). J. Agric. Food Chem., 2011, 59(1), 152-162.
[http://dx.doi.org/10.1021/jf1034306] [PMID: 21138247]
[18]
Lafdil, F.Z. Argania spinosa leaves and branches: Antiaggregant, anticoagulant, antioxidant activities and bioactive compounds quantification. J. Exp. Biol. Agric. Sci., 2023, 11(2320), 650-662.
[19]
Zhang, Q.; Ames, J.M.; Smith, R.D.; Baynes, J.W.; Metz, T.O. A perspective on the Maillard reaction and the analysis of protein glycation by mass spectrometry: probing the pathogenesis of chronic disease. J. Proteome Res., 2009, 8(2), 754-769.
[http://dx.doi.org/10.1021/pr800858h] [PMID: 19093874]
[20]
Kandsi, F.; Elbouzidi, A.; Lafdil, F.Z.; Meskali, N.; Azghar, A.; Addi, M.; Hano, C.; Maleb, A.; Gseyra, N. Antibacterial and antioxidant activity of Dysphania ambrosioides (L.) mosyakin and clemants essential oils: Experimental and computational approaches. Antibiotics, 2022, 11(4), 482.
[http://dx.doi.org/10.3390/antibiotics11040482] [PMID: 35453233]
[21]
Seyed Mohammad, N.; Dehpour, A.A.; Ebrahimzadeh, M.A.; Seyed Fazel, N. Antioxidant activity of the methanol extract of Ferula assafoetida and its essential oil composition. Grasas Aceites, 2009, 60(4), 405-412.
[http://dx.doi.org/10.3989/gya.010109]
[22]
Mekhfi, H.; Gadi, D.; Bnouham, M.; Ziyyat, A.; Legssyer, A.; Aziz, M. Effect of argan oil on platelet aggregation and bleeding time: A beneficial nutritional property. J. Complement. Integr. Med., 2008, 5(1)
[http://dx.doi.org/10.2202/1553-3840.1164]
[23]
Amirou, A. Effects of juglans regia root bark extract on platelet aggregation, bleeding time, and plasmatic coagulation: In vitro and ex vivo experiments. Evidence-based Complement. Altern. Med., 2018, 2018, 7313517.
[http://dx.doi.org/10.1155/2018/7313517]
[24]
Cen, C.; Fengqin, W.; Wen, X.; Zhining, X.; Guang, H.; Jianbo, W.; Fengqing, Y. Effect on platelet aggregation activity: extracts from 31 Traditional Chinese Medicines with the property of activating blood and resolving stasis. J. Tradit. Chin. Med., 2017, 37(1), 64-75.
[http://dx.doi.org/10.1016/S0254-6272(17)30028-6] [PMID: 29957905]
[25]
Mekhfi, H.; Haouari, M.E.; Legssyer, A.; Bnouham, M.; Aziz, M.; Atmani, F.; Remmal, A.; Ziyyat, A. Platelet anti-aggregant property of some Moroccan medicinal plants. J. Ethnopharmacol., 2004, 94(2-3), 317-322.
[http://dx.doi.org/10.1016/j.jep.2004.06.005] [PMID: 15325737]
[26]
Amirou, A. Effects of walnut bark extract on the human platelet aggregation, adhesion, and plasmatic coagulation in vitro. Adv. Pharmacol. Pharm. Sci., 2023, 2023, 5644803.
[27]
Esmon, C.T. The interactions between inflammation and coagulation. Br. J. Haematol., 2005, 131(4), 417-430.
[http://dx.doi.org/10.1111/j.1365-2141.2005.05753.x] [PMID: 16281932]
[28]
Khorana, A.A.; Francis, C.W.; Culakova, E.; Kuderer, N.M.; Lyman, G.H. Thromboembolism is a leading cause of death in cancer patients receiving outpatient chemotherapy. J. Thromb. Haemost., 2007, 5(3), 632-634.
[http://dx.doi.org/10.1111/j.1538-7836.2007.02374.x] [PMID: 17319909]
[29]
Paydas, S. Management of adverse effects/toxicity of ibrutinib. Crit. Rev. Oncol. Hematol., 2019, 136, 56-63.
[http://dx.doi.org/10.1016/j.critrevonc.2019.02.001] [PMID: 30878129]
[30]
Karcioglu, O.; Sehmus, Z.; Bilgen, O. Direct (new) oral anticoagulants (DOACs): Drawbacks, bleeding and reversal. Cardiovasc. Hematol. Agents Med. Chem., 2022, 20(2), 103-113.
[31]
Karunamoorthi, K.; Jegajeevanram, K.; Vijayalakshmi, J.; Mengistie, E. Traditional medicinal plants: A source of phytotherapeutic modality in resource-constrained health care settings. J. Evid. Based Complementary Altern. Med., 2013, 18(1), 67-74.
[http://dx.doi.org/10.1177/2156587212460241]
[32]
Singh, I.; Mok, M.; Christensen, A.M.; Turner, A.H.; Hawley, J.A. The effects of polyphenols in olive leaves on platelet function. Nutr. Metab. Cardiovasc. Dis., 2008, 18(2), 127-132.
[http://dx.doi.org/10.1016/j.numecd.2006.09.001] [PMID: 17346951]
[33]
Salonen, J.T. Antioxidants and platelets. Ann. Med., 1989, 21(1), 59-62.
[http://dx.doi.org/10.3109/07853898909149183] [PMID: 2923705]
[34]
Barros, L.; Pereira, E.; Calhelha, R.C.; Dueñas, M.; Carvalho, A.M.; Santos-Buelga, C.; Ferreira, I.C.F.R. Bioactivity and chemical characterization in hydrophilic and lipophilic compounds of Chenopodium ambrosioides L. J. Funct. Foods, 2013, 5(4), 1732-1740.
[http://dx.doi.org/10.1016/j.jff.2013.07.019]
[35]
Tchani, G.W.; Agbeme, K.S.; Agbodan, K.A.; Baba, G.; Kpegba, K. Phytochemical study and comparative antioxidant activity of extracts from aerial parts of chenopodium ambrosioides linn. (Chenopodiaceae). Adv. Biol. Chem., 2021, 11(5), 220-233.
[http://dx.doi.org/10.4236/abc.2021.115015]
[36]
Shah, H.; Khan, A.A. Phytochemical characterisation of an important medicinal plant, Chenopodium ambrosioides Linn. Nat. Prod. Res., 2017, 31(19), 2321-2324.
[http://dx.doi.org/10.1080/14786419.2017.1299722] [PMID: 28288517]
[37]
Subedi, L.; Timalsena, S.; Duwadi, P.; Thapa, R.; Paudel, A.; Parajuli, K. Antioxidant activity and phenol and flavonoid contents of eight medicinal plants from Western Nepal. J. Tradit. Chin. Med., 2014, 34(5), 584-590.
[http://dx.doi.org/10.1016/S0254-6272(15)30067-4] [PMID: 25417410]
[38]
Martin, S.; Andriantsitohaina, R. Cellular mechanism of vasculo-protection induced by polyphenols on the endothelium. Ann. Cardiol. d’angéiologie, 2002, 51(6), 304-315.
[http://dx.doi.org/10.1016/S0003-3928(02)00138-5]
[39]
Devaraj, V.C.; Krishna, B.G. Antiulcer activity of a polyherbal formulation (PHF) from Indian medicinal plants. Chin. J. Nat. Med., 2013, 11(2), 145-148.
[http://dx.doi.org/10.1016/S1875-5364(13)60041-2] [PMID: 23787181]
[40]
Pandey, A.K.; Tyagi, C.K.; Shah, S.K.; Tiwari, S.M.; Rawat, P.K.; Sahu, G.D. Formulation development and evaluation of directly compressed polyherbal tablets for the management of infections caused by helminthes. Mater. Today Proc., 2023, 80, 3532-3539.
[http://dx.doi.org/10.1016/j.matpr.2021.07.291]
[41]
Kurhajec, S.; Kostelanská, K.; Pavloková, S.; Vetchý, D.; Wolaschka, T.; Gajdziok, J.; Franc, A. Stabilized antioxidative plant extracts formulated by liquisolid technique. J. Drug Deliv. Sci. Technol., 2020, 60, 102022.
[http://dx.doi.org/10.1016/j.jddst.2020.102022]
[42]
Abullais Saquib, S.; Abdullah AlQahtani, N.; Ahmad, I.; Arora, S.; Mohammed Asif, S.; Ahmed Javali, M.; Nisar, N. Synergistic antibacterial activity of herbal extracts with antibiotics on bacteria responsible for periodontitis. J. Infect. Dev. Ctries., 2021, 15(11), 1685-1693.
[http://dx.doi.org/10.3855/jidc.14904] [PMID: 34898497]
[43]
Sierpina, V.S.; Wollschlaeger, B.; Blumenthal, M. Ginkgo biloba. Am. Fam. Physician, 2003, 68(5), 923-926.
[PMID: 13678141]
[44]
Middleton, E., Jr; Kandaswami, C.; Theoharides, T.C. The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacol. Rev., 2000, 52(4), 673-751.
[PMID: 11121513]
[45]
Chahmi, N.; Anissi, J.; Jennan, S.; Farah, A.; Sendide, K.; Hassouni, M.E. Antioxidant activities and total phenol content of Inula viscosa extracts selected from three regions of Morocco. Asian Pac. J. Trop. Biomed., 2015, 5(3), 228-233.
[http://dx.doi.org/10.1016/S2221-1691(15)30010-1]
[46]
Sharifi-Rad, J.; Quispe, C.; Zam, W.; Kumar, M.; Cardoso, S.M.; Pereira, O.R.; Ademiluyi, A.O.; Adeleke, O.; Moreira, A.C. Živković J.; Noriega, F.; Ayatollahi, S.A.; Kobarfard, F.; Faizi, M.; Martorell, M.; Cruz-Martins, N.; Butnariu, M.; Bagiu, I.C.; Bagiu, R.V.; Alshehri, M.M.; Cho, W.C. Phenolic bioactives as antiplatelet aggregation factors: The pivotal ingredients in maintaining cardiovascular health. Oxid. Med. Cell. Longev., 2021, 2021, 1-19.
[http://dx.doi.org/10.1155/2021/2195902] [PMID: 34447485]
[47]
Manna, S.K.; Mukhopadhyay, A.; Aggarwal, B.B. Resveratrol suppresses TNF-induced activation of nuclear transcription factors NF-κ B, activator protein-1, and apoptosis: Potential role of reactive oxygen intermediates and lipid peroxidation. J. Immunol., 2000, 164(12), 6509-6519.
[http://dx.doi.org/10.4049/jimmunol.164.12.6509] [PMID: 10843709]
[48]
Nardini, M.; Natella, F.; Scaccini, C. Role of dietary polyphenols in platelet aggregation. A review of the supplementation studies. Platelets, 2007, 18(3), 224-243.
[http://dx.doi.org/10.1080/09537100601078083] [PMID: 17497435]
[49]
Fernandez-Panchon, M.S.; Villano, D.; Troncoso, A.M.; Garcia-Parrilla, M.C. Antioxidant activity of phenolic compounds: From in vitro results to in vivo evidence. Crit. Rev. Food Sci. Nutr., 2008, 48(7), 649-671.
[http://dx.doi.org/10.1080/10408390701761845] [PMID: 18663616]
[50]
Pandey, A.K.; Mishra, A.K.; Mishra, A. Antifungal and antioxidative potential of oil and extracts derived from leaves of Indian spice plant Cinnamomum tamala. Cell. Mol. Biol., 2012, 58(1), 142-147.
[PMID: 23273204]
[51]
Mishra, A.; Sharma, A.K.; Kumar, S.; Saxena, A.K.; Pandey, A.K. Bauhinia variegata leaf extracts exhibit considerable antibacterial, antioxidant, and anticancer activities. BioMed Res. Int., 2013, 2013, 915436.
[52]
Tosetti, F.; Noonan, D.M.; Albini, A. Metabolic regulation and redox activity as mechanisms for angioprevention by dietary phytochemicals. Int. J. Cancer, 2009, 125(9), 1997-2003.
[http://dx.doi.org/10.1002/ijc.24677] [PMID: 19551861]
[53]
Upadhyay, S.; Dixit, M. Role of polyphenols and other phytochemicals on molecular signaling. Oxid. Med. Cell. Longev., 2015, 2015, 504253.

Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy