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Protein & Peptide Letters

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ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

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Research Article

Water-soluble Moringa oleifera Seed Lectin Exhibits Monoaminergic Pathway-linked Anti-depressive-like Effects in Mice

Author(s): Leydianne Leite de Siqueira Patriota*, Barbara Raíssa Ferreira de Lima, Amanda de Oliveira Marinho, Jainaldo Alves da Costa, Luana Cassandra Breitenbach Barroso Coelho, Moacyr Jesus Barreto de Melo Rêgo, Maira Galdino da Rocha Pitta, Patrícia Maria Guedes Paiva, Michelly Cristiny Pereira, Thiago Henrique Napoleão and Michelle Melgarejo da Rosa

Volume 30, Issue 12, 2023

Published on: 21 November, 2023

Page: [1048 - 1057] Pages: 10

DOI: 10.2174/0109298665270366231031052629

Price: $65

Abstract

Objectives: The present study investigated the anti-depressive-like (anti-immobility) effect of a lectin from Moringa oleifera seeds (WSMoL) in mice.

Methods: To evaluate an acute effect, the animals were treated with WSMoL (1, 2, and 4 mg/kg, i.p.) 30 min before the tail suspension test (TST). To investigate the involvement of monoaminergic and nitrergic signaling, the mice were pre-treated with selective antagonists. The role of the WSMoL carbohydrate-recognizing domain (CRD) was verified using previous blockage with casein (0.5 mg/mL). The subacute anti-immobility effect was also evaluated by administering WSMoL (1, 2, and 4 mg/kg, i.p.) once a day for 7 d. Finally, an open field test (OFT) was performed to identify possible interferences of WSMoL on animal locomotory behavior.

Results: WSMoL reduced the immobility time of mice in the TST at all doses, and combined treatment with fluoxetine (5 mg/kg, i.p.) and WSMoL (1 mg/kg) was also effective. The CRD appeared to be involved in the anti-immobility effect since the solution of WSMoL (4 mg/kg) pre-incubated with casein showed no activity. The lectin effect was prevented by the pre-treatment of mice with ketanserin, yohimbine, and SCH 23390, thereby demonstrating the involvement of monoaminergic pathways. In contrast, pre-treatment with L-NAME, aminoguanidine, and L-arginine did not interfere with lectin action. WSMoL exhibited a subacute effect in the TST, thereby reducing immobility time and increasing agitation time even on the seventh day. OFT data revealed that the anti-immobility effect was not caused by interference with locomotor behavior.

Conclusion: WSMoL elicits an anti-depressant-like effect that is dependent on monoaminergic signaling.

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Graphical Abstract

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