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Current Bioactive Compounds

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ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

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Research Article

Evaluation of Antioxidant and Anticancer Activities of Memecylon umbellatum in Breast Carcinoma and Lupeol and Beta-sitosterol Quantified by HPTLC

Author(s): Shilpee Chaudhary, Chandrashekar Kodangala Subraya*, Sreedhara Ranganath Karkala, Vasudev Pai, Aswatha Ram Holavanna Halli Nanjundaiah and Aravinda Pai

Volume 20, Issue 5, 2024

Published on: 10 October, 2023

Article ID: e101023222021 Pages: 8

DOI: 10.2174/0115734072268707230921102959

Price: $65

Abstract

Introduction: Memecylon umbellatum is a perennial plant of the Melastomataceae family that grows in abundance in the Western Ghats and has been used traditionally to treat cancer and inflammatory conditions.

Methods: From the literature survey, it was found that no substantial work has been carried out to identify the bioactive compounds by HPTLC method and to screen the antioxidant and anticancer activity of M. umbellatum. Hence, an effort has been made to carry out the phytochemical investigation by HPTLC method and to screen the antioxidant activity by ABTS assay, DPPH assay, Nitric oxide antioxidant assay, Iron chelating activity and Total antioxidant activity.

Results: Total phenolics and flavonoids were carried out by using suitable methods. MCF-7 as well as MDA-MB-231 cells were used to test anticancer activities in vitro. Cell cycle analysis demonstrated that ethyl acetate extract caused G0/G1 phase arrest in MCF-7 cells. HPTLC analysis led to the identification of Lupeol from ethyl acetate extract.

Conclusion: The anticancer activity of ethyl acetate extract of M. umbellatum was significant. The antioxidant and anticancer activity may attributed to Lupeol, Phenolic compounds and Flavonoids.

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