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Pharmaceutical Nanotechnology

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ISSN (Print): 2211-7385
ISSN (Online): 2211-7393

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Research Article

Green Synthesis of Silver Nanoparticles using Morinda citrifolia Linn Leaf Extract and its Antioxidant, Antibacterial and Anticancer Potential

Author(s): Kailas D. Datkhile*, Shuvronil Chakraborty, Pratik P. Durgawale and Satish R. Patil

Volume 12, Issue 4, 2024

Published on: 10 October, 2023

Page: [340 - 350] Pages: 11

DOI: 10.2174/2211738511666230913095001

Price: $65

Abstract

Introduction: Nanomedicine has emerged as a revolutionary regimen for moderating communicable as well as non-communicable diseases.

Purpose: This study demonstrated the phytosynthesis of silver nanoparticles using M. citrifolia leaf extract (MC-AgNPs) and their in vitro antioxidant, antibacterial and anticancer potential.

Materials and Methods: The Biosynthesis of MC-AgNPs was studied by spectroscopy and characterized by SEM, TEM, XRD and FTIR analysis. The antibacterial activity was checked by minimum inhibition concentration assay. The HeLa and MCF-7 cancer cell lines were used to explore the cytotoxicity and genotoxicity activity of biogenic MC-AgNPs.

Results: The free radical scavenging potential of MC-AgNPs was studied by in vitro DPPH and ABTS assays, which confirmed significant radical scavenging activity in a dose-dependent manner with IC50 of 17.70 ± 0.36 μg/mL for DPPH and 13.37 ± 3.15 μg/mL for ABTS radicals. The bactericidal effects of MC-AgNPs confirmed by MIC showed 0.1 mg/mL concentration of MC-AgNPs with greater sensitivity for E.coli (93.33 ± 0.89), followed by K. pneumoniae (90.99 ± 0.57), S. aureus (87.26 ± 2.80) and P. aeruginosa strains (44.68 ± 0.73). The cytotoxicity results depicted strong dose and timedependent toxicity of biogenic MC-AgNPs against cancer cell lines fifty percent inhibitory concentration MC-AgNPs against HeLa cells were 13.56 ± 1.22 μg/mL after 24h and 5.57 ± 0.12 μg/mL after 48 h exposure, likewise 16.86 ± 0.88 μg/mL and 11.60 ± 0.97 μg/mL respectively for MCF-7 cells.

Conclusions: The present study revealed the green synthesis of silver nanoparticles using M. citrifolia and their significant antioxidant, antibacterial and anticancer activities.

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