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Pharmaceutical Nanotechnology

Editor-in-Chief

ISSN (Print): 2211-7385
ISSN (Online): 2211-7393

Research Article

Nanoemulsion of Ethanolic Extract of Centella asiatica (NanoSECA) Ameliorates Learning and Memory Performance by Enhancing Cholinergic Activities, Increasing Antioxidative Levels, and Attenuating Oxidative Stress Markers in Adult Rats

Author(s): Nor Atiqah Jusril, Shahida Muhammad Mukhtar, Syahrul Imran Abu Bakar, Wan Mazlina Md Saad, Ng Kwok Wen and Mohd Ilham Adenan*

Volume 12, Issue 4, 2024

Published on: 21 September, 2023

Page: [329 - 339] Pages: 11

DOI: 10.2174/2211738511666230831122844

Price: $65

Abstract

Background: Centella asiatica (C. asiatica) has long been traditionally used as a memory enhancer. Nanoemulsion of ethanolic extract C. asiatica (NanoSECA) has been developed to improve brain functions. However, the effect of NanoSECA on enhancing memory and cognitive functions remains unexplored.

Objectives: This research aimed to investigate the potential of NanoSECA on cognitive tasks and memory enhancement pathways in a normal adult rat model.

Methods: Thirty male Sprague Dawley rats (7-8 weeks old) were randomly subjected to five groups (n=six per group). Treatment groups were supplemented with NanoSECA and ethanolic extract of C. asiatica (SECA) for 28 days by oral gavages. Different brain sections were isolated, homogenized, and tested for acetylcholinesterase, antioxidants (glutathione and malondialdehyde), and anti-inflammatory agents (nitric oxide, tumour necrosis factor-α, and prostaglandin E2).

Results: NanoSECA supplementation markedly enhanced the acetylcholine, glutathione levels and reduced a distinct diminution in plasma activities of acetylcholinesterase, malondialdehyde, nitric oxide, prostaglandin E, and tumor necrosis factor-α levels.

Conclusion: NanoSECA can be used as a memory enhancer through enhanced cholinergic activity, increased antioxidant level, and reduced oxidative stress.

Graphical Abstract

[1]
Bensalem J, Dudonné S, Gaudout D, et al. Polyphenol-rich extract from grape and blueberry attenuates cognitive decline and improves neuronal function in aged mice. J Nutr Sci 2018; 7: e19.
[http://dx.doi.org/10.1017/jns.2018.10] [PMID: 29854398]
[2]
Sirichoat A, Chaijaroonkhanarak W, Prachaney P, et al. Effects of asiatic acid on spatial working memory and cell proliferation in the adult rat hippocampus. Nutrients 2015; 7(10): 8413-23.
[http://dx.doi.org/10.3390/nu7105401] [PMID: 26445061]
[3]
Kim HJ, Nel AE. The role of phase II antioxidant enzymes in protecting memory T cells from spontaneous apoptosis in young and old mice. J Immunol 2005; 175(5): 2948-59.
[http://dx.doi.org/10.4049/jimmunol.175.5.2948] [PMID: 16116181]
[4]
Wong JH, Muthuraju S, Reza F, et al. Differential expression of entorhinal cortex and hippocampal subfields α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptors enhanced learning and memory of rats following administration of Centella asiatica. Biomed Pharmacother 2019; 110: 168-80.
[http://dx.doi.org/10.1016/j.biopha.2018.11.044] [PMID: 30469081]
[5]
Sari DCR, Aswin S, Susilowati R, et al. Ethanol extracts of Centella asiatica leaf improves memory performance in rats after chronic stress via reducing nitric oxide and increasing Brain-Derived Neurotrophic Factor (BDNF) Concentration. GSTF Journal of Psychology (JPsych) 2014; 1(1): 9. [JPsych].
[http://dx.doi.org/10.7603/s40790-014-0009-0]
[6]
Gray NE, Zweig JA, Murchison C, et al. Centella asiatica attenuates Aβ-induced neurodegenerative spine loss and dendritic simplification. Neurosci Lett 2017; 646: 24-9.
[http://dx.doi.org/10.1016/j.neulet.2017.02.072] [PMID: 28279707]
[7]
Rahim NS, Lim SM, Mani V, Abdul Majeed AB, Ramasamy K. Enhanced memory in Wistar rats by virgin coconut oil is associated with increased antioxidative, cholinergic activities and reduced oxidative stress. Pharm Biol 2017; 55(1): 825-32.
[http://dx.doi.org/10.1080/13880209.2017.1280688] [PMID: 28118770]
[8]
Frater J, Lie D, Bartlett P, McGrath JJ. Insulin-like growth factor 1 (IGF-1) as a marker of cognitive decline in normal ageing: A review. Ageing Res Rev 2018; 42: 14-27.
[http://dx.doi.org/10.1016/j.arr.2017.12.002] [PMID: 29233786]
[9]
Chakravarthi K, Avadhani R. Beneficial effect of aqueous root extract of Glycyrrhiza glabra on learning and memory using different behavioral models: An experimental study. J Nat Sci Biol Med 2013; 4(2): 420-5.
[http://dx.doi.org/10.4103/0976-9668.117025] [PMID: 24082744]
[10]
Yolanda DA, Sari DCR, Rochmah MA, Suharmi S. The dose variations effect of Centella asiatica ethanol extract on escape latency’s distance morris water maze after chronic electrical stress. KnE Life Sciences 2015; 146-53.
[http://dx.doi.org/10.18502/kls.v2i1.134]
[11]
Moon M, Jeong HU, Choi JG, et al. Memory-enhancing effects of cuscuta japonica choisy via enhancement of adult hippocampal neurogenesis in mice. Behav Brain Res 2016; 311: 173-82.
[http://dx.doi.org/10.1016/j.bbr.2016.05.031] [PMID: 27185736]
[12]
Hafiz Z, Shamsuddin N, M, Mukhtar S, J, James R, I, Adenan M. Anti-acetylcholinesterase, anti-inflammatory and anti-oxidant activities of raw-extract Centella asiatica (RECA) on lipopolysaccharide (LPS)-induced neuroinflammation Sprague dawley rats. IACSIT Int J Eng Technol 2019; 7(4.14): 96-101.
[http://dx.doi.org/10.14419/ijet.v7i4.14.27479]
[13]
Marucci G, Buccioni M, Ben DD, Lambertucci C, Volpini R, Amenta F. Efficacy of acetylcholinesterase inhibitors in Alzheimer’s disease. Neuropharmacology 2021; 190: 108352.
[http://dx.doi.org/10.1016/j.neuropharm.2020.108352] [PMID: 33035532]
[14]
Headland SE, Norling LV. The resolution of inflammation: Principles and challenges. Semin Immunol 2015; 27(3): 149-60.
[http://dx.doi.org/10.1016/j.smim.2015.03.014] [PMID: 25911383]
[15]
Lala RR, Patel HP. Nanoemulsion for improved permeability of Centella asiatica extract: formulation, ex-vivo and in-vivo evaluation. Int J Pharm Sci Res 2019; 10(4): 1711-8.
[16]
Barai P, Raval N, Acharya S, Acharya N. Bergenia ciliata ameliorates streptozotocin-induced spatial memory deficits through dual cholinesterase inhibition and attenuation of oxidative stress in rats. Biomed Pharmacother 2018; 102: 966-80.
[http://dx.doi.org/10.1016/j.biopha.2018.03.115] [PMID: 29710552]
[17]
Howard B. National research council. In: District of Columbia. United States: Washington 1996; 26.(3)
[18]
Binti Mohd Yusuf Yeo NA, Muthuraju S, Wong JH, et al. Hippocampal amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid GluA1 (AMPA GluA1) receptor subunit involves in learning and memory improvement following treatment with Centella asiatica extract in adolescent rats. Brain Behav 2018; 8(9): e01093.
[http://dx.doi.org/10.1002/brb3.1093] [PMID: 30105867]
[19]
Noor Mohamad Zin NS, Hashim N, Samsulrizal N, Azmi NS. The protective effect of Azadirachta excelsa leaves extract and quercetin treatment on the learning and memory impairments in relation with insulin and amylin levels in the brain of streptozotocin-induced diabetic rats. J King Saud Univ Sci 2019; 31(3): 299-307.
[http://dx.doi.org/10.1016/j.jksus.2018.05.017]
[20]
Das A, Dikshit M, Nath C. Role of molecular isoforms of acetylcholinesterase in learning and memory functions. Pharmacol Biochem Behav 2005; 81(1): 89-99.
[http://dx.doi.org/10.1016/j.pbb.2005.02.006] [PMID: 15882896]
[21]
Reeta KH, Singh D, Gupta YK. Chronic treatment with taurine after intracerebroventricular streptozotocin injection improves cognitive dysfunction in rats by modulating oxidative stress, cholinergic functions and neuroinflammation. Neurochem Int 2017; 108: 146-56.
[http://dx.doi.org/10.1016/j.neuint.2017.03.006] [PMID: 28284975]
[22]
Arnett JJ, Žukauskienė R, Sugimura K. The new life stage of emerging adulthood at ages 18–29 years: Implications for mental health. Lancet Psychiatry 2014; 1(7): 569-76.
[http://dx.doi.org/10.1016/S2215-0366(14)00080-7] [PMID: 26361316]
[23]
Walker-Harding LR, Christie D, Joffe A, Lau JS, Neinstein L. Young adult health and well-being: A position statement of the society for adolescent health and medicine. J Adolesc Health 2017; 60(6): 758-9.
[http://dx.doi.org/10.1016/j.jadohealth.2017.03.021] [PMID: 28532650]
[24]
Beheshti F, Hosseini M, Vafaee F, Shafei MN, Soukhtanloo M. Feeding of nigella sativa during neonatal and juvenile growth improves learning and memory of rats. J Tradit Complement Med 2016; 6(2): 146-52.
[http://dx.doi.org/10.1016/j.jtcme.2014.11.039] [PMID: 27114937]
[25]
McDougall GJ Jr, Austin-Wells V, Zimmerman T. Utility of nutraceutical products marketed for cognitive and memory enhancement. J Holist Nurs 2005; 23(4): 415-33.
[http://dx.doi.org/10.1177/0898010105280097] [PMID: 16251490]
[26]
Alberini CM, Chen DY. Memory enhancement: Consolidation, reconsolidation and insulin-like growth factor 2. Trends Neurosci 2012; 35(5): 274-83.
[http://dx.doi.org/10.1016/j.tins.2011.12.007] [PMID: 22341662]
[27]
Tripathi G, Mishra S, Upadhyay P, Purohit S, Dubey GP. Ethnopharmacological importance of Centella asiatica with special reference to neuroprotective activity. Asia Pac J Med Toxicol 2015; 3(10): 49-53.
[28]
Hanifah M, Jufri M. Formulation and stability testing of nanoemulsion lotion containing Centella asiatica extract. J Young Pharm 2018; 10(4): 404.
[http://dx.doi.org/10.5530/jyp.2018.10.89]
[29]
Higaki A, Mogi M, Iwanami J, et al. Predicting outcome of Morris water maze test in vascular dementia mouse model with deep learning. PLoS One 2018; 13(2): e0191708.
[http://dx.doi.org/10.1371/journal.pone.0191708] [PMID: 29415035]
[30]
Jusril NA, Muhamad Juhari ANN, Abu Bakar SI, Md Saad WM, Adenan MI. Combining in silico and in vitro studies to evaluate the acetylcholinesterase inhibitory profile of different accessions and the biomarker triterpenes of Centella asiatica. Molecules 2020; 25(15): 3353.
[http://dx.doi.org/10.3390/molecules25153353] [PMID: 32721993]
[31]
Su J, Sripanidkulchai K, Wyss JM, Sripanidkulchai B. Curcuma comosa improves learning and memory function on ovariectomized rats in a long-term Morris water maze test. J Ethnopharmacol 2010; 130(1): 70-5.
[http://dx.doi.org/10.1016/j.jep.2010.04.012] [PMID: 20420894]
[32]
Hamezah HS, Durani LW, Ibrahim NF, et al. Volumetric changes in the aging rat brain and its impact on cognitive and locomotor functions. Exp Gerontol 2017; 99: 69-79.
[http://dx.doi.org/10.1016/j.exger.2017.09.008] [PMID: 28918364]
[33]
Nugroho A, Choi JS, Hong JP, Park HJ. Anti-acetylcholinesterase activity of the aglycones of phenolic glycosides isolated from Leonurus japonicus. Asian Pac J Trop Biomed 2017; 7(10): 849-54.
[http://dx.doi.org/10.1016/j.apjtb.2017.08.013]
[34]
Patil DN, Patil SA, Sistla S, Jadhav JP. Comparative biophysical characterization: A screening tool for acetylcholinesterase inhibitors. PLoS One 2019; 14(5): e0215291.
[http://dx.doi.org/10.1371/journal.pone.0215291] [PMID: 31150404]
[35]
Snyder J M, Gibson‐Corley K N, Radaelli E. Nervous system. Pathology of Genetically Engineered and Other Mutant Mice 2021; 462: 92.
[http://dx.doi.org/10.1002/9781119624608.ch21]
[36]
Iwata H, Yamamuro Y. Subregional expression of hippocampal glutamatergic and GABAergic genes in F344 rats with social isolation after weaning. Comp Med 2016; 66(1): 4-9.
[PMID: 26884404]
[37]
Mohamed D, Ahmed S. Donepezil improves histological and biochemical changes in the hippocampus of adult hypothyroid male rats. Egypt J Histol 2018; 41(4): 445-58.
[http://dx.doi.org/10.21608/ejh.2018.3807.1008]
[38]
Anwar J, Spanevello RM, Thomé G, et al. Effects of caffeic acid on behavioral parameters and on the activity of acetylcholinesterase in different tissues from adult rats. Pharmacol Biochem Behav 2012; 103(2): 386-94.
[http://dx.doi.org/10.1016/j.pbb.2012.09.006] [PMID: 22982740]
[39]
Amjad S, Umesalma S. Protective effect of Centella asiatica against aluminium-induced neurotoxicity in cerebral cortex, striatum, hypothalamus and hippocampus of rat brain-histopathological, and biochemical approach. J Mol Biomark Diagn 2015; 6(1): 1.
[40]
Dosumu O, Duru F, Osinubi A, Oremosu A, Noronha C. Influence of virgin coconut oil (VCNO) on oxidative stress, serum testosterone and gonadotropic hormones (FSH,LH) in chronic ethanol ingestion. Agric Biol J N Am 2010; 1(6): 1126-32.
[http://dx.doi.org/10.5251/abjna.2010.1.6.1126.1132]
[41]
Hafiz ZZ, Amin MAM, Johari James RM, Teh LK, Salleh MZ, Adenan MI. Inhibitory effects of raw-extract Centella asiatica (RECA) on acetylcholinesterase, inflammations, and oxidative stress activities via in vitro and in vivo. Molecules 2020; 25(4): 892.
[http://dx.doi.org/10.3390/molecules25040892] [PMID: 32079355]
[42]
Pangeni R, Sharma S, Mustafa G, Ali J, Baboota S. Vitamin E loaded resveratrol nanoemulsion for brain targeting for the treatment of Parkinson’s disease by reducing oxidative stress. Nanotechnology 2014; 25(48): 485102.
[http://dx.doi.org/10.1088/0957-4484/25/48/485102] [PMID: 25392203]
[43]
Jusril NA, Abu Bakar SI, Khalil KA, Md Saad WM, Wen NK, Adenan MI. Development and optimization of nanoemulsion from ethanolic extract of Centella asiatica (NanoSECA) using D-Optimal mixture design to improve blood-brain barrier permeability. Evid Based Complement Alternat Med 2022; 2022: 1-18.
[http://dx.doi.org/10.1155/2022/3483511] [PMID: 35295926]

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