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Endocrine, Metabolic & Immune Disorders - Drug Targets

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ISSN (Print): 1871-5303
ISSN (Online): 2212-3873

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Research Article

Periostin Acts as a Bridge between Gestational Diabetes Mellitus (GDM) and Chronic Inflammation to Modulate Insulin Resistance by Modulating PPARα/NF-κB/TNF-α Signaling Pathway

Author(s): Qun Ji, Xinying Li, Yan Wang, Haiwei Liu, Kaining Chen, Huibiao Quan, Huachuan Zhang and Jianmin Ran*

Volume 23, Issue 13, 2023

Published on: 06 July, 2023

Page: [1649 - 1659] Pages: 11

DOI: 10.2174/1871530323666230427104724

Price: $65

Abstract

Introduction: Gestational diabetes mellitus (GDM) is considered an imbalance of glucose metabolism and insulin resistance during pregnancy.

Aims/Objective: To evaluate the levels of periostin (POSTN) in patients with GDM and investigate the association between POSTN and GDM.

Materials and Methods: A total of 30 pregnant women (NC group) and 30 pregnant women with GDM (GDM group) were involved. The GDM mouse model was established by intraperitoneally injecting streptozotocin. The oral glucose tolerance test (OGTT), insulin, and insulin resistance indices were tested. An immunohistochemical and Western blot assay was conducted to determine the expression of POSTN, PPARα, TNF-α, and NF-κB. HE staining was performed to evaluate inflammation in the placental tissues of women with GDM and GDM mice. POSTN-siRNA was transfected into glucose-pretreated HTR8 cells, and pAdEasy-m-POSTN shRNA was infected in GDM mice. The RT-PCR assay determined the gene transcription of POSTN, TNF-α, NF-κB, and PPARα.

Results: Pregnantwomen in theGDMgroup demonstrated significantly higherOGTT (p < 0.05), insulin levels (p < 0.05) and insulin resistance (p < 0.05) compared to those of the NC group. The serum levels of POSTN in pregnantwomen of theGDMgroup were significantly higher than that of theNC group (p < 0.05). The obvious inflammation was activated in pregnant women in the GDMgroup. POSTN-siRNAsignificantly enhanced the cell viability of glucose-treated HTR8 cells compared to that without glucose treatment (p < 0.05). POSTNsiRNA (pAdEasy-m-POSTN shRNA) markedly reduced the glucose level of glucose-treated HTR8 cells (GDM mice) compared to that without treatment (p < 0.05). POSTN-siRNA (pAdEasy-m-POSTN shRNA) promoted PPARα gene transcription (p < 0.05) and inhibited NF-κB/TNF-α gene transcription (p < 0.05) in glucose-treated HTR8 cells (GDMmice) compared to thosewithout treatment. POSTN-siRNAmodulated NF- κB/TNF-α pathway mediated inflammation by regulating PPARα in HTR8 cells and GDMmice. PPARα participated in POSTN-associated inflammation. pAdEasy-m-POSTN shRNA inhibited T-CHO/TG levels in GDM mice compared to those without treatment (p < 0.05). All the effects of POSTN-siRNA (pAdEasy-m- POSTN shRNA) were obviously blocked by PPARα inhibitor treatment.

Conclusion: POSTN levels were significantly higher in pregnant women with GDM and were associated with chronic inflammation and PPARα expression. POSTN may act as a bridge between GDM and chronic inflammation to modulate insulin resistance by modulating PPARα/NF-κB/TNF-α signaling pathway.

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[1]
Ahmadi-Motamayel F, Fathi S, Goodarzi MT, Borzouei S, Poorolajal J, Barakian Y. Comparison of salivary antioxidants and oxidative stress status in gestational diabetes mellitus and healthy pregnant women. Endocr Metab Immune Disord Drug Targets 2021; 21(8): 1485-90.
[http://dx.doi.org/10.2174/1568026620666201022151059] [PMID: 33092517]
[2]
Ismail NA, Mohamed I. N.A.; Bador, K.M. Vitamin D in gestational diabetes mellitus and its association with hyperglycaemia, insulin sensitivity and other factors. J Obstet Gynaecol 2021; 41(6): 899-903.
[http://dx.doi.org/10.1080/01443615.2020.1820462] [PMID: 33962550]
[3]
Schiavone M, Putoto G, Laterza F, Pizzol D. Gestational diabetes: An overview with attention for developing countries. Endocr Regul 2016; 50(2): 62-71.
[http://dx.doi.org/10.1515/enr-2016-0010] [PMID: 27560638]
[4]
Pantham P, Aye ILMH, Powell TL. Inflammation in maternal obesity and gestational diabetes mellitus. Placenta 2015; 36(7): 709-15.
[http://dx.doi.org/10.1016/j.placenta.2015.04.006] [PMID: 25972077]
[5]
Kim SY, Sappenfield W, Sharma AJ, et al. Racial/ethnic differences in the prevalence of gestational diabetes mellitus and maternal overweight and obesity, by Nativity, Florida, 2004-2007. Obesity (Silver Spring) 2013; 21(1): E33-40.
[http://dx.doi.org/10.1002/oby.20025] [PMID: 23404915]
[6]
Kim C, Newton KM, Knopp RH. Gestational diabetes and the incidence of type 2 diabetes: A systematic review. Diabetes Care 2002; 25(10): 1862-8.
[http://dx.doi.org/10.2337/diacare.25.10.1862] [PMID: 12351492]
[7]
Lambrinoudaki I, Vlachou SA, Creatsas G. Genetics in gestational diabetes mellitus: Association with incidence, severity, pregnancy outcome and response to treatment. Curr Diabetes Rev 2010; 6(6): 393-9.
[http://dx.doi.org/10.2174/157339910793499155] [PMID: 20879971]
[8]
Świrska, J.; Zwolak, A.; Dudzińska, M.; Matyjaszek-Matuszek, B.; Paszkowski, T. Gestational diabetes mellitus — literature review on selected cytokines and hormones of confirmed or possible role in its pathogenesis. Ginekol Pol 2018; 89(9): 522-7.
[http://dx.doi.org/10.5603/GP.a2018.0089] [PMID: 30318581]
[9]
Lawrence T. The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harb Perspect Biol 2009; 1(6): a001651.
[http://dx.doi.org/10.1101/cshperspect.a001651] [PMID: 20457564]
[10]
Li Y, Long D, Liu J, et al. Gestational diabetes mellitus in women increased the risk of neonatal infection via inflammation and autophagy in the placenta. Medicine 2020; 99(40): e22152.
[http://dx.doi.org/10.1097/MD.0000000000022152] [PMID: 33019392]
[11]
Tak PP. Firestein, G.S. NF-κB: A key role in inflammatory diseases. J Clin Invest 2001; 107(1): 7-11.
[http://dx.doi.org/10.1172/JCI11830] [PMID: 11134171]
[12]
Luo Y, Qu H, Wang H, et al. Plasma periostin levels are increased in chinese subjects with obesity and type 2 diabetes and are positively correlated with glucose and lipid parameters. Mediators Inflamm 2016; 2016: 1-6.
[http://dx.doi.org/10.1155/2016/6423637] [PMID: 27313402]
[13]
Conway SJ, Izuhara K, Kudo Y, et al. The role of periostin in tissue remodeling across health and disease. Cell Mol Life Sci 2014; 71(7): 1279-88.
[http://dx.doi.org/10.1007/s00018-013-1494-y]
[14]
Lu Y, Liu X, Jiao Y, et al. Periostin promotes liver steatosis and hypertriglyceridemia through downregulation of PPARα. J Clin Invest 2014; 124(8): 3501-13.
[http://dx.doi.org/10.1172/JCI74438]
[15]
Li J, Niu X, Si Q, et al. Plasma periostin as a biomarker of osteoporosis in postmenopausal women with type 2 diabetes. J Bone Miner Metab 2021; 39(4): 631-8.
[http://dx.doi.org/10.1007/s00774-020-01200-3] [PMID: 33566208]
[16]
Ding Y, Ge Q, Qu H, et al. Increased serum periostin concentrations are associated with the presence of diabetic retinopathy in patients with type 2 diabetes mellitus. J Endocrinol Invest 2018; 41(8): 937-45.
[http://dx.doi.org/10.1007/s40618-017-0820-x] [PMID: 29349642]
[17]
Guan J, Liu WQ, Xing MQ, et al. Elevated expression of periostin in diabetic cardiomyopathy and the effect of valsartan. BMC Cardiovasc Disord 2015; 15(1): 90.
[http://dx.doi.org/10.1186/s12872-015-0084-3] [PMID: 26281830]
[18]
Sun XJ, Ma WQ, Zhu Y, Liu NF. POSTN promotes diabetic vascular calcification by interfering with autophagic flux. Cell Signal 2021; 83: 109983.
[http://dx.doi.org/10.1016/j.cellsig.2021.109983] [PMID: 33744420]
[19]
Diagnosis and classification of diabetes mellitus. Diabetes Care 2014; 37 (Suppl. 1): S81-90.
[http://dx.doi.org/10.2337/dc14-S081] [PMID: 24357215]
[20]
Chen SH, Liu XN, Peng Y. MicroRNA‐351 eases insulin resistance and liver gluconeogenesis via the PI3K/AKT pathway by inhibiting FLOT2 in mice of gestational diabetes mellitus. J Cell Mol Med 2019; 23(9): 5895-906.
[http://dx.doi.org/10.1111/jcmm.14079] [PMID: 31287224]
[21]
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)). Method Methods 2001; 25(4): 402-8.
[http://dx.doi.org/10.1006/meth.2001.1262] [PMID: 11846609]
[22]
Zhang Z, Li J, Hu T, Xu C, Xie N, Chen D. Interventional effect of dietary fiber on blood glucose and pregnancy outcomes in patients with gestational diabetes mellitus. Zhejiang Da Xue Xue Bao Yi Xue Ban 2021; 50(3): 305-12.
[http://dx.doi.org/10.3724/zdxbyxb-2021-0115]
[23]
Homko C, Sivan E, Chen X, Reece EA, Boden G. Insulin secretion during and after pregnancy in patients with gestational diabetes mellitus. J Clin Endocrinol Metab 2001; 86(2): 568-73.
[http://dx.doi.org/10.1210/jc.86.2.568] [PMID: 11158010]
[24]
Embaby H, Elsayed E, Fawzy M. Insulin sensitivity and plasma glucose response to aerobic exercise in pregnant women at risk for gestational diabetes mellitus. Ethiop J Health Sci 2016; 26(5): 409-14.
[http://dx.doi.org/10.4314/ejhs.v26i5.2] [PMID: 28446846]
[25]
Merle B, Garnero P. The multiple facets of periostin in bone metabolism. Osteoporos Int 2012; 23(4): 1199-212.
[http://dx.doi.org/10.1007/s00198-011-1892-7] [PMID: 22310955]
[26]
Chen X, Huo L, Ren L, et al. Polycystic ovary syndrome is associated with elevated periostin levels. Exp Clin Endocrinol Diabetes 2019; 127(9): 571-7.
[http://dx.doi.org/10.1055/a-0752-0061] [PMID: 30372763]
[27]
Park MH, Kim DH, Kim MJ, et al. Effects of MHY908, a new synthetic pparα/γ dual agonist, on inflammatory responses and insulin resistance in aged rats. J Gerontol A Biol Sci Med Sci 2016; 71(3): 300-9.
[http://dx.doi.org/10.1093/gerona/glv043] [PMID: 26219845]
[28]
Jung TW, Kim HC, Abd El-Aty AM, Jeong JH. Protectin DX ameliorates palmitate- or high-fat diet-induced insulin resistance and inflammation through an AMPK-PPARα-dependent pathway in mice. Sci Rep 2017; 7(1): 1397.
[http://dx.doi.org/10.1038/s41598-017-01603-9] [PMID: 28469249]
[29]
Atègbo JM, Grissa O, Yessoufou A, et al. Modulation of adipokines and cytokines in gestational diabetes and macrosomia. J Clin Endocrinol Metab 2006; 91(10): 4137-43.
[http://dx.doi.org/10.1210/jc.2006-0980] [PMID: 16849405]

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