Login Register Cart 0
Generic placeholder image

Pharmaceutical Nanotechnology

Editor-in-Chief

ISSN (Print): 2211-7385
ISSN (Online): 2211-7393

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Antimicrobial Activity of the Nanoparticle Form of Greens (Lemon, Black Seeds or Flax) with Silver on Drug-resistant Human Pathogens

Author(s): Suad Yousif Aldorkee and Ali Abdul Hussein S. AL-Janabi*

Volume 11, Issue 4, 2023

Published on: 05 May, 2023

Page: [339 - 343] Pages: 5

DOI: 10.2174/2211738511666230329122924

Price: $65

conference banner
Abstract

Background: Synthesizing a green source that has antimicrobial activity in nanoparticles is a novel and exciting approach to pharmaceutical science with promising results.

Objective: Green-silver nanoparticles (G-AgNPs) were evaluated for their antimicrobial action on drugresistant pathogens.

Methods: Lemon, black seeds, and flax were selected as green sources to synthesize nanoparticles formed with silver. Physical and chemical characteristics of these preparations were identified. The antimicrobial activities of the prepared compounds against drug-resistant clinical isolates of seven bacteria and five fungi were identified by disk diffusion and dilution methods.

Results: The nanoparticle characteristics were confirmed by physical and chemical measurements. Lemon extract with silver nanoparticles (L-AgNP) showed more antimicrobial action, particularly on Gram-positive bacteria and Candida albicans. Silver nanoparticles with black seeds (B-AgNP) and flax (F-AgNP) had only antibacterial effects on a single bacterium (Enterobacter cloacae). Escherichia coli, Staphylococcus aureus, and two fungi, Candida glabrata and Candida utilis, showed resistance to all nanoparticles from plants.

Conclusion: Lemon with silver nanoparticle is an effective plant product for use against various drugresistant species of human pathogens. Further pharmaceutical studies are required to verify the suitability of this form of the drug for human use. Another plant is recommended for testing against the most resistant strains of pathogens

« Previous Next »
Graphical Abstract

[1]
Alvi GB, Iqbal MS, Ghaith MMS, Haseeb A, Ahmed B, Qadir MI. Biogenic selenium nanoparticles (SeNPs) from citrus fruit have anti-bacterial activities. Sci Rep 2021; 11(1): 4811.
[http://dx.doi.org/10.1038/s41598-021-84099-8] [PMID: 33637796]
[2]
Vankar PS, Shukla D. Biosynthesis of silver nanoparticles using lemon leaves extract and its application for antimicrobial finish on fabric. Appl Nanosci 2012; 2(2): 163-8.
[http://dx.doi.org/10.1007/s13204-011-0051-y]
[3]
Mosae SP, Antonyraj CA, Babu R, Dakhsinamurthy A, Manikandan N, Palanivel A. Green synthesis and anti-microbial activity of monodispersed silver nanoparticles synthesized using lemon extract. Synth React Inorg Met-Org Nano-Met Chem 2016; 46(2): 291-4.
[http://dx.doi.org/10.1080/15533174.2014.971810]
[4]
Chen Q, Ma J, Hu Y, Fei P. Multifunctional antibacterial films with silver nanoparticles reduced in situ by lemon juice. Food Chem 2021; 365: 130517.
[http://dx.doi.org/10.1016/j.foodchem.2021.130517] [PMID: 34252625]
[5]
Dhinek A, Vanitha S. Synthesis and characterization of silver nanoparticle from Citrus limon leaves and its antibacterial activity. IJSR 2018; 7: 8-10.
[6]
Shet AR, Ghose P, Patil L, Hombalimath V. A preliminary study on green synthesis and antibacterial activity of silver nanoparticles. Int J Curr Biotechnol 2015; 3: 1-6.
[7]
Chahardoli A, Karimi N, Fattahi A. Biosynthesis, characterization, antimicrobial and cytotoxic effects of silver nanoparticles using Nigella arvensis seed extract. Iran J Pharm Res 2017; 16(3): 1167-75.
[PMID: 29201104]
[8]
Almatroudi A, Khadri H, Azam M, et al. Antibacterial, antibiofilm and anticancer activity of biologically synthesized silver nanoparticles using seed extract of Nigella sativa. Processes (Basel) 2020; 8(4): 388.
[http://dx.doi.org/10.3390/pr8040388]
[9]
Sanusi JF, Ishola QA, Alao SO, Sakariyau AO. Characterization and antibacterial activities of silver nanoparticles synthesized using black seed (Nigella sativa). AJBAR 2022; 1: 65-74.
[http://dx.doi.org/10.55639/607gpx]
[10]
Ranjan P, Das MP, Kumar MS, et al. Green synthesis and characterization of silver nanoparticles from Nigella sativa and its application against UTI causing bacteria. J Acad Ind Res 2013; 2: 45-9.
[11]
Ali T, Hussain F, Naeem M, Khan A, Al-Harrasi A. AI-Harrasi A. Nanotechnology approach for exploring the enhanced bioactivities and biochemical characterization of freshly prepared Nigella sativa L. nanosuspensions and their phytochemical profile. Front Bioeng Biotechnol 2022; 10: 888177.
[http://dx.doi.org/10.3389/fbioe.2022.888177] [PMID: 35656198]
[12]
Nallamuthu I, Parthasarathi A, Khanum F. Thymoquinone-loaded PLGA nanoparticles: Antioxidant and anti-microbial properties. Int Curr Pharm J 2013; 2(12): 202-7.
[http://dx.doi.org/10.3329/icpj.v2i12.17017]
[13]
Ghaedi M, Yousefi-Nejad M, Safarpoor M, et al. Investigation of phytochemical and antimicrobial properties of Linum usitatissimum in presence of ZnO/Zn(OH) 2 nanoparticles and extraction of euphol from Euphorbia microsciadia. Desalination Water Treat 2016; 57(43): 20597-607.
[http://dx.doi.org/10.1080/19443994.2015.1108877]
[14]
Anjum S, Abbasi BH. Thidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of Linum usitatissimum L. Int J Nanomedicine 2016; 11: 715-28.
[PMID: 26955271]
[15]
Bhuvaneshwari P, Vinoba P, Prabhu N, Singh MVP, Rajamehala M, Archana A. A newly synthesized silver nanoparticles mediated phytomedicine from Linum usitatissimum for dental caries. J Pharm Res Int 2021; 33: 150-60.
[http://dx.doi.org/10.9734/jpri/2021/v33i55B33859]
[16]
Rajeshkumar S. Citrus lemon juice mediated preparation of AgNOs/chitosan-based bionanocomposities and its antimicrobial and antioxidant activity. J Nanomater 2021; 2021: 1-10.
[http://dx.doi.org/10.1155/2021/7527250]
[17]
Samreen FG, Muzaffar R, Nawaz M, Gul S, Basra MA. Synthesis, characterization and anti-microbial activity of Citrus limon mediated nanoparticles. Preprints 2018; 2018; 110417.
[http://dx.doi.org/10.20944/preprints201811.0417.v1]
[18]
Nasr HA, Nassar OM, El-Sayed MH, Kobisi AA. Characterization and antimicrobial activity of lemon peel mediated green synthesis of silver nanoparticles. Int J Biochem 2019; 12(2): 56-63.
[http://dx.doi.org/10.26577/ijbch-2019-i2-7]
[19]
Khane Y, Benouis K, Albukhaty S, et al. Green synthesis of silver nanoparticles using aqueous Citrus limon Zest extract: characterization and evaluation of their antioxidant and antimicrobial properties. Nanomaterials (Basel) 2022; 12(12): 2013.
[http://dx.doi.org/10.3390/nano12122013] [PMID: 35745352]
[20]
Ghaedi M, Yousefi-Nejad M, Safarpoor M, et al. Synthesis of CuS nanoparticles and evaluation of its antimicrobial properties in combination with Linum usitatissimum root and shoot extract. Desalination Water Treat 2016; 57(51): 24456-66.
[http://dx.doi.org/10.1080/19443994.2016.1138896]
[21]
Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing: Approved standard- 30th edition. Document M100. Wayne. Pennsylvania. 2020.
[22]
Clinical and Laboratory Standards Institute (CLSI). Method for antifungal disk diffusion susceptibility testing of yeasts: Approved standard-3rd edition. Document M44. Wayne. Pennsylvania. 2018.
[23]
Clinical and Laboratory Standards Institute (CLSI). Performance standards for antifungal susceptibility testing of yeasts: Approved standard-2nd edition. Document M60. Wayne. Pennsylvania. 2020.

Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy