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Current Drug Discovery Technologies

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ISSN (Print): 1570-1638
ISSN (Online): 1875-6220

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Research Article

Evaluation of Wound Healing Potential of Root Bark Extract of Berberis aristata and Molecular Docking Analysis of Berberis Phytoconstituents

Author(s): Alankar Shrivastav*, Arun Kumar Mishra and Ashessh Kumar Gupta

Volume 20, Issue 3, 2023

Published on: 22 March, 2023

Article ID: e210223213867 Pages: 17

DOI: 10.2174/1570163820666230221154851

Price: $65

Abstract

Introduction: The root bark of Berberis aristata has been utilized by indigenous peoples for wound treatment for centuries. The mature root barks are crushed into a paste and applied to the wound's surface.

Objective: The focus of this research is to analyse the wound healing activities of an ethanolic extract of Berberis aristata, as well as to use molecular docking to establish the likely mechanism of the potent phytochemical. There is no scientific evidence to support the usage of root bark extract of Berberis aristata.

Methods: The Herbal ointment, which comprises (1%, 2%, and 4% w/w) ethanolic extract of root bark, was developed to test the wound healing ability of incision and excision wounds, and the molecular mechanism was established using Auto-Dock software.

Results: Epithelization stage, wound index, % wound contraction area, hydroxyproline content, DNA estimate, and histopathological assessments were performed on the incision wound model. Tensile strength was assessed in an excision wound model. TLC was used to identify the samples after successive extractions with different solvents based on polarity.

Conclusion: Berberine and tetrahydropalmatine were major active phytoconstituent found in root barks of Berberis aristata as secondary metabolites. Animals treated with 4% w/w formulation demonstrated considerable wound contraction, epithelization time, and wound index in the excision model. In contrast, to control and standardize the concentrations of hydroxyproline, total amino acids, and DNA in recovering tissue were higher. At 4% w/w extract formulation, the parameters studied indicated a substantial result. Berberine and tetrahydropalmatine, active metabolites which are present in the ethanolic extract of Berberis aristata, were found to be responsible for wound healing. Based on ligand interactions, the findings verified Berberis aristata ethnomedicinal claim in a wound healing capacity.

Graphical Abstract

[1]
Gurib-Fakim A. Medicinal plants: Traditions of yesterday and drugs of tomorrow. Mol Aspects Med 2006; 27(1): 1-93.
[http://dx.doi.org/10.1016/j.mam.2005.07.008] [PMID: 16105678]
[2]
Sala A, Recio MC, Giner RM, et al. Anti-inflammatory and antioxidant properties of Helichrysum italicum. J Pharm Pharmacol 2010; 54(3): 365-71.
[http://dx.doi.org/10.1211/0022357021778600] [PMID: 11902802]
[3]
Van WBE, Wink M. Medicinal Plants Of The World. CABI 2018.
[4]
Balunas MJ, Kinghorn AD. Drug discovery from medicinal plants. Life Sci 2005; 78(5): 431-41.
[http://dx.doi.org/10.1016/j.lfs.2005.09.012] [PMID: 16198377]
[5]
Tshikalange TE, Meyer JJM, Hussein AA. Antimicrobial activity, toxicity and the isolation of a bioactive compound from plants used to treat sexually transmitted diseases. J Ethnopharmacol 2005; 96(3): 515-9.
[http://dx.doi.org/10.1016/j.jep.2004.09.057] [PMID: 15619572]
[6]
Bhalerao SA, Kelkar TS. Phytochemical and pharmacological potential of Tridax procumbens Linn. Int J Adv Biol Biomed Res 2012; 2(3): 392-5.
[7]
Udupa S, Udupa A, Kulkarni D. Influence of Tridax procumbens on lysyl oxidase activity and wound healing. Planta Med 1991; 57(4): 325-7.
[http://dx.doi.org/10.1055/s-2006-960108] [PMID: 1685591]
[8]
Yaduvanshi B, Mathur R, Mathur SR, Velpandian T. Evaluation of wound healing potential of topical formulation of leaf juice of tridax procumbens L. in mice. Indian J Pharm Sci 2011; 73(3): 303-6.
[PMID: 22457556]
[9]
Sharma P. Dravya Guna-Vigyan (AubhidAusadh –Dravya). Varanasi, India: Chaukhambha Bhartiya Academy 2001.
[10]
Torrance SJ, Wiedhopf RM, Cole JR, et al. Antitumor agents from Jatropha macrorhiza (Euphorbiaceae). II. Isolation and characterization of jatrophatrione. J Org Chem 1976; 41(10): 1855-7.
[http://dx.doi.org/10.1021/jo00872a038] [PMID: 1262992]
[11]
Al-Mohammadi AR, Ibrahim RA, Moustafa AH, Ismaiel AA, Abou Zeid A, Enan G. Chemical constitution and antimicrobial activity of kefir fermented beverage. Molecules 2021; 26(9): 2635.
[http://dx.doi.org/10.3390/molecules26092635] [PMID: 33946451]
[12]
Yu-Peng LI, Kang YANG, Hui MENG, Tao SHEN, Hua ZHANG. Polyhydroxylated eudesmane sesquiterpenoids and sesquiterpenoid glucoside from the flower buds of Tussilago farfara. Chin J Nat Med 2021; 19: 1-8.
[13]
Pradhan D, Kar DM, Ghosh G. Bioassay-guided fractionation of anti-microbial principle from ethanolic extract of Tinospora cordifolia and its characterization. Res J Pharmacy Technol 2020; 13(3): 1415-22.
[http://dx.doi.org/10.5958/0974-360X.2020.00259.0]
[14]
Tilvi S, Parvatkar R, Singh KS, Devi P. Chemical investigation of marine‐derived fungus Aspergillus flavipes for potential anti‐inflammatory agents. Chem Biodivers 2021; 18(2): e2000956.
[http://dx.doi.org/10.1002/cbdv.202000956] [PMID: 33533162]
[15]
OECD. Guidelines for testing of chemicals. Acute Dermal Toxicity 1987; 402: 1-7.
[16]
Nagar HK, Srivastava AK, Srivastava R, Kurmi ML, Chandel HS, Ranawat MS. Pharmacological investigation of the wound healing activity of Cestrum nocturnum (L.) ointment in Wistar albino rats. J Pharm 2016; 2016: 1-8.
[http://dx.doi.org/10.1155/2016/9249040] [PMID: 27018126]
[17]
Özbilgin S. Acıkara ÖB, Akkol EK, Süntar I, Keleş H, İşcan GS. In vivo wound-healing activity of Euphorbia characias subsp wulfenii: Isolation and quantification of quercetin glycosides as bioactive compounds. J Ethnopharmacol 2018; 224: 400-8.
[http://dx.doi.org/10.1016/j.jep.2018.06.015] [PMID: 29920357]
[18]
Patil MVK, Kandhare AD, Bhise SD. Pharmacological evaluation of ethanolic extract of Daucus carota Linn root formulated cream on wound healing using excision and incision wound model. Asian Pac J Trop Biomed 2012; 2(2): S646-55.
[http://dx.doi.org/10.1016/S2221-1691(12)60290-1]
[19]
Neuman RE, Logan MA. The determination of hydroxyproline. J Biol Chem 1950; 184(1): 299-306.
[http://dx.doi.org/10.1016/S0021-9258(19)51149-8] [PMID: 15421999]
[20]
Lowry O, Rosebrough N, Farr AL, Randall R. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193(1): 265-75.
[http://dx.doi.org/10.1016/S0021-9258(19)52451-6] [PMID: 14907713]
[21]
Burton K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 1956; 62(2): 315-23.
[http://dx.doi.org/10.1042/bj0620315] [PMID: 13293190]
[22]
Saha K, Mukherjee PK, Das J, Pal M, Saha BP. Wound healing activity of Leucas lavandulaefolia Rees. J Ethnopharmacol 1997; 56(2): 139-44.
[http://dx.doi.org/10.1016/S0378-8741(97)01522-5] [PMID: 9174975]
[23]
Nagappa AN, Cheriyan B. Wound healing activity of the aqueous extract of Thespesia populnea fruit. Fitoterapia 2001; 72(5): 503-6.
[http://dx.doi.org/10.1016/S0367-326X(01)00275-1] [PMID: 11429243]
[24]
Tümen İ Akkol EK, Taştan H, Süntar I, Kurtca M. Research on the antioxidant, wound healing, and anti-inflammatory activities and the phytochemical composition of maritime pine (Pinus pinaster Ait). J Ethnopharmacol 2018; 211: 235-46.
[http://dx.doi.org/10.1016/j.jep.2017.09.009] [PMID: 28917972]
[25]
Mukherjee PK, Verpoorte R, Suresh B. Evaluation of in-vivo wound healing activity of Hypericum patulum (Family: Hypericaceae) leaf extract on different wound model in rats. J Ethnopharmacol 2000; 70(3): 315-21.
[http://dx.doi.org/10.1016/S0378-8741(99)00172-5] [PMID: 10837993]
[26]
Akkol EK, Koca U. , Peşin I, Yılmazer D, Toker G, Yeşilada E. Exploring the wound healing activity of Arnebia densiflora (Nordm.) Ledeb. by in vivo models. J Ethnopharmacol 2009; 124(1): 137-41.
[http://dx.doi.org/10.1016/j.jep.2009.03.019] [PMID: 19500923]
[27]
Morris GM, Lim-Wilby M. Molecular docking Molecular modeling of proteins. Humana Press 2008; pp. 365-82.
[http://dx.doi.org/10.1007/978-1-59745-177-2_19]
[28]
Čechová P, Kubala M. Comparison of three nature inspired molecular docking algorithms. Int J Bio-inspired Comput 2021; 17(1): 34-41.
[http://dx.doi.org/10.1504/IJBIC.2021.113362]
[29]
Meng XY, Zhang HX, Mezei M, Cui M. Molecular docking: A powerful approach for structure-based drug discovery. Curr Computeraided Drug Des 2011; 7(2): 146-57.
[http://dx.doi.org/10.2174/157340911795677602] [PMID: 21534921]
[30]
Iwalewa EO, Suleiman MM, Mdee LK, Eloff JN. Antifungal and antibacterial activities of different extracts of Harungana madagascariensis stem bark. Pharm Biol 2009; 47(9): 878-85.
[http://dx.doi.org/10.1080/13880200903029316]
[31]
Malheiro JF, Maillard JY, Borges F, Simões M. Biocide potentiation using cinnamic phytochemicals and derivatives. Molecules 2019; 24(21): 3918.
[http://dx.doi.org/10.3390/molecules24213918] [PMID: 31671687]
[32]
Savadi S, Vazifedoost M, Didar Z, Nematshahi MM, Jahed E. Phytochemical analysis and antimicrobial/antioxidant activity of Cynodon dactylon (L.) Pers. rhizome methanolic extract. J Food Qual 2020; 2020: 1-10.
[http://dx.doi.org/10.1155/2020/5946541]
[33]
Etame RE, Mouokeu RS, Pouaha CLC, et al. Effect of fractioning on antibacterial activity of enantia chlorantha oliver (Annonaceae) methanol extract and mode of action. Evid-Based Complementary Altern Med 2018; 1-8.
[34]
Azwanida NN. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants 2015; 4(196): 2167-0412.
[35]
Pacheco-Palencia LA, Duncan CE, Talcott ST. Phytochemical composition and thermal stability of two commercial açai species, Euterpe oleracea and Euterpe precatoria. Food Chem 2009; 115(4): 1199-205.
[http://dx.doi.org/10.1016/j.foodchem.2009.01.034]
[36]
Abdullah S, Shaari AR, Azimi A. Effect of drying methods on metabolites composition of misai kucing (Orthosiphon stamineus) leaves. APCBEE Procedia 2012; 2: 178-82.
[http://dx.doi.org/10.1016/j.apcbee.2012.06.032]
[37]
Süntar I, Akkol EK. Keleş H, Oktem A, Başer KHC, Yeşilada E. A novel wound healing ointment: A formulation of Hypericum perforatum oil and sage and oregano essential oils based on traditional Turkish knowledge. J Ethnopharmacol 2011; 134(1): 89-96.
[http://dx.doi.org/10.1016/j.jep.2010.11.061] [PMID: 21130859]
[38]
Ghosh P, Kandhare AD, Gauba D, Raygude KS, Bodhankar SL. Determination of efficacy, adverse drug reactions and cost effectiveness of three triple drug regimens for the treatment of Helicobacter pylori infected acid peptic disease patients. Asian Pac J Trop Dis 2012; 2: S783-9.
[http://dx.doi.org/10.1016/S2222-1808(12)60265-5]
[39]
Altiok D, Altiok E, Tihminlioglu F. Physical, antibacterial and antioxidant properties of chitosan films incorporated with thyme oil for potential wound healing applications. J Mater Sci Mater Med 2010; 21(7): 2227-36.
[http://dx.doi.org/10.1007/s10856-010-4065-x] [PMID: 20372985]
[40]
Patil MVK, Kandhare AD, Bhise SD. Effect of aqueous extract of Cucumis sativus Linn. fruit in ulcerative colitis in laboratory animals. Asian Pac J Trop Biomed 2012; 2(2): S962-9.
[http://dx.doi.org/10.1016/S2221-1691(12)60344-X]
[41]
Saarialho-Kere UK, Kovacs SO, Pentland AP, Olerud JE, Welgus HG, Parks WC. Cell-matrix interactions modulate interstitial collagenase expression by human keratinocytes actively involved in wound healing. J Clin Invest 1993; 92(6): 2858-66.
[http://dx.doi.org/10.1172/JCI116906] [PMID: 8254040]

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