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Current Radiopharmaceuticals

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ISSN (Print): 1874-4710
ISSN (Online): 1874-4729

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Research Article

Investigation of Bioactivity of Estragole Isolated from Basil Plant on Brain Cancer Cell Lines Using Nuclear Method

Author(s): Ugur Avcibasi*, Mouhaman Toukour Dewa, Kadriye Busra Karatay, Ayfer Yurt Kilcar and Fazilet Zumrut Biber Muftuler

Volume 16, Issue 2, 2023

Published on: 20 January, 2023

Page: [140 - 150] Pages: 11

DOI: 10.2174/1874471016666230110144021

Price: $65

Abstract

Background: In recent years, there has been a significant increase in studies investigating the potential use of plant-origin products in the treatment and diagnosis of different types of cancer.

Methods: In this study, Estragole (EST) was isolated from basil leaves via ethanolic extraction using an 80% ethanol concentration. The isolation process was performed using the High Performance Liquid Chromatography (HPLC) method. The EST isolated from the basil plant was radiolabeled with 131I using the iodogen method. Quality control studies of the radiolabeled EST (131IEST) were carried out by using Thin Layer Radio Chromatography (TLRC). Next, in vitro cell, culture studies were done to investigate the bio-affinity of plant-originated EST labeled with 131I on human medulloblastoma (DAOY) and human glioblastoma-astrocytoma (U-87 MG) cell lines. Finally, the cytotoxicity of EST was determined, and cell uptake of 131I-EST was investigated on cancer cell lines by incorporation studies.

Results: As a result of these studies, it has been shown that 131I-EST has a significant uptake on the brain cells.

Conclusion: This result is very satisfying, and it has encouraged us to do in vivo studies for the molecule in the future.

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[1]
Bray, F.; Ferlay, J.; Soerjomataram, I.; Siegel, R.L.; Torre, L.A.; Jemal, A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J. Clin., 2018, 68(6), 394-424.
[http://dx.doi.org/10.3322/caac.21492] [PMID: 30207593]
[2]
Wang, Z.; Zhang, H.; Shi, M.; Yu, Y.; Wang, H.; Cao, W.M.; Zhao, Y.; Zhang, H. TAK1 inhibitor NG25 enhances doxorubicin-mediated apoptosis in breast cancer cells. Sci. Rep., 2016, 6(1), 32737.
[http://dx.doi.org/10.1038/srep32737] [PMID: 27599572]
[3]
Weingart, S.N.; Zhang, L.; Sweeney, M.; Hassett, M. Chemotherapy medication errors. Lancet Oncol., 2018, 19(4), e191-e199.
[http://dx.doi.org/10.1016/S1470-2045(18)30094-9] [PMID: 29611527]
[4]
Majolo, F.; de Oliveira Becker Delwing, L.K.; Marmitt, D.J.; Bustamante-Filho, I.C.; Goettert, M.I. Medicinal plants and bioactive natural compounds for cancer treatment: Important advances for drug discovery. Phytochem. Lett., 2019, 31, 196-207.
[http://dx.doi.org/10.1016/j.phytol.2019.04.003]
[5]
Bapat, K.; Chintalwar, G.J.; Pandey, U.; Thakur, V.S.; Sarma, H.D.; Samuel, G.; Pillai, M.R.A.; Chattopadhyay, S.; Venkatesh, M. Preparation and in vitro evaluation of radioiodinated bakuchiol as an anti tumor agent. Appl. Radiat. Isot., 2005, 62(3), 389-393.
[http://dx.doi.org/10.1016/j.apradiso.2004.07.007] [PMID: 15607914]
[6]
Biber Muftuler, F.Z.; Yurt Kilcar, A.; Ünak, P. A perspective on plant origin radiolabeled compounds, their biological affinities and interaction between plant extracts with radiopharmaceuticals. J. Radioanal. Nucl. Chem., 2015, 306(1), 1-9.
[http://dx.doi.org/10.1007/s10967-015-4082-9]
[7]
Blum-Silva, C.H.; Luz, A.B.G.; Nascimento, M.V.P.S.; de Campos Facchin, B.M.; Baratto, B.; Fröde, T.S.; Sandjo, L.P.; Dalmarco, E.M.; Reginatto, F.H. Qualitative and quantitative analysis data of the major constituents of Ilex paraguariensis leaves by UPLC-PDA and QTOF-MS. Data Brief, 2016, 8, 295-299.
[http://dx.doi.org/10.1016/j.dib.2016.05.022] [PMID: 27331104]
[8]
Chen, M.; Lai, L.; Li, X.; Zhang, X.; He, X.; Liu, W.; Li, R.; Ke, X.; Fu, C.; Huang, Z.; Duan, C. Baicalein attenuates neurological deficits and preserves blood–brain barrier integrity in a rat model of intracerebral hemorrhage. Neurochem. Res., 2016, 41(11), 3095-3102.
[http://dx.doi.org/10.1007/s11064-016-2032-8] [PMID: 27518088]
[9]
Dave, G.S.; Galvadiya, B.; Bariya, H.; Vyas, S.R. A dataset of LC-MS QTOF analysis of potato and mustard crop residue smoke water. Data Brief, 2018, 21, 343-350.
[http://dx.doi.org/10.1016/j.dib.2018.09.117] [PMID: 30364802]
[10]
Ahn, J.; Alford, A.R.; Niemeyer, E.D. Variation in phenolic profiles and antioxidant properties among medicinal and culinary herbs of the Lamiaceae family. J. Food Meas. Charact., 2020, 14(3), 1720-1732.
[http://dx.doi.org/10.1007/s11694-020-00420-5]
[11]
Desai, A.; Qazi, G.; Ganju, R.; El-Tamer, M.; Singh, J.; Saxena, A.; Bedi, Y.; Taneja, S.; Bhat, H. Medicinal plants and cancer chemoprevention. Curr. Drug Metab., 2008, 9(7), 581-591.
[http://dx.doi.org/10.2174/138920008785821657] [PMID: 18781909]
[12]
Levorato, S.; Dominici, L.; Fatigoni, C.; Zadra, C.; Pagiotti, R.; Moretti, M.; Villarini, M. In vitro toxicity evaluation of estragole-containing preparations derived from Foeniculum vulgare Mill. (fennel) on HepG2 cells. Food Chem. Toxicol., 2018, 111, 616-622.
[http://dx.doi.org/10.1016/j.fct.2017.12.014] [PMID: 29233689]
[13]
Gokulu, S.G.; Yurt Kilcar, A.; Bilgi, A.; Karatay, K.B.; Yildirim, N.; Kayas, C.; Biber Muftuler, F.Z.; Terek, M.C. Investigation of nanoformulation and incorporation potential of radiolabeled curcumin using HeLa and MDAH-2774 cells. J. Radioanal. Nucl. Chem., 2021, 327(1), 299-305.
[http://dx.doi.org/10.1007/s10967-020-07509-7]
[14]
Asgari Lajayer, B.; Najafi, N.; Moghiseh, E.; Mosaferi, M.; Hadian, J. Micronutrient and heavy metal concentrations in basil plant cultivated on irradiated and non-irradiated sewage sludge- treated soil and evaluation of human health risk. Regul. Toxicol. Pharmacol., 2019, 104, 141-150.
[http://dx.doi.org/10.1016/j.yrtph.2019.03.009] [PMID: 30894305]
[15]
Gülçin, İ.; Elmastaş, M.; Aboul-Enein, H.Y. Determination of antioxidant and radical scavenging activity of Basil (Ocimum basilicum L. Family Lamiaceae) assayed by different methodologies. Phytother. Res., 2007, 21(4), 354-361.
[http://dx.doi.org/10.1002/ptr.2069] [PMID: 17221941]
[16]
Avcıbaşı, U.; Avcıbaşı, N.; Akalın, H.A.; Ediz, M.; Demiroğlu, H.; Gümüşer, F.G.; Özçalışkan, E.; Türkcan, C.; Uygun, D.A.; Akgöl, S. Synthesis and biodistribution of novel magnetic-poly(HEMA–APH) nanopolymer radiolabeled with iodine-131 and investigation its fate in vivo for cancer therapy. J. Nanopart. Res., 2013, 15(10), 2021.
[http://dx.doi.org/10.1007/s11051-013-2021-7]
[17]
Uzaras, C.; Avcıbaşı, U.; Demiroğlu, H.; Medine, E.İ.; Kılçar, A.Y.; Müftüler, F.Z.B.; Ünak, P. Investigation of therapeutic efficiency of phenytoin (PHT) labeled with radioactive 131I in the cancer cell lines. J. Radioanal. Nucl. Chem., 2016, 307(1), 131-140.
[http://dx.doi.org/10.1007/s10967-015-4119-0]
[18]
Avcıbaşı, U.; Türkyarar, T.; Karadağ, A.; Bakan, B.; Yavaşoğlu, N.Ü.K.; Kuşat, K.; Akgöl, S.; Gülcemal, D.; Tekin, V.; Müftüler, F.Z.B.; Topal, G.; Parlak, Y.; Gümüşer, F.G. Preparation of a 99mTc-labeled graft polymer and its in vitro and in vivo evaluation. J. Radioanal. Nucl. Chem., 2021, 329(2), 511-525.
[http://dx.doi.org/10.1007/s10967-021-07817-6]
[19]
Karatay, K.B.; Yurt Kilcar, A.; Kozgus Guldu, O.; Medine, E.I.; Biber Muftuler, F.Z. Isolation of resveratrol from peanut sprouts, radioiodination and investigation of its bioactivity on neuroblastoma cell lines. J. Radioanal. Nucl. Chem., 2020, 325(1), 75-84.
[http://dx.doi.org/10.1007/s10967-020-07222-5]
[20]
Ünak, T.; Akgün, Z.; Yildirim, Y.; Duman, Y.; Erenel, G. Self-radioiodination of iodogen. Appl. Radiat. Isot., 2001, 54(5), 749-752.
[http://dx.doi.org/10.1016/S0969-8043(00)00337-7] [PMID: 11258523]
[21]
Chalchat, J.C.; Özcan, M.M. Comparative essential oil composition of flowers, leavesand stems of basil (Ocimum basilicum L.) used as herb. Food Chem., 2008, 110(2), 501-503.
[http://dx.doi.org/10.1016/j.foodchem.2008.02.018] [PMID: 26049245]
[22]
Russo, M.; Spagnuolo, C.; Tedesco, I.; Russo, G.L. Phytochemicals in cancer prevention and therapy: truth or dare? Toxins , 2010, 2(4), 517-551.
[http://dx.doi.org/10.3390/toxins2040517] [PMID: 22069598]
[23]
Biber Muftuler, F.Z.; Unak, P. A perspective on 99mTc and 125/131I labeled receptor targeted compounds and their in vitro/in vivo affinities. J. Radioanal. Nucl. Chem., 2017, 314(1), 1-6.
[http://dx.doi.org/10.1007/s10967-017-5353-4]
[24]
Dervis, E.; Yurt Kilcar, A.; Medine, E.I.; Tekin, V.; Cetkin, B.; Uygur, E.; Muftuler, F.Z.B. In Vitro incorporation of radioiodinated Eugenol on adenocarcinoma cell lines (Caco2, MCF7, and PC3). Cancer Biother. Radiopharm., 2017, 32(3), 75-81.
[http://dx.doi.org/10.1089/cbr.2017.2181] [PMID: 28358602]
[25]
Vera-Ruiz, H.; Marcus, C.S.; Pike, V.W.; Coenen, H.H.; Fowler, J.S.; Meyer, G.J.; Cox, P.H.; Vaalburg, W.; Cantineau, R.; Helus, F.; Lambrecht, R.M. Report of an international atomic energy agency’s advisory group meeting on quality control of cyclotron-produced radiopharmaceuticals. Int. J. Rad. Appl. Instrum. B, 1990, 17(5), 445-456.
[http://dx.doi.org/10.1016/0883-2897(90)90162-T] [PMID: 2391238]
[26]
Ozkan, M.; Muftuler, F.Z.B.; Kilcar, A.Y.; Medine, E.I.; Unak, P. Isolation of hydroxytyrosol from olive leaves extract, radioiodination and investigation of bioaffinity using in vivo/in vitro methods. Radiochim. Acta, 2013, 101(9), 585-593.
[http://dx.doi.org/10.1524/ract.2013.2068]
[27]
Tekin, V.; Kozgus Guldu, O.; Medine, E.I.; Biber Muftuler, F.Z. Examination of the association between 3,4-Divanillyltetrahydrofuran Lignan (Urtica dioica Origin) and prostate cancer cells by 131I radiolabeling. Cancer Biother. Radiopharm., 2021, 36(4), 326-332.
[http://dx.doi.org/10.1089/cbr.2020.3602] [PMID: 32453606]
[28]
Karatay, K.B.; Kılçar, A.Y.; Derviş, E.; Müftüler, F.Z.B. Radioiodinated ginger compounds (6-gingerol and 6-shogaol) and incorporation assays on breast cancer cells. Anticancer. Agents Med. Chem., 2020, 20(9), 1129-1139.
[http://dx.doi.org/10.2174/1871520620666200128114215] [PMID: 31994470]
[29]
Tekin, V.; Biber Muftuler, F.Z.; Kozgus Guldu, O.; Yurt Kilcar, A.; Medine, E.I.; Yavuz, M.; Unak, P.; Timur, S. Biological affinity evaluation of Lawsonia inermis origin Lawsone compound and its radioiodinated form via in vitro methods. J. Radioanal. Nucl. Chem., 2015, 303(1), 701-708.
[http://dx.doi.org/10.1007/s10967-014-3435-0]
[30]
Enginar, H.; Ünak, P.; Lambrecht, F.Y.; Biber Müftüler, F.Z.; Medine, E.I.; Yolcular, S.; Yurt, A.; Seyitoğlu, B.; Bulduk, I. Radiolabeling of codeine with 131I and its biodistribution in rats. J. Radioanal. Nucl. Chem., 2009, 280(2), 363-370.
[http://dx.doi.org/10.1007/s10967-009-0528-2]
[31]
Enginar, H.; Unak, P.; Biber-Muftuler, F.Z.; Yurt-Lambrecht, F.; Medine, E.I.; Yolcular, S.; Yurt, A.; Seyitoglu, B.; Bulduk, I. Synthesis, radiolabeling and in vivo tissue distribution of codeine-glucuronide. INCS News, 2009, 6, 46-56.
[32]
Enginar, H.; Ünak, P.; Yurt-Lambrecht, F.; Biber-Müftüler, F.Z.; Seyitoğlu, B.; Yurt, A.; Yolcular, S.; Medine, İ.; Bulduk, İ. Radiolabeling of morphine with (131)i and its biodistribution in rats. Cancer Biother. Radiopharm., 2010, 25(4), 409-416.
[http://dx.doi.org/10.1089/cbr.2010.0774] [PMID: 20707720]
[33]
Enginar, H.; Ünak, P.; Lambrecht, F.Y.; Biber Müftüler, F.Z.; Seyitoğlu, B.; Yurt, A.; Yolcular, S.; Medine, E.İ.; Bulduk, İ. Extraction, radiolabeling, and biodistribution of thebaine in rats. Med. Chem. Res., 2013, 22(7), 3459-3465.
[http://dx.doi.org/10.1007/s00044-012-0360-z]
[34]
Enginar, H.; Unak, P.; Biber Müftüler, F.Z.; Lambrecht, F.Y.; Medine, E.I.; Yolcular, S.; Yurt Kilcar, A.; Seyitoğlu, B.; Bulduk, I. Synthesis, radiolabeling and biodistribution of morphine glucuronide (mor-glu). J. Radioanal. Nucl. Chem., 2012, 291(3), 849-857.
[http://dx.doi.org/10.1007/s10967-011-1412-4]
[35]
Enginar, H.; Unak, P.; Müftüler, F.Z.B.; Lambrecht, F.Y.; Kilcar, A.Y.; Yolcular, S.; Seyitoğlu, B.; Medine, E.I.; Bulduk, I. Synthesis, radiolabeling and biodistribution of a new opioid glucuronide derivative: ethyl-morphine glucuronide (em-glu). J. Radioanal. Nucl. Chem., 2012, 292(2), 667-673.
[http://dx.doi.org/10.1007/s10967-011-1472-5]

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