Abstract
Background: Mimusops elengi, popularly known as Bakula in Ayurvedic Medicine, is a member of the Sapotaceae family. Concerning the traditional and Ayurvedic medicinal use of Mimusops elengi leaves, especially in wound healing and oral care, the plausible presence of proteolytic activity in an aqueous Mimusops elengi leaf extract was investigated in our study.
Methods: Mimusops elengi Gelatinolytic Protease was named after fractioning the extract. The Zymogram assay validated the gelatin specificity. The effect of MEGP on the wound healing process was investigated using a different assay.
Results: The presence of protease with gelatinolytic & caseinolytic activity at 62.53±1.43 U/h and 15.31±0.64 U/h, respectively. The aqueous enzyme fraction was named Mimusops elengi Gelatinolytic Protease (MEGP). The specificity of gelatin was confirmed by zymogram. Gelatinolytic activity of MEGP was also higher than that of trypsin at 27.96±0.38 U/h. MEGP was 49.14±1.61 % inhibited by PMSF, indicating the predominant presence of serine proteases. The optimal pH and temperature for MEGP were found to be 8 and 37°C, implying that it is an alkaline protease. MEGP dissolved the blood clot at a rate of 8.41±1.04 U/h, which is higher than the trypsin clot-dissolving rate of 1.027±0.04 U/h. The plasma clot hydrolytic capacity is confirmed when MEGP hydrolyzes alpha-polymer subunits in a dose-dependent manner.
Conclusion: This investigation shows that MEGP has a plasmin-like activity that possesses a significant role in clot disintegration and other wound ailments, warranting its use in traditional Indian medicine.
Keywords: Mimusops elengi, protease, bakula, wound healing, traditional medicine, blood clot.
Graphical Abstract
Current Protein & Peptide Science
Title:Mimusops elengi (Bakula) Gelatinolytic Protease and its Plasmin-like Action on the Blood Clot
Volume: 23 Issue: 10
Author(s): Nirmala Gollarahalli Sannappa Gowda, Nithya Rani Raju, Ekaterina Silina, Victor Stupin and Raghu Ram Achar*
Affiliation:
- Division of Biochemistry, School of Life Sciences, JSS Academy of Higher Education & Research, Mysuru-570 015, Karnataka, India
Keywords: Mimusops elengi, protease, bakula, wound healing, traditional medicine, blood clot.
Abstract:
Background: Mimusops elengi, popularly known as Bakula in Ayurvedic Medicine, is a member of the Sapotaceae family. Concerning the traditional and Ayurvedic medicinal use of Mimusops elengi leaves, especially in wound healing and oral care, the plausible presence of proteolytic activity in an aqueous Mimusops elengi leaf extract was investigated in our study.
Methods: Mimusops elengi Gelatinolytic Protease was named after fractioning the extract. The Zymogram assay validated the gelatin specificity. The effect of MEGP on the wound healing process was investigated using a different assay.
Results: The presence of protease with gelatinolytic & caseinolytic activity at 62.53±1.43 U/h and 15.31±0.64 U/h, respectively. The aqueous enzyme fraction was named Mimusops elengi Gelatinolytic Protease (MEGP). The specificity of gelatin was confirmed by zymogram. Gelatinolytic activity of MEGP was also higher than that of trypsin at 27.96±0.38 U/h. MEGP was 49.14±1.61 % inhibited by PMSF, indicating the predominant presence of serine proteases. The optimal pH and temperature for MEGP were found to be 8 and 37°C, implying that it is an alkaline protease. MEGP dissolved the blood clot at a rate of 8.41±1.04 U/h, which is higher than the trypsin clot-dissolving rate of 1.027±0.04 U/h. The plasma clot hydrolytic capacity is confirmed when MEGP hydrolyzes alpha-polymer subunits in a dose-dependent manner.
Conclusion: This investigation shows that MEGP has a plasmin-like activity that possesses a significant role in clot disintegration and other wound ailments, warranting its use in traditional Indian medicine.
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Cite this article as:
Gowda Gollarahalli Sannappa Nirmala, Raju Rani Nithya, Silina Ekaterina, Stupin Victor and Achar Ram Raghu*, Mimusops elengi (Bakula) Gelatinolytic Protease and its Plasmin-like Action on the Blood Clot, Current Protein & Peptide Science 2022; 23 (10) . https://dx.doi.org/10.2174/1389203723666220829114301
DOI https://dx.doi.org/10.2174/1389203723666220829114301 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
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