Login Register Cart 0
Generic placeholder image

The Natural Products Journal

Editor-in-Chief

ISSN (Print): 2210-3155
ISSN (Online): 2210-3163

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Short Communication

In Vivo Anti-inflammatory Activity of the Crude Extract, Fractions, and Ergosterol Peroxide from Sclerotinia Sclerotiorum

Author(s): Mário F.C. Santos*, Marcela A.M. Moreira, Tatiane C.S. Maiolini, Danielle F. Dias, Daniela A. Chagas-Paula, Luciana Azevedo and Marisi G. Soares

Volume 12, Issue 7, 2022

Published on: 07 July, 2022

Article ID: e050422203104 Pages: 4

DOI: 10.2174/2210315512666220405084637

Price: $65

conference banner
Abstract

Background: Sclerotinia sclerotiorum is a phytopathogenic fungus from Sclerotinaceae family. This fungus parasitizes approximately 400 species of plants of great economic importance, such as soybean and beans. In this study, the anti-inflammatory properties of extracts, fractions, and isolated compound ergosterol peroxide were investigated. The hexane fraction and 1 inhibited croton oil-induced ear edema. In addition, ergosterol peroxide also inhibited neutrophil recruitment in the myeloperoxidase assay, demonstrating that ergosterol peroxide exerts anti-inflammatory activity, inhibiting the activity of both COX and LOX pathways. Moreover, these results provide new knowledge regarding S. sclerotiorum as a source of extracts and bioactive compounds.

Objective: The present study aimed to investigate the phytochemical profile of Sclerotinia sclerotiorum and its in vivo anti-inflammatory activity.

Methods: The crude extract, fractions, and ergosterol peroxide of Sclerotinia sclerotiorum were evaluated by croton oil-induced ear edema quantification of MPO (myeloperoxidase enzyme).

Results: The crude extract and hexane fraction exhibited edema inhibition of 50.5 and 70.6%, respectively. The ergosterol peroxide isolated from the hexane bioactive fractions exhibited significant inhibition of ear edema, indicating inhibition of the COX pathway. In addition, ergosterol peroxide inhibited neutrophil recruitment.

Conclusion: The extract and fractions, as well as the isolated compound of Sclerotinia sclerotiorum, exhibited anti-inflammatory potential.

Keywords: Sclerotinia sclerotiorum, anti-inflammatory activity, MPO, ergosterol peroxide, COX pathway, LOX pathway.

Graphical Abstract

[1]
Juliatti, F.C.; Juliatti, F.C. Podridão Branca da haste de soja: Manejo e Uso de fungicidas em busca da sustentabilidade nos sistemas de produção; Composer: Uberlândia, 2010.
[2]
Bolton, M.D.; Thomma, B.P.; Nelson, B.D. Sclerotinia sclerotiorum (Lib.) de Bary: Biology and molecular traits of a cosmopolitan pathogen. Mol. Plant Pathol., 2006, 7(1), 1-16.
[3]
Leite, R.M.V.B.C. Ocorrência de doenças causadas por Sclerotinia sclerotiorum em girassol e soja; Londrina: Embrapa Soja, (Comunicado Técnico 76), 2005.
[4]
Cotran, R.S.; Kumar, V.; Collins, T. (Trad. Barbosa, J. B.; De Vasconcelos, M. M.; Voeux, P. J.). Robbins – Patologia Estrutural e Funcional; 6th ed.; Guanabara Koogan: Rio de Janeiro, 2000.
[5]
Kummer, C.L.; Coelho, T.C.R.B. Antiinflamatórios não esteróides inibidores da ciclooxigenase-2 (COX-2): Aspectos atuais. Rev. Bras. Anestesiol., 2002, 52(4), 498-512.
[PMID: 19479115]
[6]
Coutinho, M.A.S. Flavonoides: Potenciais agentes terapêuticos para o processo de inflamatório. Revista Virtual de Química, 2009, 3, 241-256.
[7]
Calixto, J.B.; Campos, M.M.; Otuki, M.F.; Santos, A.R. Anti-inflammatory compounds of plant origin. Part II. modulation of pro-inflammatory cytokines, chemokines and adhesion molecules. Planta Med., 2004, 70(2), 93-103.
[http://dx.doi.org/10.1055/s-2004-815483] [PMID: 14994184]
[8]
Nantel, F.; Denis, D.; Gordon, R.; Northey, A.; Cirino, M.; Metters, K.M.; Chan, C.C. Distribution and regulation of cyclooxygenase-2 in carrageenan-induced inflammation. Br. J. Pharmacol., 1999, 128(4), 853-859.
[http://dx.doi.org/10.1038/sj.bjp.0702866] [PMID: 10556918]
[9]
Betina, V. The chemistry and biology of antibiotics; Elsevier: Amsterdam, 1983.
[10]
Bérdy, J. Bioactive microbial metabolites. J. Antibiot. (Tokyo), 2005, 58(1), 1-26.
[http://dx.doi.org/10.1038/ja.2005.1] [PMID: 15813176]
[11]
Pressete, C.G.; Giannini, L.S.V.; de Paula, D.A.C.; do Carmo, M.A.V.; Assis, D.M.; Santos, M.F.C.; Machado, J.D.C.; Marques, M.J.; Soares, M.G.; Azevedo, L. Sclerotinia sclerotiorum (white mold): Cytotoxic, mutagenic, and antimalarial effects in vivo and in vitro. J. Food Sci., 2019, 84(12), 3866-3875.
[http://dx.doi.org/10.1111/1750-3841.14910] [PMID: 31750949]
[12]
Zhao, M.; Ruan, Q.; Pan, W.; Tang, Y.; Zhao, Z.; Cui, H. New polyketides and diterpenoid derivatives from the fungus Penicillium scleroti-orum GZU-XW03-2 and their anti-inflammatory activity. Fitoterapia, 2020, 143104561.
[http://dx.doi.org/10.1016/j.fitote.2020.104561] [PMID: 32199959]
[13]
Ji, H-F.; Li, X-J.; Zhang, H-Y. Natural products and drug discovery. Can thousands of years of ancient medical knowledge lead us to new and powerful drug combinations in the fight against cancer and dementia? EMBO Rep., 2009, 10(3), 194-200.
[http://dx.doi.org/10.1038/embor.2009.12] [PMID: 19229284]
[14]
Cragg, G.M.; Newman, D.J. Natural products: A continuing source of novel drug leads. Biochim. Biophys. Acta, 2013, 1830(6), 3670-3695.
[http://dx.doi.org/10.1016/j.bbagen.2013.02.008] [PMID: 23428572]
[15]
Kim, D.S.; Back, N-I. OH, S.R.; Jung, K.Y.; Lee, I.S.; Kim, J.H.; Lee, H.-K. Anticomplementary activity of ergosterol peroxide from Naema-toloma fasciculare and reassignment of NMR Data. Arch. Pharm. Res., 1997, 20, 201.
[http://dx.doi.org/10.1007/BF02976145] [PMID: 18975152]
[16]
Tubaro, A.; Dri, P.; Delbello, G.; Zilli, C.; Della Loggia, R. The croton oil ear test revisited. Agents Actions, 1986, 17(3-4), 347-349.
[http://dx.doi.org/10.1007/BF01982641] [PMID: 3962781]
[17]
Santos, M.F.C.; Alcântara, B.G.V.; Feliciano, C.R.; Silva, A.F.; Maiolini, T.C.S.; Katchborian-Neto, A.; Murgu, M.; Chagas-Paula, D.A.; Soa-res, M.G. New bicyclic [3.2.1] octane neolignans derivatives from Aniba firmula with potent in vivo anti-inflammatory activity on account of dual inhibition of PGE2 production and cell recruitment. Phytochem. Lett., 2019, 30, 31-37.
[http://dx.doi.org/10.1016/j.phytol.2019.01.014]
[18]
Phatangare, N.D.; Deshmukh, K.K.; Murade, V.D.; Naikwadi, P.H.; Hase, D.P.; Chavhan, M.J.; Velis, H.E. Isolation and characterization of β-sitosterol from Justicia gendarussa burm. F.-An anti-inflammatory compound. Int. J. Pharmacogn. Phytochem. Res., 2017, 9(9), 1280-1287.
[http://dx.doi.org/10.25258/phyto.v9i09.10317]
[19]
Kobori, M.; Yoshida, M.; Ohnishi-Kameyama, M.; Shinmoto, H. Ergosterol peroxide from an edible mushroom suppresses inflammatory responses in RAW264.7 macrophages and growth of HT29 colon adenocarcinoma cells. Br. J. Pharmacol., 2007, 150(2), 209-219.
[http://dx.doi.org/10.1038/sj.bjp.0706972] [PMID: 17160010]

Rights & Permissions Print Cite
Article Metrics
10
© 2024 Bentham Science Publishers | Privacy Policy