Login Register Cart 0
Generic placeholder image

Current Bioactive Compounds

Editor-in-Chief

ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Phenolic Compounds and Antioxidant and Antimicrobial Activities of Pulicaria Odora Extract

Author(s): Karima Saidani*, Naima Touati, Hafida Merzouk, Halim Boussaa, Fatiha Bedjou and B. Dave Oomah

Volume 19, Issue 1, 2023

Published on: 19 August, 2022

Article ID: e040422203044 Pages: 10

DOI: 10.2174/1573407218666220404094002

Price: $65

Abstract

Background: The Pulicaria genus (Asteraceae family) plays a remarkable role in the development of drugs used in modern medicine. Leaf extracts of Pulicaria odora harvested in Bejaia were evaluated for their antioxidant and antimicrobial activities.

Methods: HCl method at three different wavelengths (280, 320, and 360 nm) was carried out for total phenolic content and characterized by ultra-performance liquid chromatography (UPLC) analysis. Antimicrobial activity was evaluated using four bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Listeria innocua) and three molds (Aspergillus flavus, Aspergillus niger, and Trichoderma reesei). Four antioxidant tests (ORAC, ABTS, DPPH, and FRAP) were performed.

Results: The extracts had high total polyphenols (305 mg catechin equivalents [CE]), flavonols (56 mg quercetin equivalents [QE]), tannins (77 mg CE) and tartaric acids (38 mg caffeic acid equivalents [CAE]) per g dry matter contents. UPLC analysis of the 70% ethanol extracts showed abundant phenolic acids (protocatechuic, chlorogenic, and caffeic acids). Pulicaria odora extracts exhibited good antimicrobial activity against bacterial (~18 mm inhibition zones) and molds (11.3- 17.3 mm) strains.

Conclusion: All extracts displayed good antioxidant activity and effective antimicrobial activity, enabling their use as a conservative agent or therapeutic remedy.

Keywords: antimicrobial activity, antioxidant activity, phenolic compounds, Pulicaria odora, UPLC

Graphical Abstract

[1]
Inoue, M.; Hayashi, S.; Craker, L.E. Role of medicinal and aromatic plants: Past, present, and future; Pharmacognosy-Medicinal Plants, 2019, pp. 1-13.
[2]
Khazir, J.; Mir, B.A.; Pilcher, L.; Riley, D.L. Role of plants in anticancer drug discovery. Phytochem. Lett., 2014, 7, 173-181.
[http://dx.doi.org/10.1016/j.phytol.2013.11.010]
[3]
Lemus-Mondaca, R.; Vega-Gálvez, A.; Zura-Bravo, L.; Ah-Hen, K. Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chem., 2012, 132(3), 1121-1132.
[http://dx.doi.org/10.1016/j.foodchem.2011.11.140] [PMID: 29243591]
[4]
Robards, K.; Prenzler, P.D.; Tucker, G.; Swatsitang, P.; Glover, W. Phenolic compounds and their role in oxidative processes in fruits. Food Chem., 1999, 66(4), 401-436.
[http://dx.doi.org/10.1016/S0308-8146(99)00093-X]
[5]
Hussien, T.A.; El-Toumy, S.; Hassan, H.; Hetta, M. Cytotoxic and antioxidant activities of secondary metabolites from Pulicaria undulata. Int. J. Pharm. Pharm. Sci., 2016, 8(9), 150-155.
[http://dx.doi.org/10.22159/ijpps.2016v8i9.12814]
[6]
Williams, C.A.; Harborne, J.B.; Greenham, J.R.; Grayer, R.J.; Kite, G.C.; Eagles, J. Variations in lipophilic and vacuolar flavonoids among European Pulicaria species. Phytochemistry, 2003, 64(1), 275-283.
[http://dx.doi.org/10.1016/S0031-9422(03)00207-3] [PMID: 12946426]
[7]
Al-Fatimi, M. Ethnobotanical survey of medicinal plants in central Abyan Governorate, Yemen. J. Ethnopharmacol., 2019, 241, 111973.
[http://dx.doi.org/10.1016/j.jep.2019.111973] [PMID: 31146001]
[8]
Yebouk, C.; Redouan, F.Z.; Benítez, G.; Bouhbal, M.; Kadiri, M.; Boumediana, A.I.; Molero-Mesa, J.; Merzouki, A. Ethnobotanical study of medicinal plants in the Adrar Province, Mauritania. J. Ethnopharmacol., 2020, 246, 112217.
[http://dx.doi.org/10.1016/j.jep.2019.112217] [PMID: 31520672]
[9]
Hussein, S.R.; Marzouk, M.M.; Soltan, M.M.; Ahmed, E.K.; Said, M.M.; Hamed, A.R. Phenolic constituents of Pulicaria undulata (L.) C.A. Mey. sub sp. undulata (Asteraceae): Antioxidant protective effects and chemosystematic significances. J. Food Drug Anal., 2017, 25(2), 333-339.
[http://dx.doi.org/10.1016/j.jfda.2016.09.008] [PMID: 28911675]
[10]
Hanbali, F.E.; Akssira, M.; Ezoubeiri, A.; Gadhi, C.E.; Mellouki, F.; Benherraf, A.; Blazquez, A.M.; Boira, H. Chemical composition and antibacterial activity of essential oil of Pulicaria odora L. J. Ethnopharmacol., 2005, 99(3), 399-401.
[http://dx.doi.org/10.1016/j.jep.2005.01.012] [PMID: 15896934]
[11]
Touati, N.; Saidani, K.; Boudries, H.; Hammiche, H.; Ouazene, N.; Bedjou, F. Antibacterial activity of phenolic compounds of Pulicaria odora, wild plant in northern Algeria. Int. Food Res. J., 2018, 25(5), 21-30.
[12]
Oomah, B.D. Cardador‐Martínez, A.; Loarca‐Piña, G. Phenolics and antioxidative activities in common beans (Phaseolus vulgaris L). J. Sci. Food Agric., 2005, 85(6), 935-942.
[http://dx.doi.org/10.1002/jsfa.2019]
[13]
Ross, K.A.; Zhang, L.; Arntfield, S.D. Understanding water uptake from the induced changes occurred during processing: Chemistry of pinto and navy bean seed coats. Int. J. Food Prop., 2010, 13(3), 631-647.
[http://dx.doi.org/10.1080/10942910902718220]
[14]
Prior, R.L.; Hoang, H.; Gu, L.; Wu, X.; Bacchiocca, M.; Howard, L.; Hampsch-Woodill, M.; Huang, D.; Ou, B.; Jacob, R. Assays for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity (ORAC(FL)) of plasma and other biological and food samples. J. Agric. Food Chem., 2003, 51(11), 3273-3279.
[http://dx.doi.org/10.1021/jf0262256] [PMID: 12744654]
[15]
Benzie, I.F.; Strain, J.J. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal. Biochem., 1996, 239(1), 70-76.
[http://dx.doi.org/10.1006/abio.1996.0292] [PMID: 8660627]
[16]
Brand-Williams, W.; Cuvelier, M.; Berset, C. Use of a free radical method to evaluate antioxidant activity. Lebensm. Wiss. Technol., 1995, 28(1), 25-30.
[http://dx.doi.org/10.1016/S0023-6438(95)80008-5]
[17]
Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med., 1999, 26(9-10), 1231-1237.
[http://dx.doi.org/10.1016/S0891-5849(98)00315-3] [PMID: 10381194]
[18]
Suay, I.; Arenal, F.; Asensio, F.J.; Basilio, A.; Cabello, M.A.; Díez, M.T.; García, J.B.; del Val, A.G.; Gorrochategui, J.; Hernández, P.; Peláez, F.; Vicente, M.F. Screening of basidiomycetes for antimicrobial activities. Antonie van Leeuwenhoek, 2000, 78(2), 129-139.
[http://dx.doi.org/10.1023/A:1026552024021] [PMID: 11204765]
[19]
Karabay-Yavasoglu, N.U.; Sukatar, A.; Ozdemir, G.; Horzum, Z. Antimicrobial activity of volatile components and various extracts of the red alga Jania rubens. Phytother. Res., 2007, 21(2), 153-156.
[http://dx.doi.org/10.1002/ptr.2045] [PMID: 17128433]
[20]
Bansemir, A.; Blume, M.; Schröder, S.; Lindequist, U. Screening of cultivated seaweeds for antibacterial activity against fish pathogenic bacteria. Aquaculture, 2006, 252(1), 79-84.
[http://dx.doi.org/10.1016/j.aquaculture.2005.11.051]
[21]
Meyer, A.; Deiana, J.; Bernard, A. Cours de microbiologie générale; Doin, 1994, p. 365.
[22]
Tuncel, G.; Nergiz, C. Antimicrobial effect of some olive phenols in a laboratory medium. Lett. Appl. Microbiol., 1993, 17(6), 300-302.
[http://dx.doi.org/10.1111/j.1472-765X.1993.tb01472.x]
[23]
Algabr, M.N.; Mekkiou, R.; Ameddah, S.; Menad, A.; Boumaza, O.; Seghiri, R.; Benayache, F. Antioxidant activities from the aerial parts of Pulicaria jaubertii. American Eurasian Network for Scientific Information, 2010, 4(1), 63-70.
[24]
Pinelo, M.; Manzocco, L.; Nuñez, M.J.; Nicoli, M.C. Interaction among phenols in food fortification: Negative synergism on antioxidant capacity. J. Agric. Food Chem., 2004, 52(5), 1177-1180.
[http://dx.doi.org/10.1021/jf0350515] [PMID: 14995117]
[25]
Dai, J.; Mumper, R.J. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules, 2010, 15(10), 7313-7352.
[http://dx.doi.org/10.3390/molecules15107313] [PMID: 20966876]
[26]
Xu, B.J.; Chang, S.K. A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J. Food Sci., 2007, 72(2), S159-S166.
[http://dx.doi.org/10.1111/j.1750-3841.2006.00260.x] [PMID: 17995858]
[27]
Generalić I.; Skroza, D.; Šurjak, J.; Možina, S.S.; Ljubenkov, I.; Katalinić A.; Šimat, V.; Katalinić V. Seasonal variations of phenolic compounds and biological properties in sage (Salvia officinalis L.). Chem. Biodivers., 2012, 9(2), 441-457.
[http://dx.doi.org/10.1002/cbdv.201100219] [PMID: 22344920]
[28]
Benhouda, A.; Yahia, M. Toxicity, analgesic and anti-pyretic activities of methanolic extract from Hyoscyamus albus leaves in albino rats. Int. J. Pharm. Pharm. Sci., 2014, 6(3), 121-127.
[29]
Pares, J.O.; Oksuz, S.; Ulubelen, A.; Mabry, T. 6-Hydroxyflavonoids from Pulicaria dysenterica (compositae). Phytochemistry, 1981, 20(8), 2057-2057.
[http://dx.doi.org/10.1016/0031-9422(81)84076-9]
[30]
El-Negoumy, S.I.; Mansour, R.M.; Saleh, N.A. Flavonols of Pulicaria arabica. Phytochemistry, 1982, 21(4), 953-954.
[http://dx.doi.org/10.1016/0031-9422(82)80105-2]
[31]
Liu, L.L.; Yang, J.L.; Shi, Y.P. Phytochemicals and biological activities of Pulicaria species. Chem. Biodivers., 2010, 7(2), 327-349.
[http://dx.doi.org/10.1002/cbdv.200900014] [PMID: 20151381]
[32]
Tawaha, K.; Alali, F.Q.; Gharaibeh, M.; Mohammad, M.; El-Elimat, T. Antioxidant activity and total phenolic content of selected Jordanian plant species. Food Chem., 2007, 104(4), 1372-1378.
[http://dx.doi.org/10.1016/j.foodchem.2007.01.064]
[33]
Wojdylo, A.; Oszmianski, J.; Czemerys, R. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem., 2007, 105(3), 940-949.
[http://dx.doi.org/10.1016/j.foodchem.2007.04.038]
[34]
Ghimire, B.K.; Seong, E.S.; Kim, E.H.; Ghimeray, A.K.; Yu, C.Y.; Ghimire, B.K.; Chung, I.M. A comparative evaluation of the antioxidant activity of some medicinal plants popularly used in Nepal. J. Med. Plants Res., 2011, 5(10), 1884-1891.
[35]
Mothana, R.A.; Abdo, S.A.; Hasson, S.; Althawab, F.M.; Alaghbari, S.A.; Lindequist, U. Antimicrobial, antioxidant and cytotoxic activities and phytochemical screening of some yemeni medicinal plants. Evidence-based complementary and alternative medicine. ECAM, 2010, 7(3), 323-330.
[36]
Prior, R.L.; Cao, G. In vivo total antioxidant capacity: Comparison of different analytical methods. Bio-Assays for Oxidative Stress Status; Elsevier, 2001, pp. 39-47.
[http://dx.doi.org/10.1016/B978-0-444-50957-4.50009-0]
[37]
Arnao, M.B. Some methodological problems in the determination of antioxidant activity using chromogen radicals: A practical case. Trends Food Sci. Technol., 2000, 11(11), 419-421.
[http://dx.doi.org/10.1016/S0924-2244(01)00027-9]
[38]
Alzoreky, N.S.; Nakahara, K. Antibacterial activity of extracts from some edible plants commonly consumed in Asia. Int. J. Food Microbiol., 2003, 80(3), 223-230.
[http://dx.doi.org/10.1016/S0168-1605(02)00169-1] [PMID: 12423924]
[39]
Ali, N.A.; Jülich, W-D.; Kusnick, C.; Lindequist, U. Screening of Yemeni medicinal plants for antibacterial and cytotoxic activities. J. Ethnopharmacol., 2001, 74(2), 173-179.
[http://dx.doi.org/10.1016/S0378-8741(00)00364-0] [PMID: 11167035]
[40]
Nickavar, B.; Mojab, F. Antibacterial activity of Pulicaria dysenterica extracts. Fitoterapia, 2003, 74(4), 390-393.
[http://dx.doi.org/10.1016/S0367-326X(03)00060-1] [PMID: 12781813]
[41]
Mothana, R.A.; Lindequist, U. Antimicrobial activity of some medicinal plants of the island Soqotra. J. Ethnopharmacol., 2005, 96(1-2), 177-181.
[http://dx.doi.org/10.1016/j.jep.2004.09.006] [PMID: 15588668]
[42]
El-Kamali, H.H.; Mahjoub, S.A-T. Antibacterial activity of Francoeuria crispa, Pulicaria undulata, Ziziphus spina-christi and Cucurbita pepo against seven standard pathogenic bacteria. Ethnobotanical Leaflets, 2009, 2009(6), 1-6.
[43]
Mohamed, E.A.A.; Muddathir, A.M.; Osman, M.A. Antimicrobial activity, phytochemical screening of crude extracts, and essential oils constituents of two Pulicaria spp. growing in Sudan. Sci. Rep., 2020, 10(1), 17148.
[http://dx.doi.org/10.1038/s41598-020-74262-y] [PMID: 33051571]
[44]
Karabegović I.; Nikolova, M.; Veličković D.; Stojičević S.; Veljković V.; Lazić M. Comparison of antioxidant and antimicrobial activities of methanolic extracts of the Artemisia sp. recovered by different extraction techniques. Chin. J. Chem. Eng., 2011, 19(3), 504-511.
[http://dx.doi.org/10.1016/S1004-9541(11)60013-X]
[45]
Salie, F.; Eagles, P.F.; Leng, H.M. Preliminary antimicrobial screening of four South African Asteraceae species. J. Ethnopharmacol., 1996, 52(1), 27-33.
[http://dx.doi.org/10.1016/0378-8741(96)01381-5] [PMID: 8733116]
[46]
Stojković D.; Petrović J.; Soković M.; Glamočlija, J.; Kukić-Marković J.; Petrović S. In situ antioxidant and antimicrobial activities of naturally occurring caffeic acid, p-coumaric acid and rutin, using food systems. J. Sci. Food Agric., 2013, 93(13), 3205-3208.
[http://dx.doi.org/10.1002/jsfa.6156] [PMID: 23553578]
[47]
Borges, A.; Ferreira, C.; Saavedra, M.J.; Simões, M. Antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria. Microb. Drug Resist., 2013, 19(4), 256-265.
[http://dx.doi.org/10.1089/mdr.2012.0244] [PMID: 23480526]
[48]
Nath, E.Ö.; Bilgin, M.; Gurdal, B. Antimicrobial activity of Pulicaria species from Turkey. Experimed, 2021, 11(3), 195-199.
[http://dx.doi.org/10.26650/experimed.2021.986570]
[49]
Pendota, S.; Aderogba, M.; Van Staden, J. In vitro antimicrobial activity of extracts and an isolated compound from Boscia albitrunca leaves. S. Afr. J. Bot., 2015, 96, 91-93.
[http://dx.doi.org/10.1016/j.sajb.2014.11.005]
[50]
Diarra, M.S.; Hassan, Y.I.; Block, G.S.; Drover, J.C.; Delaquis, P.; Oomah, B.D. Antibacterial activities of a polyphenolic-rich extract prepared from American cranberry (Vaccinium macrocarpon) fruit pomace against Listeria spp. J. Contemp. Dent. Pract., 2020, 21(5), 500-508.
[PMID: 32690831]
[51]
Okoro, I.O.; Osagie, A.; Asibor, E.O. Antioxidant and antimicrobial activities of polyphenols from ethnomedicinal plants of Nigeria. Afr. J. Biotechnol., 2010, 9(20), 2989-2993.
[52]
Russell, A.L.; Kennedy, A.M.; Spuches, A.M.; Venugopal, D.; Bhonsle, J.B.; Hicks, R.P. Spectroscopic and thermodynamic evidence for antimicrobial peptide membrane selectivity. Chem. Phys. Lipids, 2010, 163(6), 488-497.
[http://dx.doi.org/10.1016/j.chemphyslip.2010.03.009] [PMID: 20362562]
[53]
Aziz, N.H.; Farag, S.E.; Mousa, L.A.; Abo-Zaid, M.A. Comparative antibacterial and antifungal effects of some phenolic compounds. Microbios, 1998, 93(374), 43-54.
[PMID: 9670554]
[54]
Mahoney, N.; Molyneux, R.; Kim, J.; Campbell, B.; Waiss, A.; Hagerman, A. Aflatoxigenesis induced in Aspergillus flavus by oxidative stress and reduction by phenolic antioxidants from tree nuts. World Mycotoxin J., 2010, 3(1), 49-57.
[http://dx.doi.org/10.3920/WMJ2009.1185]

Rights & Permissions Print Cite
Article Metrics
1
© 2024 Bentham Science Publishers | Privacy Policy