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Current Materials Science

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ISSN (Print): 2666-1454
ISSN (Online): 2666-1462

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General Research Article

Bio-Application of Poly (Vinyl Alcohol)/Biphasic Calcium Phosphate Scaffold as Bone Tissue Replacement

Author(s): Wafaa A. Hussain, Mukhlis M. Ismail* and Furqan Salim Hashim

Volume 15, Issue 3, 2022

Published on: 14 June, 2022

Page: [271 - 279] Pages: 9

DOI: 10.2174/2666145415666220330110601

Price: $65

Abstract

Background: Porous scaffolds composed of poly (vinyl alcohol)/Biphasic calcium phosphate (PVA/BCP) were prepared for bone tissue engineering. The effect of BCP was investigated on the morphology of pores, porosity, compression strength, swelling ratio, biodegradation, bioactivity, and in vivo blood count.

Objective: the aim of the article is to prepare a porous scaffold with good mechanical properties and suitable for the living body by having biodegradable, bioactive, and biocompatible properties.

Methods: The scaffold of PVA foam was prepared using 4g of PVA (Central Drug House, M.W. 13000-23000, Viscosity 3.5-4.5, Hydrolysis 87-89%, PH 4.5-6.5, India) dissolved in 24 ml distilled water with stirrer for 5 minutes. The biphasic-calcium phosphate (BCP) (Ying Tong Chem and Tech, LTD, Density 3.14 g/cm3, China) was added to the solution with heat stirring for 5 minutes with a ratio of 0, 0.05, 0.1 and 0.25 with respect to the weight of PVA.

The sulfuric acid (Central Drug House, M.W. 98.08, India) of (8 ml) was added drop by drop using a catalyzer. The citric acid (Central Drug House, M.W. 192.13, India) was added to the mixture with 24g to create esterification bonding.

Results: The results showed that the scaffold using BCP had uniform pore size distribution, suitable porosity up to 67%, and showed high swelling ratio. The scaffolds were of biodegradable nature and almost degraded by about 37.5% in four weeks. The scaffold was biologically active in terms of the presence of calcium phosphate in the hydroxyapatite phase as in bone. The in vivo biocompatibility of the PVA/BCP scaffold was tested by comparing the blood count with the normal range of blood in rabbits. After 14 days, the Blood Urea, Creatinine, A total of Bilirubin, and Lymphocytes were higher than the control.

Conclusion: The addition of BCP powder has a positive effect on porosity and pore size. The compression strength value increased significantly with the use of BCP from 2.12 to 5.29 MPa. The scaffolds show good biodegradation and well bioactivity. The culture of the biomaterial caused toxicity or an acute inflammatory response, as the blood test results showed that there was infiltration of polymorphous leukocytes, lymphocytes, macrophages, and fibroblasts.

Keywords: In vivo, in vitro, bone tissue engineering, PVA, Biphasic calcium phosphate, scaffold.

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[1]
Kashirina A, Yao Y, Liu Y, Leng J. Biopolymers for bone substitutes: A review. Biomater Sci 2019; 7(10): 3961-83.
[2]
Fergal J, Brien O. Biomaterials & scaffolds for tissue engineering. Mater Today 2011; 14(3): 88-95.
[3]
Neto AS, Ferreira JMF. Synthetic and marine-derived porous scaffolds for bone tissue engineering. Materials (Basel) 2018; 11(9): 1-40.
[http://dx.doi.org/10.3390/ma11091702] [PMID: 30216991]
[4]
Biswal T. Biopolymers for tissue engineering applications: A review. Mater Today Proc 2021; 41: 397-402.
[http://dx.doi.org/10.1016/j.matpr.2020.09.628]
[5]
Dhandayuthapani B, Yoshida Y, Maekawa T, Kumar DS. Polymeric scaffolds in tissue engineering application: A review. Int J Polym Sci 2011; 2011: 1-19.
[http://dx.doi.org/10.1155/2011/290602]
[6]
Shi C, Yuan Z, Han F, Zhu C, Li B. Polymeric biomaterials for bone regeneration. Ann Joint 2016; 1: 27.
[http://dx.doi.org/10.21037/aoj.2016.11.02]
[7]
Jiang S, Liu S, Feng W. PVA hydrogel properties for biomedical application. J Mech Behav Biomed Mater 2011; 4(7): 1228-33.
[http://dx.doi.org/10.1016/j.jmbbm.2011.04.005] [PMID: 21783131]
[8]
Ashraf AA, Zebarjad SM, Hadianfard MJ. The cross-linked polyvinyl alcohol/hydroxyapatite nanocomposite foam. J Mater Res Technol 2019; 8(3): 3149-57.
[http://dx.doi.org/10.1016/j.jmrt.2019.02.024]
[9]
Li YH, Wang ZD, Wang W, Ding CW, Zhang HX, Li JM. The biocompatibility of calcium phosphate cements containing alendronate-loaded PLGA microparticles in vitro. Exp Biol Med 2015; 240(11): 1465-71.
[http://dx.doi.org/10.1177/1535370215579142]
[10]
Bhowmick A, Pramanik N, Mitra T, Gnanamani A, Dasc M, Kundu PP. Mechanical and biological investigations of chitosan-polyvinyl alcohol based ZrO2 doped porous hybrid composites for bone tissue engineering applications. New J Chem 2017; 41(15): 7524-30.
[11]
Hussain WA, Hadi EM, Ismail MM, Alwan LH. Preparation of household water filter. J Appl Eng Sci 2020; 23(1): 61-8.
[12]
Thomas LV, Arun U, Remya S, et al. A biodegradable and biocompatible PVA–citric acid polyester with potential applications as ma-trix for vascular tissue engineering. J Mater Sci Mater Med 2009; 20(S1): 259-69.
[http://dx.doi.org/10.1007/s10856-008-3599-7]
[13]
Wang Z, Tang Z, Qing F, Hong Y, Zhang X. Applications of calcium phosphate nanoparticles in porous hard tissue engineering scaf-folds. In: Nano. 2012; 7: p. (4)230004.
[http://dx.doi.org/10.1142/S1793292012300046]
[14]
Tang Z, Li X, Tan Y, Fan H, Zhang X. The material and biological characteristics of osteoinductive calcium phosphate ceramics. Regen Biomater 2018; 5(1): 43-59.
[http://dx.doi.org/10.1093/rb/rbx024]
[15]
Gan D, Liu M, Xu T, Wang K, Tan H, Xiong LU. Chitosan/biphasic calcium phosphate scaffolds functionalized with BMP-2-encapsulated Nanoparticles and RGD for Bone Regeneration. J Biomed Mater Res 2018; 106(10): 2613-4.
[http://dx.doi.org/10.1002/jbm.a.36453]
[16]
Chen G, Chen N, Wang Q. Fabrication and properties of poly(vinyl alcohol)/β-tricalcium phosphate composite scaffolds via fused deposition modeling for bone tissue engineering. Compos Sci Technol 2019; 172: 17-28.
[http://dx.doi.org/10.1016/j.compscitech.2019.01.004]
[17]
Chocholata P, Kulda V, Dvorakova J, Kolaja Dobra J, Babuska V. Biological evaluation of polyvinyl alcohol hydrogels enriched by hyaluronic acid and hydroxyapatite. Int J Mol Sci 2020; 21(16): 5719.
[http://dx.doi.org/10.3390/ijms21165719] [PMID: 32784986]
[18]
Mahnama H, Dadbin S, Frounchi M, Rajabi S. Preparation of biodegradable gelatin/PVA porous scaffolds for skin regeneration. Artif Cells Nanomed Biotechnol 2017; 45(5): 928-35.
[19]
Hussain WA, Al-Mosawe Entessar HA, Ismail Mukhlis M, Alwan Luay H. Porous biphasic calcium phosphate for biomedical appli-cation. J Biomim Biomater Biomed Eng 2021; 49: 101-10.
[20]
Kandulna R, Choudhary RB. Concentration-dependent behaviors of ZnO-reinforced PVA–ZnO nanocomposites as electron transport materials for OLED application. Polym Bull 2018; 75(7): 3089-107.
[http://dx.doi.org/10.1007/s00289-017-2186-9]
[21]
Washington IM, Van Hoosier G. Clinical Biochemistry and Hematology American College of Laboratory Animal Medicine. Seattle, WA, USA: University of Washington 2012; pp. 57-116.
[22]
Moore DM, Zimmerman K, Smith Stephen A. Hematology of laboratory rabbits Elsevier J Vet Clin Exot Anim 2015; 18: 9-19.
[http://dx.doi.org/10.1016/j.cvex.2014.09.003]
[23]
Bhardwaj U, Sura R, Papadimitrakopoulos F, Burgess DJ. Controlling acute inflammation with fast releasing dexamethasone-PLGA microsphere/PVA hydrogel composites for implantable devices. J Diabetes Sci Technol 2007; 1(1): 8-17.
[http://dx.doi.org/10.1177/193229680700100103] [PMID: 19888374]
[24]
Zhang J, Yuan K, Wang YP, Zhang ST. Preparation and PH responsive behavior of poly(Vinyl Alcohol)–chitosan–poly(acrylic acid) full-IPN hydrogels. J Bioact Compat Polym 2007; 22(2): 207-18.
[http://dx.doi.org/10.1177/0883911506076046]
[25]
Kamarul T, Krishnamurithy G, Salih ND. Biocompatibility and toxicity of poly(vinyl alcohol)/N,O-carboxymethyl chitosan scaffold. Sci World J 2014; 2014: 905103.

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