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ISSN (Print): 2211-5501
ISSN (Online): 2211-551X

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Research Article

Prediction of Secondary and Tertiary Structure and Docking of Rb1WT And Rb1R661W Proteins

Author(s): Aimen Sajid, Muhammad Shaoor Saeed, Rabbiah Manzoor Malik*, Sahar Fazal, Shaukat Malik and Mohammad Amjad Kamal

Volume 11, Issue 1, 2022

Published on: 08 March, 2022

Page: [71 - 85] Pages: 15

DOI: 10.2174/2211550111666220127100203

Price: $65

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Abstract

Background: Retinoblastoma, a malignancy occurring in the juvenile cells of the retina, is responsible for light detection. It is one of the most emerging ra re childhood and infant cancer. It is initiated by the mutation in Rb1, a first tumor suppressor gene located on chromosome 13q14. Rb1 protein is responsible for cell cycle regulation.

Methods: In our study, secondary and 3D-Structural predictions of Rb1WT and Rb1R661W were made by comparative or homology modeling to find any structural change leading to the disruption in its further interactions. Quality assurance of the structures was done by Ramachandran Plot for a stable structure. Both the proteins were then applied by docking process with proteins of interest.

Results: Secondary structure showed a number of mutations in helixes, β-Hairpins of Rb1R661W. The major change was the loss of β-Hairpin loop, extension and shortening of helixes. 3D comparison structure showed a change in the groove of Rb1R661W. Docking results, unlike RB1 WT, had different and no interactions with some of the proteins of interest. This mutation in Rb1 protein had a deleterious effect on the protein functionality.

Conclusion: This study will help to design the appropriate therapy and also understand the mechanism of disease of retinoblastoma, for researchers and pharmaceuticals.

Keywords: Retinoblastoma, cancer, Rb protein, Rb1WT, Rb1R661W, CDK’s family, E2F family, docking.

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