Promotion of Cervical Cancer Cell Proliferation by miR-130b Expression
Level Changes and Inhibition of its Apoptosis by Targeting CDKN1A
Gene

doi:10.2174/1568009622666220111090715

摘要

背景：miR-130b 表达的失调与不同癌症的发展有关。然而，关于 miR-130b 在宫颈癌细胞生长和存活中的生物学作用的描述是有限的。方法：采用逆转录-定量 PCR 方法测定宫颈癌细胞不同生长阶段的 miR-130b 水平。使用基于 SYBR Green 的定量甲基化特异性 PCR 测量 miR-130b 基因上游 DNA 序列的甲基化水平。逆转录定量 PCR、Western 印迹和荧光报告测定用于鉴定 miR-130b 靶向基因。细胞计数试剂盒 8 和彗星测定分别用于确定细胞中的细胞活力和 DNA 损伤水平。 EdU Apopllo488体外流式细胞仪试剂盒、碘化丙啶染色、抗-γ-H2AX抗体染色和Annexin-V凋亡试剂盒随后用于测定DNA合成速率、细胞周期分布、DNA双链断裂计数和水平凋亡细胞。结果： miR-130b 水平在宫颈癌细胞生长的指数期增加，但在稳定期降低。转录起始位点附近突出的 CpG 岛的甲基化抑制了 miR-130b 基因的表达。 MiR-130b 增加细胞活力，促进 DNA 合成和指数期细胞的 G1 期到 S 期转变，但通过靶向细胞周期蛋白依赖性降低细胞活力，伴随着 DNA 断裂的积累和静止期细胞凋亡率的增加激酶抑制剂 1A mRNA。结论：miR-130b在指数期促进宫颈癌细胞的生长，而在静止期损害细胞的存活。

关键词: 动力学、miR-130b、表达水平变化、宫颈癌细胞、生长、存活。

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DOI https://dx.doi.org/10.2174/1568009622666220111090715	Print ISSN 1568-0096
Publisher Name Bentham Science Publisher	Online ISSN 1873-5576

当代肿瘤药物靶点

miR-130b表达水平变化促进宫颈癌细胞增殖并靶向CDKN1A基因抑制其凋亡

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当代肿瘤药物靶点

miR-130b表达水平变化促进宫颈癌细胞增殖并靶向CDKN1A基因抑制其凋亡

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