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Current Enzyme Inhibition

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ISSN (Print): 1573-4080
ISSN (Online): 1875-6662

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Research Article

The Inhibitory Effect of Hydroalcoholic Extract from the Algerian Echium trygorrhizum Pomel Roots on α-amylase Activity

Author(s): Allaoua Nouri*, Lakhder Gasmi, Chawki Bensouici, Daoud Harzallah, Seddik Khennouf and Saliha Dahamna

Volume 18, Issue 1, 2022

Published on: 15 March, 2022

Page: [40 - 46] Pages: 7

DOI: 10.2174/1573408018666211224092636

Price: $65

Abstract

Background: Species of Echium trygorrhizum Pomel are used traditionally in Algeria folk medicine for the treatment of Diabetes, Jaundice and Tonsillitis. To our knowledge, no previous study has been conducted on the pharmacological activities of this species.

Objective: The objective of the present research was to evaluate the content of polyphenols, flavonoids and condensed tannins compounds and to assess in vitro the antioxidant activity and the inhibitory effect of the hydroalcoholic extract of this plant on α-amylase activity, an enzyme responsible for digestion of carbohydrate before the process of intestinal absorption.

Methods: Polyphenols, flavonoids and condensed tannins were evaluated spectrophotometrically using Folin-Ciocalteu, the Aluminum chloride and vanillin methods, respectively. The antioxidant activity using DPPH radical scavenging, ABTS, Ferric reducing antioxidant power and β-carotene bleaching tests and the assessment of in vitro α-amylase inhibitory potential by an Aspergillus oryzae α- amylase have been studied.

Results: The hydroalcoholic crude extract was able to inhibit the α-amylase enzyme in vitro, with an IC50 of 0.56 ± 0.044 mg/ml, in addition, the contents of polyphenols and flavonoids were found to be 157.403 ± 0.835 μg GAE/mg extract and 30.156±2.634μg Q E / mg extract, respectively, Whereas the amount of tannins was 65.293 ± 0.883μg Cat E/ mg of dry extract.

Conclusion: The present study revealed that the extract is rich in phenolic compounds, which play a really important role in the scavenging of free radicals. The inhibitory capacity of hydroalcoholic roots extract on Aspergillus oryzae α-amylase enzyme has been reported and might be used as a natural agent within the management of diabetes mellitus.

Keywords: Total phenols, flavonoids, tannins, antioxidant activity, anti-diabetic activity, α-amylase.

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