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Current Nutrition & Food Science

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ISSN (Print): 1573-4013
ISSN (Online): 2212-3881

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Research Article

Is Sunflower Cooking Oil Beneficial for Colorectal Cancer? In Vivo Studies on Azoxymethane-induced Colon Cancer in Rats

Author(s): Sheba R. Nakka David, Miza Syazwina Mohammad, Lim Ya Chee and Rajan Rajabalaya*

Volume 18, Issue 3, 2022

Published on: 19 January, 2022

Page: [329 - 336] Pages: 8

DOI: 10.2174/1573401317666211208144625

Price: $65

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Abstract

Background: The incorporation of oils in the diet may have promoting or inhibitory effects on Colorectal Cancer (CRC). In this study, azoxymethane (AOM) was used to mimic CRC in rats, and the effect of sunflower oil on cancer progression in the colon of the rats was tested.

Objective: This study was conducted to investigate the effect of sunflower oil on preneoplastic cancer properties of the colonic mucosal surface of tumors and the aberrant crypt foci (ACF).

Methods: Six-week-old Sprague-Dawley male rats were randomized into 4 groups of 6 rats each, namely naïve, positive control, negative control, and sunflower oil-fed. CRC was induced by AOM by subcutaneous injection of 20 mg/kg. After CRC induction, the rats were given the respective treatment of either basal diet (naïve group), 10 mg/kg indomethacin (positive control), 0.9 % saline (negative control), or 7 % sunflower oil (experimental group) daily by oral gavage for 42 days. Rats were sacrificed by cervical dislocation; colon samples were visually observed for any tumors on the colonic mucosal surface and evaluated for ACF; histopathological examinations were also performed.

Results: The mean body weights of the rats were similar in all groups as per one-way ANOVA. A total of 3 ACF were found in the negative group, while none were observed in other groups. The crypts appeared regular with circular luminal openings and were closely packed together in the naïve group. Crypts in the positive and treated group had a similar appearance to the naïve group.

Conclusion: Sunflower oil inhibition of the preneoplastic cancer ACF properties were tested but were found to be insignificant when administered during CRC treatment or management. However, long-term experiments with a greater number of days will yield better development of tumor and ACF development and will be useful in identifying the molecular mechanism.

Keywords: Preneoplastic, sunflower oil, aberrant crypt foci, azoxymethane, colorectal, cancer, cooking oil.

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[1]
Haggar FA, Boushey RP. Colorectal cancer epidemiology: Incidence, mortality, survival, and risk factors. Clin Colon Rectal Surg 2009; 6: 191-7.
[http://dx.doi.org/10.1055/s-0029-1242458]
[2]
Gandomani HS, Yousefi SM, Aghajani M, Mohammadian-Hafshejani A, Tarazoj AA, Pouyesh V. Colorectal cancer in the world: Incidence, mortality and risk factors. Biomed Res Ther 2017; 4: 1656.
[http://dx.doi.org/10.15419/bmrat.v4i10.372]
[3]
Simon K. Colorectal cancer development and advances in screening. Clin Interv Aging 2016; 11: 967-76.
[http://dx.doi.org/10.2147/CIA.S109285] [PMID: 27486317]
[4]
Valastyan S, Weinberg RA. Tumor metastasis: Molecular insights and evolving paradigms. Cell 2011; 147(2): 275-92.
[http://dx.doi.org/10.1016/j.cell.2011.09.024] [PMID: 22000009]
[5]
Roncucci L, Modica S, Pedroni M, et al. Aberrant crypt foci in patients with colorectal cancer. Br J Cancer 1998; 77(12): 2343-8.
[http://dx.doi.org/10.1038/bjc.1998.389] [PMID: 9649156]
[6]
Lijinsky W, Saavedra JE, Reuber MD. Organ-specific carcinogenesis in rats by methyl- and ethylazoxyalkanes. Cancer Res 1985; 45(1): 76-9.
[PMID: 3965154]
[7]
Orlando FA, Tan D, Baltodano JD, et al. Aberrant crypt foci as precursors in colorectal cancer progression. J Surg Oncol 2008; 98(3): 207-13.
[http://dx.doi.org/10.1002/jso.21106] [PMID: 18623110]
[8]
Doi K, Fujioka M, Sokuza Y, Ohnishi M, Gi M, Takeshita M. Chemopreventive action by ethanol-extracted Brazilian green propolis on post-initiation phase of inflammation-associated rat colon tumorigenesis.In Vivo (Brooklyn) 2017; 31: 187-97.
[http://dx.doi.org/10.21873/invivo.11044]
[9]
Berkel H, Holcombe RF, Middlebrooks M, Kannan K. Nonsteroidal antiinflammatory drugs and colorectal cancer. Epidemiol Rev 1996; 18(2): 205-17.
[http://dx.doi.org/10.1093/oxfordjournals.epirev.a017926] [PMID: 9021313]
[10]
Kudo T, Narisawa T, Abo S. Antitumor activity of indomethacin on methylazoxymethanol-induced large bowel tumors in rats. Gann 1980; 71(2): 260-4.
[PMID: 7202921]
[11]
Narisawa T, Sato M, Tani M, Kudo T, Takahashi T, Goto A. Inhibition of development of methylnitrosourea-induced rat colon tumors by indomethacin treatment. Cancer Res 1981; 41(5): 1954-7.
[PMID: 7214363]
[12]
Pollard M, Luckert PH, Schmidt MA. The suppressive effect of piroxicam on autochthonous intestinal tumors in the rat. Cancer Lett 1983; 21(1): 57-61.
[http://dx.doi.org/10.1016/0304-3835(83)90082-4] [PMID: 6640513]
[13]
Ryan-Harshman M, Aldoori W. Diet and colorectal cancer: Review of the evidence. Can Fam Physician 2007; 53(11): 1913-20.
[http://dx.doi.org/10.1186/cc1879] [PMID: 18000268]
[14]
Panala V, Verghese M, Boateng J, Field R, Shackelford L, Walker LT. A comparison of rice bran, corn oil and soybean oil against azoxymethane induced colon cancer in a fisher 344 rat model. Int J Cancer Res 2009; 5: 25-35.
[http://dx.doi.org/10.3923/ijcr.2009.25.35]
[15]
Fujise T, Iwakiri R, Kakimoto T, et al. Long-term feeding of various fat diets modulates azoxymethane-induced colon carcinogenesis through Wnt/beta-catenin signaling in rats. Am J Physiol Gastrointest Liver Physiol 2007; 292(4): G1150-6.
[http://dx.doi.org/10.1152/ajpgi.00269.2006] [PMID: 17194898]
[16]
Bartolí R, Navarro E, Castellà E, Mañé J, Alvarez M, Pastor C. Effect of olive oil on early and late events of colon carcinogenesis in rats: Modulation of arachidonic acid metabolism and local prostaglandin E2 synthesis. Gut 2000; 46(2): 191-9.
[http://dx.doi.org/10.1136/gut.46.2.191]
[17]
Thomas A, Matthäus B, Fiebig H-J. Fats and Fatty Oils. In: Ullmann’s Encycl Ind Chem Weinheim, Germany: Wiley-VCH Verlag GmbH & Co KGaA. 2015; pp. 1-84.
[http://dx.doi.org/10.1002/14356007.a10_173.pub2]
[18]
Pericleous M, Mandair D, Caplin ME. Diet and supplements and their impact on colorectal cancer. J Gastrointest Oncol 2013; 4(4): 409-23.
[http://dx.doi.org/10.3978/j.issn.2078-6891.2013.003] [PMID: 24294513]
[19]
Pédeboscq S, Rey C, Petit M, et al. Non-antioxidant properties of α-tocopherol reduce the anticancer activity of several protein kinase inhibitors in vitro. PLoS One 2012; 7(5): e36811.
[http://dx.doi.org/10.1371/journal.pone.0036811] [PMID: 22590613]
[20]
Ju J, Picinich SC, Yang Z, et al. Cancer-preventive activities of tocopherols and tocotrienols. Carcinogenesis 2010; 31(4): 533-42.
[http://dx.doi.org/10.1093/carcin/bgp205] [PMID: 19748925]
[21]
Caderni G, Bollito E, Tessitore L. Colon cancer is induced by a single low dose of azoxymethane in fasted-refed rats. Nutr Cancer 1999; 35(2): 137-42.
[http://dx.doi.org/10.1207/S15327914NC352_7] [PMID: 10693167]
[22]
National Research Council (US) Subcommittee on Laboratory Animal Nutrition. Nutrient Requeriment of Laboratory Animals. 4th ed. Washington, D.C: The National Academy of Sciences, National Academy Press 1995.
[23]
Carbone L, Carbone ET, Yi EM, et al. Assessing cervical dislocation as a humane euthanasia method in mice. J Am Assoc Lab Anim Sci 2012; 51(3): 352-6.
[PMID: 22776194]
[24]
Fleming M, Ravula S, Tatishchev SF, Wang HL. Colorectal carcinoma: Pathologic aspects. J Gastrointest Oncol 2012; 3(3): 153-73.
[http://dx.doi.org/10.3978/j.issn.2078-6891.2012.030] [PMID: 22943008]
[25]
Norazalina S, Norhaizan ME, Hairuszah I, Norashareena MS. Anticarcinogenic efficacy of phytic acid extracted from rice bran on azoxymethane-induced colon carcinogenesis in rats. Exp Toxicol Pathol 2010; 62(3): 259-68.
[http://dx.doi.org/10.1016/j.etp.2009.04.002] [PMID: 19464858]
[26]
Banerjee N, Kim H, Talcott S, Mertens-Talcott S. Pomegranate polyphenolics suppressed azoxymethane-induced colorectal aberrant crypt foci and inflammation: Possible role of miR-126/VCAM-1 and miR-126/PI3K/AKT/mTOR. Carcinogenesis 2013; 34(12): 2814-22.
[http://dx.doi.org/10.1093/carcin/bgt295] [PMID: 23996930]
[27]
Rao CV, Hirose Y, Indranie C, Reddy BS. Modulation of experimental colon tumorigenesis by types and amounts of dietary fatty acids. Cancer Res 2001; 61(5): 1927-33.
[PMID: 11280748]
[28]
Williams D, Verghese M, Walker LT, Boateng J, Shackelford L, Chawan CB. Flax seed oil and flax seed meal reduce the formation of Aberrant Crypt Foci (ACF) in azoxymethane-induced colon cancer in Fisher 344 male rats. Food Chem Toxicol 2007; 45(1): 153-9.
[http://dx.doi.org/10.1016/j.fct.2006.08.014] [PMID: 17045379]
[29]
Kishimoto Y, Yashima K, Morisawa T, et al. Effects of long-term administration of sulindac on APC mRNA and apoptosis in colons of rats treated with azoxymethane. J Cancer Res Clin Oncol 2002; 128(11): 589-95.
[http://dx.doi.org/10.1007/s00432-002-0384-8] [PMID: 12458338]
[30]
Kohno H, Suzuki R, Yasui Y, Hosokawa M, Miyashita K, Tanaka T. Pomegranate seed oil rich in conjugated linolenic acid suppresses chemically induced colon carcinogenesis in rats. Cancer Sci 2004; 95(6): 481-6.
[http://dx.doi.org/10.1111/j.1349-7006.2004.tb03236.x] [PMID: 15182427]
[31]
Parnaud G, Taché S, Peiffer G, Corpet DE. Polyethylene-glycol suppresses colon cancer and causes dose-dependent regression of azoxymethane-induced aberrant crypt foci in rats. Cancer Res 1999; 59(20): 5143-7.
[PMID: 10537289]
[32]
Chen J, Huang XF. The signal pathways in azoxymethane-induced colon cancer and preventive implications. Cancer Biol Ther 2009; 8(14): 1313-7.
[http://dx.doi.org/10.4161/cbt.8.14.8983] [PMID: 19502780]

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