Login Register Cart 0
Research Article

Del-1诱导银屑病内皮细胞αvβ3和α5β1表达

卷 22, 期 5, 2022

发表于: 20 August, 2021

页: [442 - 448] 页: 7

弟呕挨: 10.2174/1566524021666210729112700

价格: $65

conference banner
摘要

目的：银屑病是一种高度依赖血管生成的慢性炎症性皮肤病。我们先前的研究结果表明，银屑病患者真皮间充质干细胞（dMSCs）中Del-1的mRNA和蛋白表达上调。我们的目的是进一步研究来自dMSCs的Del-1在银屑病发病机制中的作用，并证实Del-1在银屑病发病机制中的作用。 方法：我们进行了一项免疫组织化学实验，以进一步研究Del-1在银屑病患者皮损中的表达。此外，通过Del-1慢病毒载体将Del-1过表达的dMSCs与ECs共培养，通过蛋白质印迹法检测ECs整合素（αvβ3、αvβ5和α5β1）的蛋白表达。 结果：本研究表明，银屑病患者皮损中的Del-1显著增加（p<0.05，9.96 vs.2.18），dMSCs中的Del-1成功诱导整合素α5β1和αvβ3的上调（均p<0.05）。 结论：本研究阐述了dMSCs中的Del-1通过诱导血管生成参与银屑病的发病机制。Del-1、αvβ3和α5β1可能是抑制银屑病血管生成的潜在新靶点。

关键词: 银屑病，Del-1，真皮间充质干细胞（dMSCs），整合素，血管生成，蛋白质印迹法。

« Previous Next »
[1]
Ho HK, Jang JJ, Kaji S, et al. Developmental endothelial locus-1 (Del-1), a novel angiogenic protein: Its role in ischemia. Circulation 2004; 109(10): 1314-9.
[http://dx.doi.org/10.1161/01.CIR.0000118465.36018.2D] [PMID: 14981004]
[2]
Shin J, Hosur KB, Pyaram K, et al. Expression and function of the homeostatic molecule Del-1 in endothelial cells and the periodontal tissue. Clin Dev Immunol 2013; 2013617809
[http://dx.doi.org/10.1155/2013/617809] [PMID: 24416060]
[3]
Wang Z, Boyko T, Tran MC, et al. DEL1 protects against chondrocyte apoptosis through integrin binding. J Surg Res 2018; 231: 1-9.
[http://dx.doi.org/10.1016/j.jss.2018.04.066] [PMID: 30278915]
[4]
Penta K, Varner JA, Liaw L, Hidai C, Schatzman R, Quertermous T. Del1 induces integrin signaling and angiogenesis by ligation of alphaVbeta3. J Biol Chem 1999; 274(16): 11101-9.
[http://dx.doi.org/10.1074/jbc.274.16.11101] [PMID: 10196194]
[5]
Ciucurel EC, Sefton MV. Del-1 overexpression in endothelial cells increases vascular density in tissue-engineered implants containing endothelial cells and adipose-derived mesenchymal stromal cells. Tissue Eng Part A 2014; 20(7-8): 1235-52.
[http://dx.doi.org/10.1089/ten.tea.2013.0242] [PMID: 24151812]
[6]
Hidai C, Zupancic T, Penta K, et al. Cloning and characterization of developmental endothelial locus-1: An embryonic endothelial cell protein that binds the alphavbeta3 integrin receptor. Genes Dev 1998; 12(1): 21-33.
[http://dx.doi.org/10.1101/gad.12.1.21] [PMID: 9420328]
[7]
Takada Y, Ye X, Simon S. The integrins. Genome Biol 2007; 8(5): 215.
[http://dx.doi.org/10.1186/gb-2007-8-5-215] [PMID: 17543136]
[8]
Rocha LA, Learmonth DA, Sousa RA, Salgado AJ. αvβ3 and α5β1 integrin-specific ligands: From tumor angiogenesis inhibitors to vascularization promoters in regenerative medicine? Biotechnol Adv 2018; 36(1): 208-27.
[http://dx.doi.org/10.1016/j.biotechadv.2017.11.004] [PMID: 29155160]
[9]
Boudreau NJ, Varner JA. The homeobox transcription factor Hox D3 promotes integrin alpha5beta1 expression and function during angiogenesis. J Biol Chem 2004; 279(6): 4862-8.
[http://dx.doi.org/10.1074/jbc.M305190200] [PMID: 14610084]
[10]
McFadden J, Fry L, Powles AV, Kimber I. Concepts in psoriasis: Psoriasis and the extracellular matrix. Br J Dermatol 2012; 167(5): 980-6.
[http://dx.doi.org/10.1111/j.1365-2133.2012.11149.x] [PMID: 22803790]
[11]
Zhong J, Eliceiri B, Stupack D, et al. Neovascularization of ischemic tissues by gene delivery of the extracellular matrix protein Del-1. J Clin Invest 2003; 112(1): 30-41.
[http://dx.doi.org/10.1172/JCI17034] [PMID: 12840057]
[12]
Nickoloff BJ. Characterization of lymphocyte-dependent angiogenesis using a SCID mouse: Human skin model of psoriasis. J Investig Dermatol Symp Proc 2000; 5(1): 67-73.
[http://dx.doi.org/10.1046/j.1087-0024.2000.00006.x] [PMID: 11147678]
[13]
Fan Y, Zhu W, Yang M, et al. Del-1 gene transfer induces cerebral angiogenesis in mice. Brain Res 2008; 1219: 1-7.
[http://dx.doi.org/10.1016/j.brainres.2008.05.003] [PMID: 18534562]
[14]
Jeong D, Ban S, Oh S, Jin Lee S, Yong Park S, Koh YW. Prognostic significance of edil3 expression and correlation with mesenchymal phenotype and microvessel density in lung adenocarcinoma. Sci Rep 2017; 7(1): 8649.
[http://dx.doi.org/10.1038/s41598-017-08851-9] [PMID: 28819306]
[15]
Niu X, Chang W, Liu R, et al. mRNA and protein expression of the angiogenesis-related genes EDIL3, AMOT and ECM1 in mesenchymal stem cells in psoriatic dermis. Clin Exp Dermatol 2016; 41(5): 533-40.
[http://dx.doi.org/10.1111/ced.12783] [PMID: 26644074]
[16]
Creamer D, Sullivan D, Bicknell R, Barker J. Angiogenesis in psoriasis. Angiogenesis 2002; 5(4): 231-6.
[http://dx.doi.org/10.1023/A:1024515517623] [PMID: 12906316]
[17]
Teoh CM, Tan SSL, Tran T. Integrins as therapeutic targets for respiratory diseases. Curr Mol Med 2015; 15(8): 714-34.
[http://dx.doi.org/10.2174/1566524015666150921105339] [PMID: 26391549]
[18]
Matsushita J, Inagaki S, Nishie T, et al. Fluorescence and bioluminescence imaging of angiogenesis in flk1-nano-lantern transgenic mice. Sci Rep 2017; 7: 46597.
[http://dx.doi.org/10.1038/srep46597] [PMID: 28425492]
[19]
Hou R, Yin G, An P, et al. DNA methylation of dermal MSCs in psoriasis: Identification of epigenetically dysregulated genes. J Dermatol Sci 2013; 72(2): 103-9.
[http://dx.doi.org/10.1016/j.jdermsci.2013.07.002] [PMID: 23916410]
[20]
Ryu YH, Lee YJ, Kim KJ, Lee SJ, Han YN, Rhie JW. Epidermal growth factor (egf)-like repeats and discoidin i-like domains 3 (EDIL3): A potential new therapeutic tool for the treatment of keloid scars. Tissue Eng Regen Med 2017; 14(3): 267-77.
[http://dx.doi.org/10.1007/s13770-017-0034-5] [PMID: 30603483]
[21]
Zou X, Qiao H, Jiang X, Dong X, Jiang H, Sun X. Downregulation of developmentally regulated endothelial cell locus-1 inhibits the growth of colon cancer. J Biomed Sci 2009; 16: 33.
[http://dx.doi.org/10.1186/1423-0127-16-33] [PMID: 19292890]
[22]
Klotzsche-von Ameln A, Cremer S, Hoffmann J, et al. Endogenous developmental endothelial locus-1 limits ischaemia-related angiogenesis by blocking inflammation. Thromb Haemost 2017; 117(6): 1150-63.
[http://dx.doi.org/10.1160/TH16-05-0354] [PMID: 28447099]
[23]
Orciani M, Campanati A, Salvolini E, et al. The mesenchymal stem cell profile in psoriasis. Br J Dermatol 2011; 165(3): 585-92.
[http://dx.doi.org/10.1111/j.1365-2133.2011.10438.x] [PMID: 21623755]
[24]
Creamer D, Allen M, Sousa A, Poston R, Barker J. Altered vascular endothelium integrin expression in psoriasis. Am J Pathol 1995; 147(6): 1661-7.
[PMID: 7495291]
[25]
Takeshita J, Grewal S, Langan SM, et al. Psoriasis and comorbid diseases: Epidemiology. J Am Acad Dermatol 2017; 76(3): 377-90.
[http://dx.doi.org/10.1016/j.jaad.2016.07.064] [PMID: 28212759]

Rights & Permissions Print Cite
Article Metrics
23
1
© 2024 Bentham Science Publishers | Privacy Policy