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Research Article

基于TGF-β1的CRISPR/Cas9基因治疗可减轻辐射诱导的肺损伤

卷 22, 期 1, 2022

发表于: 30 December, 2020

页: [59 - 65] 页: 7

弟呕挨: 10.2174/1566523220666201230100523

价格: $65

摘要

背景:放射性肺损伤(RILI)缺乏有效的治疗策略。在本研究中，我们对RILI进行了基于TGF-β1的CRISPR/Cas9基因治疗。目的:用单剂量20gy γ射线照射小鼠肺，然后静脉注射Ad- crispr - tgf -β1或Ad- CRISPR-Null。 方法:采用苏木素、伊红染色和马松染色观察肺组织形态。采用ELISA法测定支气管肺泡灌洗液中白蛋白和IgM的浓度。用ELISA和/或real-time PCR检测细胞因子水平，并采用末端脱氧核苷酸转移酶介导的末端标记。 结果:Ad-CRISPR-TGF-β1改善肺损伤的组织病理学和生化指标，减少炎性细胞因子的分泌和表达，抑制纤维化的进展。重要的是，SK1/S1P轴通过S1P1在TGF-β1依赖的S1PR模式重构中发挥关键作用，并促进纤维化。 结论:我们的研究结果为RILI治疗提供了新的思路。

关键词: CRISPR/Cas9，纤维化，炎症，肺，基因治疗，CRISPR/Cas9。

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