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ISSN (Print): 2211-5501
ISSN (Online): 2211-551X

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Research Article

Screening and Optimization of Protease Enzyme Produced by Strains of Alkalihalobacillus Sp. and Bacillus Sp.

Author(s): Shirin Saberianpour, Leila Abkhooie, Babak Elyasifar and Azita Dilmaghani*

Volume 10, Issue 1, 2021

Published on: 02 December, 2020

Page: [40 - 45] Pages: 6

DOI: 10.2174/2211550109999201202123222

Price: $65

Abstract

Background: Proteases are the most important industrial enzymes with diverse applications in bacteria, such as Bacillus, commonly used to produce protease for industrial purposes. Proteases are commercially exploited in a larger scale, especially in pharmaceutical, food, leather, and detergent industries.

Objective: The present study sought to screen and optimize protease enzyme activity produced by local bacteria.

Methods: The effects of incubation time, temperature, and initial pH were investigated to improve the extracellular protease enzyme activity by two bacteria, named Bacillus subtilis strain DAR and Alkalihalobacillus hwajinpoensis strain 3NB. These two isolates have already been isolated and registered from Iran.

Results: The results indicated that the optimum incubation time for the protease activity in B. Subtilis strain DAR is 36 h in contrast to 40 h in Alkalihalobacillus hwajinpoensis strain 3NB. The optimum incubation temperatures for enzyme activity for B. Subtilis and Alkalihalobacillus hwajinpoensis are 50°C and 40°C, respectively. Optimum pH values for protease activity of both bacteria are 8.

Conclusion: The optimum incubation time, pH, and temperature were investigated for better protease activity. Further studies are recommended to improve protease activity by changing enzyme substrates.

Keywords: Protease, optimization, screening, Bacillus, local bacteria, Alkalihalobacillus.

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[1]
Rengasamy G, Jebaraj DM, Veeraraghavan VP, Krishna S. Characterization, Partial Purification of Alkaline Protease from Intestinal Waste of Scomberomorus Guttatus and Production of Laundry Detergent with Alkaline Protease Additive. Indian J Pharm Educ Res 2016; 50(2): S59-67.
[2]
DeSantis TZ, Dubosarskiy I, Murray SR, Andersen GL. Comprehensive aligned sequence construction for automated design of effective probes (CASCADE-P) using 16S rDNA. Bioinformatics 2003; 19(12): 1461-8.
[http://dx.doi.org/10.1093/bioinformatics/btg200] [PMID: 12912825]
[3]
Akcan N, Uyar F. Production of extracellular alkaline protease from Bacillus subtilis RSKK96 with solid state fermentation. EurAsian Journal of BioSciences 2011; 5.
[4]
Tarhriz V, Akbari Z, Dilmaghani A, Hamidi A, Hejazi MA, Hejazi MS. Bioreduction of iron and biosorption of heavy metals (Ni 2+, Co 2+, Pb 2+) by a novel environmental bacterium, Tabrizicola aquatica rcri19 t. Asian J Water Environ Pollut 2019; 16(3): 73-81.
[http://dx.doi.org/10.3233/AJW190035]
[5]
Abdehvand AZ, Keshtkar A, Fatemi F, Tarhriz V, Hejazi MS. Removal of U (VI) from aqueous solutions using Shewanella sp. RCRI7, isolated from Qurugöl Lake in Iran. Radiochim Acta 2017; 105(2): 109-20.
[http://dx.doi.org/10.1515/ract-2016-2628]
[6]
Tarhriz V, Hamidi A, Rahimi E, Eramabadi M, Eramabadi P, Yahaghi E, et al. Isolation and characterization of naphtalene-degradation bacteria from Qurugol lake located at Azerbaijan. Biosci Biotechnol Res Asia 2014; 11(2): 715-22.
[http://dx.doi.org/10.13005/bbra/1326]
[7]
Seyfi R, Kahaki FA, Ebrahimi T, Montazersaheb S, Eyvazi S, Babaeipour V, et al. Antimicrobial Peptides (AMPs): Roles, Functions and Mechanism of Action. Int J Pept Res Ther 2019; 1-13.
[http://dx.doi.org/10.1007/s10989-019-09946-9]
[8]
Tarhriz V, Eyvazi S, Shakeri E, Hejazi MS, Dilmaghani A. Antibacterial and antifungal activity of novel freshwater bacterium “tabrizicola aquatica “as a prominent natural antibiotic available in grügol lake Pharma Sci 2020; 6(1): 88-92.
[http://dx.doi.org/10.34172/PS.2019.56]
[9]
Elyasi Far B, Ahmadi Y, Yari Khosroshahi A, Dilmaghani A. Microbial alpha-amylase production: progress, challenges and perspectives. Adv Pharm Bull 2020; 10(3): 350-8.
[http://dx.doi.org/10.34172/apb.2020.043] [PMID: 32665893]
[10]
ARZITA A. KEANEKARAGAMAN Bacillus TERMOFILIK OBLIGAT DAN KERABATNYA PADA SUMBER AIR PANAS KERINCI JAMBI UNTUK MENGHASILKAN PROTEASE SERIN ALKALI YANG BERPOTENSI SEBAGAI ADITIF DETERJEN: Universitas Andalas 2018.
[11]
Yadav SK, Bisht D, Shikha S, Darmwal NS. Oxidant and solvent stable alkaline protease from Aspergillus flavus and its characterization. Afr J Biotechnol 2011; 10(43): 8630-40.
[http://dx.doi.org/10.5897/AJB10.1611]
[12]
Sevinc N, Demirkan E. Production of protease by Bacillus sp. N-40 isolated from soil and its enzymatic properties. J Biol Environ Sci 2011; 5(14)
[13]
Reddy LV, Wee Y-J, Yun J-S, Ryu H-W. Optimization of alkaline protease production by batch culture of Bacillus sp. RKY3 through Plackett-Burman and response surface methodological approaches. Bioresour Technol 2008; 99(7): 2242-9.
[http://dx.doi.org/10.1016/j.biortech.2007.05.006] [PMID: 17596938]
[14]
Chanalia P, Gandhi D, Jodha D, Singh J. Applications of microbial proteases in pharmaceutical industry: an overview. Rev Med Microbiol 2011; 22(4): 96-101.
[http://dx.doi.org/10.1097/MRM.0b013e3283494749]
[15]
Fath M, Fazaelipoor MH. Production of Proteases in a Novel Trickling Tray Bioreactor. Waste Biomass Valoriz 2015; 6(4): 475-80.
[http://dx.doi.org/10.1007/s12649-015-9371-6]
[16]
Ellaiah P, Srinivasulu B, Adinarayana K. A review on microbial alkaline proteases.J Sci Ind Res 2002; 61(9): 690-704.
[17]
Chu W-H. Optimization of extracellular alkaline protease production from species of Bacillus. J Ind Microbiol Biotechnol 2007; 34(3): 241-5.
[http://dx.doi.org/10.1007/s10295-006-0192-2] [PMID: 17171551]
[18]
Nijland R, Kuipers OP. Optimization of protein secretion by Bacillus subtilis. Recent Pat Biotechnol 2008; 2(2): 79-87.
[http://dx.doi.org/10.2174/187220808784619694] [PMID: 19075856]
[19]
Sierecka JK. Purification and partial characterization of a neutral protease from a virulent strain of Bacillus cereus. Int J Biochem Cell Biol 1998; 30(5): 579-95.
[http://dx.doi.org/10.1016/S1357-2725(98)00007-7] [PMID: 9693959]
[20]
Far BE, Khosroushahi AY, Dilmaghani A. In Silico Study of Alkaline Serine Protease and Production Optimization in Bacillus sp Khoz1 Closed Bacillus safensis Isolated from Honey Int J of Peptide Res Ther 2020; 1-11.
[21]
Yang J, Shih I, Tzeng Y, Wang S. Production and purification of protease from a Bacillus subtilis that can deproteinize crustacean wastes*. Enzyme Microb Technol 2000; 26(5-6): 406-13.
[http://dx.doi.org/10.1016/S0141-0229(99)00164-7] [PMID: 10713215]
[22]
Thangam EB, Rajkumar GS. Purification and characterization of alkaline protease from Alcaligenes faecalis. Biotechnol Appl Biochem 2002; 35(2): 149-54.
[http://dx.doi.org/10.1042/BA20010013] [PMID: 11916457]
[23]
Kalisz HM. Microbial proteinases Adv Biochem Eng Biotechnol 1988; 39: 1-65.
[24]
Shadi M, Heydari H, Elyasifar B, Dilmaghani A. Isolation and identification of hydrolytic enzymes generated by halophilic bacteria in center of important Med J Tabriz Univ Med Sci 2019; 41(5): 65-71.
[25]
Tarhriz V, Mohammadzadeh F, Hejazi MS, Nematzadeh G, Rahimi E. Isolation and characterization of some aquatic bacteria from Qurugol Lake in Azerbaijan under aerobic conditions. Adv Environ Biol 2011; 3173-9.
[26]
Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4(4): 406-25.
[PMID: 3447015]
[27]
Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35(6): 1547-9.
[http://dx.doi.org/10.1093/molbev/msy096] [PMID: 29722887]
[28]
Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 2004; 101(30): 11030-5.
[http://dx.doi.org/10.1073/pnas.0404206101] [PMID: 15258291]
[29]
Tarhriz V, Nouioui I, Spröer C, Verbarg S, Ebrahimi V, Cortés-Albayay C, et al. Pseudomonas khazarica sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from Khazar Sea sediments. Antonie van Leeuwenhoek 2019; 1-12.
[PMID: 31768782]
[30]
Chatterjee J, Giri S, Maity S, et al. Production and characterization of thermostable alkaline protease of Bacillus subtilis (ATCC 6633) from optimized solid-state fermentation. Biotechnol Appl Biochem 2015; 62(5): 709-18.
[http://dx.doi.org/10.1002/bab.1309] [PMID: 25323045]
[31]
Adinarayana K, Ellaiah P, Prasad DS. Purification and partial characterization of thermostable serine alkaline protease from a newly isolated Bacillus subtilis PE-11. AAPS PharmSciTech 2003; 4(4)E56.
[http://dx.doi.org/10.1208/pt040456] [PMID: 15198551]
[32]
Agrawal D, Patidar P, Banerjee T, Patil S. Production of alkaline protease by Penicillium sp. under SSF conditions and its application to soy protein hydrolysis. Process Biochem 2004; 39(8): 977-81.
[http://dx.doi.org/10.1016/S0032-9592(03)00212-7]
[33]
Woods RG, Burger M, Beven C-A, Beacham IR. The aprX-lipA operon of Pseudomonas fluorescens B52: a molecular analysis of metalloprotease and lipase production. Microbiology (Reading) 2001; 147(Pt 2): 345-54.
[http://dx.doi.org/10.1099/00221287-147-2-345] [PMID: 11158351]
[34]
Alves MP, Salgado RL, Eller MR, Dias RS, Oliveira de Paula S, Fernandes de Carvalho A. Temperature modulates the production and activity of a metalloprotease from Pseudomonas fluorescens 07A in milk. J Dairy Sci 2018; 101(2): 992-9.
[http://dx.doi.org/10.3168/jds.2017-13238] [PMID: 29248219]
[35]
Moorthy IMG, Baskar R. Statistical modeling and optimization of alkaline protease production from a newly isolated alkalophilic Bacillus species BGS using response surface methodology and genetic algorithm. Prep Biochem Biotechnol 2013; 43(3): 293-314.
[http://dx.doi.org/10.1080/10826068.2012.719850] [PMID: 23379276]
[36]
Das G, Prasad M. Isolation, purification & mass production of protease enzyme from Bacillus subtilis. Int Res J Microbiol 2010; 1(2): 26-31.
[37]
Akhavan Sepahy A, Jabalameli L. Effect of culture conditions on the production of an extracellular protease by Bacillus sp. isolated from soil sample of Lavizan Jungle Park. Enzyme research 2011 2011.219628..
[http://dx.doi.org/10.4061/2011/219628] [PMID: 22191016]

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