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General Research Article

间充质干细胞可通过GSK-3α/ β和ERK1/2信号通路促进Molt-4白血病细胞中Caspase的表达

卷 21, 期 1, 2021

发表于: 05 October, 2020

页: [81 - 88] 页: 8

弟呕挨: 10.2174/1566523220666201005111126

价格: $65

摘要

背景：间充质干细胞(MSCs)由于其独特的特性，如自我更新、多能性和多能性，被认为是一种有趣的细胞治疗工具。这些细胞的多功能特性正在许多研究中被研究。本研究考察了MSCs对急性淋巴细胞白血病(ALL)细胞Molt-4细胞系的影响。 方法：MSCs在trans-well系统中培养、表征并与Molt-4细胞共培养。第7天收集Molt-4单独培养和Molt-4与MSCs(10:1)共培养，western blotting检测蛋白表达。分别用PCR-ELISA TRAP法和β-半乳糖苷酶活性测定端粒酶活性和细胞衰老。 结果：我们发现，MSCs导致pro-caspase-8和cleaved-caspase-8和9表达水平显著增加。GSK-3α/β和ERK1/2蛋白表达水平显著降低。MSCs使Molt-4细胞端粒酶活性显著降低，β-半乳糖苷酶活性显著升高 结论：由此可见，MSCs与Molt-4细胞共培养可通过caspase-8、9级联及GSK-3α/β和ERK1/2信号通路促进Molt-4细胞凋亡和细胞衰老。

关键词: 间充质干细胞，caspase 8和9，凋亡，GSK-3α/β和ERK1/2信号通路，干细胞基础治疗

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