Abstract
Background: Protium heptaphyllum (Aubl.) March resin is accepted as an analgesic, healing, expectorant, anti-inflammatory, antinociceptive and gastroprotective agent. However, there are no specific studies on leaves of this plant.
Objective: This study identified the chemical composition and biological activity of crude ethanolic extract (EE) and ethyl acetate (EA) fraction from P. heptaphyllum.
Methods: Mice were intoxicated with acetaminophen (PCM; 250 mg kg-1), and subsequently treated with EE and EA (100 mg kg-1) for 7 days via gavage. Plasma analysis and evaluation of liver and kidney homogenates of the animals were performed.
Results: The extract and fraction showed the presence of phenols and flavonoids. Three main flavonoids were identified by HPLC-UV, LC-MS/MS fractionation, quercetin-3-β-D-glycoside, myricetin and quercetin. For total antioxidant potential tests, EE presented EC50 of 75 mg kg-1 and 100 μg mL-1 for EA. ALT, AST and ALP enzymes activity increased in the PCM-exposed plasma, but EA decreased these activities at control levels for ALT and AST. Animal glycemic levels decreased with EE and EA, which may be due to a possible hypoglycemic effect of the plant. In the liver tissue, SOD was unchanged, CAT, GSH and ASA decreased in the PCM group, but EA was able to reverse these effects. TBARS and PC were reduced by both treatments. In the renal tissue, EA fraction recovered the GST activity, GSH levels were restored by EE and EA and PC was reduced by treatments.
Conclusion: This antioxidant effect may be due to the presence of the flavonoids identified, which are already well known for their antioxidant properties.
Keywords: Acetaminophen, biomarker, free radical, intoxication, hepatotoxicity, oxidative stress.
Graphical Abstract
The Natural Products Journal
Title:Identification of Flavonoids by LC-MS/MS in Leaves Extract From Protium heptaphyllum (Aubl.) March and Antioxidant Activity in Mice
Volume: 11 Issue: 5
Author(s): Naiéle Sartori Patias, Valéria Dornelles Gindri Sinhorin*, Fernando Rafael de Moura, Ana Paula Simóes da Cunha, Ritane Rose da Silva Lima, Thaniara Barbosa da Costa, Romeu José da Costa, Larissa Cavalheiro, Rogério de Campos Bicudo and Adilson Paulo Sinhorin
Affiliation:
- Programa de Pos Graduacao em Ciencias Ambientais, Instituto de Ciencias Naturais, Humanas e Sociais, Universidade Federal de Mato Grosso, Sinop,Brazil
Keywords: Acetaminophen, biomarker, free radical, intoxication, hepatotoxicity, oxidative stress.
Abstract:
Background: Protium heptaphyllum (Aubl.) March resin is accepted as an analgesic, healing, expectorant, anti-inflammatory, antinociceptive and gastroprotective agent. However, there are no specific studies on leaves of this plant.
Objective: This study identified the chemical composition and biological activity of crude ethanolic extract (EE) and ethyl acetate (EA) fraction from P. heptaphyllum.
Methods: Mice were intoxicated with acetaminophen (PCM; 250 mg kg-1), and subsequently treated with EE and EA (100 mg kg-1) for 7 days via gavage. Plasma analysis and evaluation of liver and kidney homogenates of the animals were performed.
Results: The extract and fraction showed the presence of phenols and flavonoids. Three main flavonoids were identified by HPLC-UV, LC-MS/MS fractionation, quercetin-3-β-D-glycoside, myricetin and quercetin. For total antioxidant potential tests, EE presented EC50 of 75 mg kg-1 and 100 μg mL-1 for EA. ALT, AST and ALP enzymes activity increased in the PCM-exposed plasma, but EA decreased these activities at control levels for ALT and AST. Animal glycemic levels decreased with EE and EA, which may be due to a possible hypoglycemic effect of the plant. In the liver tissue, SOD was unchanged, CAT, GSH and ASA decreased in the PCM group, but EA was able to reverse these effects. TBARS and PC were reduced by both treatments. In the renal tissue, EA fraction recovered the GST activity, GSH levels were restored by EE and EA and PC was reduced by treatments.
Conclusion: This antioxidant effect may be due to the presence of the flavonoids identified, which are already well known for their antioxidant properties.
Export Options
About this article
Cite this article as:
Patias Sartori Naiéle , Gindri Sinhorin Dornelles Valéria *, de Moura Rafael Fernando , da Cunha Paula Simóes Ana , da Silva Lima Rose Ritane , da Costa Barbosa Thaniara , da Costa José Romeu , Cavalheiro Larissa , de Campos Bicudo Rogério and Sinhorin Paulo Adilson, Identification of Flavonoids by LC-MS/MS in Leaves Extract From Protium heptaphyllum (Aubl.) March and Antioxidant Activity in Mice, The Natural Products Journal 2021; 11 (5) . https://dx.doi.org/10.2174/2210315510999200817165311
DOI https://dx.doi.org/10.2174/2210315510999200817165311 |
Print ISSN 2210-3155 |
Publisher Name Bentham Science Publisher |
Online ISSN 2210-3163 |

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Inhibition of DNA Topoisomerases by a Series of Benzoxazoles and their Possible Metabolites
Letters in Drug Design & Discovery α7 Nicotinic Acetylcholine Receptors: A Therapeutic Target in the Structure Era
Current Drug Targets Radiolabeled Iron Oxide Nanoparticles As Dual-Modality SPECT/MRI and PET/MRI Agents
Current Topics in Medicinal Chemistry Targeted Drug Delivery: Trends and Perspectives
Current Drug Delivery Health Benefits of Manuka Honey as an Essential Constituent for Tissue Regeneration
Current Drug Metabolism First Synthesis of 5- and 7-Phenylethynyl-8-hydroxyquinolines by Sonogashira Reaction
Letters in Organic Chemistry Cratylia mollis Lectin: A Versatile Tool for Biomedical Studies
Current Bioactive Compounds From Drug Target to Leads-Sketching A Physicochemical Pathway for Lead Molecule Design In Silico
Current Pharmaceutical Design Evaluation of Fluorescence Resonance Energy Transfer Approaches as a Tool to Quantify the Stability of Antisense Oligodeoxynucleotides
Current Pharmaceutical Analysis Impact of Ritonavir, Atazanavir and Their Combination on the CYP3A4 Induction by Efavirenz in Primary Human Hepatocytes
Drug Metabolism Letters Non-cholesterol Sterols in the Diagnosis and Treatment of Dyslipidemias: A Review
Current Medicinal Chemistry Tracking Cell Signaling Protein Expression and Phosphorylation by Innovative Proteomic Solutions
Current Pharmaceutical Biotechnology Synthesis and Applications of Glyconanoparticles
Current Organic Chemistry Molecular Targeted Magnetic Resonance Imaging of Human Colorectal Carcinoma (LoVo) Cells Using Novel Superparamagnetic Iron Oxide- Loaded Nanovesicles: In Vitro and in vivo Studies
Current Cancer Drug Targets Evaluation of Drug Transporter Interactions in Drug Discovery and Development
Combinatorial Chemistry & High Throughput Screening The Screening of Renoprotective Agents by 99mTc-DMSA: A Review of Preclinical Studies
Current Radiopharmaceuticals Use of Mesenchymal Stem Cells in Crohn's Disease and Perianal Fistulas: A Narrative Review
Current Stem Cell Research & Therapy Drug-Drug Interactions: Antiretroviral Drugs and Recreational Drugs
Recent Patents on CNS Drug Discovery (Discontinued) Lycorine and its Derivatives for Anticancer Drug Design
Mini-Reviews in Medicinal Chemistry Recent Progress on Bioinformatics, Functional Genomics, and Metabolomics Research of Cytochrome P450 and its Impact on Drug Discovery
Current Topics in Medicinal Chemistry