Voltammetric Analysis of Ephedrine in Pharmaceutical Dosage Forms and Urine Using poly(Nile Blue A) Modified Glassy Carbon Electrode

Fatma       Ağın; Gökçe       Öztürk; Dilek       Kul
doi:10.2174/1386207323666200727100231
Abstract

Objective: The electrochemical analysis of ephedrine which is a sympathometric drug has been studied using poly(Nile blue A) modified glassy carbon electrodes, cyclic voltammetry, differential pulse voltammetry and square wave voltammetry.
Methods: The modified electrodes were prepared by potential cycling electropolymerization of Nile blue A in 0.1 M phosphate buffer solution at pH 6.0. The redox behavior of ephedrine was investigated in different buffer solutions at pH values between 5.5 and 9.0.
Results: Scan rate studies showed that the electron transfer reaction of ephedrine was diffusion controlled. A linear response was obtained between the peak current and the ephedrine concentration in the range of 0.6 to 100 μM with a limit of detection of 2.91×10^-3 μM for differential pulse voltammetry in Britton-Robinson buffer solution at pH 9.0. The linearity range of ephedrine in human urine was between 1.0 and 100 μM with a detection limit of 8.16 nM.
Conclusion: The recovery studies in both pharmaceutical dosage forms and urine showed that the proposed method ensured good selectivity, precision and accuracy without any interference from inactive excipients.
Keywords: Ephedrine, poly(Nile blue A), voltammetry, modified glassy carbon electrode, electropolymerization, pharmaceuticals.
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DOI https://dx.doi.org/10.2174/1386207323666200727100231	Print ISSN 1386-2073
Publisher Name Bentham Science Publisher	Online ISSN 1875-5402
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