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General Research Article

超声靶向微泡破坏转染TGF- shRNA，在体内外抑制大鼠跟腱损伤早期黏附修复

卷 20, 期 1, 2020

页: [71 - 81] 页: 11

弟呕挨: 10.2174/1566523220666200516165828

价格: $65

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摘要

背景:肌腱损伤是一种主要的骨科疾病。超声靶向微泡破坏(UTMD)是一种很有前途的基因转染方法，可用于肌腱损伤的治疗 目的:利用UTMD体外研究最佳转化生长因子(TGF- macrophach)短发夹RNA (shRNA)序列和转染条件，并在体内鉴定其对大鼠跟腱损伤早期黏附修复的抑制能力。 方法:选择最佳序列，荧光显微镜下分析，体外实时定量逆转录聚合酶链反应。将40只跟腱损伤大鼠在体内分为5组:(1)对照组，(2)质粒组，(3)质粒+超声组，(4)质粒+微泡组，(5)质粒+微泡+超声组，治疗14天后安乐死。通过黏附评分和病理检查评估TGF-表达。 结果:体外给药的最佳条件为输出强度为1W/cm2，占空比为30%，照射时间为60 s (P <0.05)。5组质粒转染效率高于其他组(P <0.05)。5组黏附指数评分最低，TGF-表达最少(P <0.05)。与其他组相比，第5组的炎症反应较轻。 结论:UTMD传递TGF- shRNA是治疗体内肌腱损伤的一种有效方法。

关键词: 超声靶向微泡破环

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