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Infectious Disorders - Drug Targets

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ISSN (Print): 1871-5265
ISSN (Online): 2212-3989

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Research Article

Evaluation of Glutathione Bioactivity on Toxoplasma Gondii in BALB/c Mice Post Impact of Selenium and Calcium Supplementation

Author(s): Mohammad Javad Namazi, Saeideh Nikpour, Parvaneh Rahimi-Moghaddam, Seyed Abdollah Hosseini, Abolghasem Siyadatpanah, Neda Sadat Shokouhi Mostafavi, Rahim Golmohammadi and Fatemeh Tabatabaie*

Volume 21, Issue 3, 2021

Published on: 16 May, 2020

Page: [452 - 458] Pages: 7

DOI: 10.2174/1871526520666200516161356

Price: $65

Abstract

Background: Studies have shown that selenium is an essential component of glutathione as an important antioxidant to reduce oxidative stress and inhibit intracellular parasites’ growth. In contrast, calcium in the cytosol of such parasites plays a key role in the entry of the parasite into the host cell and its primary motility.

Aims and Objectives: The present study was designed to evaluate and compare glutathione peroxidase bioactivity effects post administration of selenium and calcium in BALB/c mice infected by Toxoplasma gondii.

Methods: Sixty BALB/c mice susceptible to T. gondii were randomly divided into twelve groups of case and control groups. There were six control groups including two positive controls infected only with the parasites either 104 or 5×104, non-infected and untreated groups. Treated controls received only calcium, selenium, or both respectively. Case groups were infected with 104 or 5×104 parasites. While each set of three case groups separately received minerals alone or together. Mice were orally fed with 200 μg selenium, 50 μg calcium or their combination for 7 days. Mice were infected by parasite’s tachyzoites. Sera of mice were kept and the peritoneal macrophages were isolated for counting tachyzoites during infection.

Results: The results showed that selenium unlike calcium was significantly effective in reducing Toxoplasma tachyzoites compared to control groups. Moreover, glutathione peroxidase [GPX] activity was elevated in mice treated with selenium and vice versa decreased in mice treated with calcium.

Conclusion: Administration of selenium unlike calcium reduced Toxoplasma tachyzoites proliferation by elevating bioactivity of selenium-dependent detoxification enzyme, GPX.

Keywords: Toxoplama gondii, Selenium, Glutathione Peroxidase, BALB/c Mice, Calcium.

Graphical Abstract

[1]
Holland Alday, P; Stone Doggett, J. Drugs in development for toxoplasmosis: advances, challenges, and current status. 2017, 11, 273-293.
[2]
Dong, H.; Su, R.; Lu, Y.; Wang, M.; Liu, J.; Jian, F.; Yang, Y. Prevalence, risk factors, and genotypes of Toxoplasma gondii in food animals and humans [2000–2017] from China. Front. Microbiol., 2018, 9, 2108.
[http://dx.doi.org/10.3389/fmicb.2018.02108] [PMID: 30254613]
[3]
Wilking, H.; Thamm, M.; Stark, K.; Aebischer, T.; Seeber, F. Prevalence, incidence estimations, and risk factors of Toxoplasma gondii infection in Germany: a representative, cross-sectional, serological study. Sci. Rep., 2016, 6, 22551.
[http://dx.doi.org/10.1038/srep22551] [PMID: 26936108]
[4]
Walle, F.; Kebede, N.; Tsegaye, A.; Kassa, T. Seroprevalence and risk factors for Toxoplasmosis in HIV infected and non-infected individuals in Bahir Dar, Northwest Ethiopia. Parasit Vectors, 2013, 6(1), 15.
[http://dx.doi.org/10.1186/1756-3305-6-15] [PMID: 23324409]
[5]
Chaudhry, S.A.; Gad, N.; Koren, G. Toxoplasmosis and pregnancy. Can. Fam. Physician, 2014, 60(4), 334-336.
[PMID: 24733322]
[6]
Ebrahim Zadeh, A.; Bamedi, T.; Etemadi, S.; Shahrakipour, M.; Saryazdipour, Kh. Toxoplasmosis as a complication of transfusion in hemodialysis patients. Iran. J. Ped. Hematol. Oncol., 2014, 4(1), 22-25.
[PMID: 24734160]
[7]
Razzak, A.H.; Wais, S.A.; Saeid, A.Y. Toxoplasmosis: the innocent suspect of pregnancy wastage in Duhok, Iraq. East. Mediterr. Health J., 2005, 11(4), 625-632.
[PMID: 16700377]
[8]
Daryani, A.; Sagha, M. Seroepidemiology of Toxoplasmosis in women referred to medical health laboratory before marriage, Ardebil. J Ardabil Univ Med Sci., 2004, 4(13), 6.
[9]
Topley, WWC; Wilson, SGS; Collier, LH; Mahy, BW; Ter Meulen, V Topley & Wilson's microbiology and microbial infections: Hodder Arnold, 2005.
[10]
Gerhold, R.W.; Saraf, P.; Chapman, A.; Zou, X.; Hickling, G.; Stiver, W.H.; Houston, A.; Souza, M.; Su, C. Toxoplasma gondii seroprevalence and genotype diversity in select wildlife species from the southeastern United States. Parasit. Vectors, 2017, 10(1), 508.
[http://dx.doi.org/10.1186/s13071-017-2456-2] [PMID: 29061166]
[11]
Fouladvand, M.A.; Jafari, S.M. Prevalence of antibodies to Toxoplasma gondii in pregnant women of Bushehr. ISMJ, 2001, 3(2), 113-116.
[12]
Alcock, J.; White, P.; Zhao, F.; Scott, P.; Broadley, M.; Hart, D. Selenium biofortification of high-yielding winter wheat [Triticum aestivum L.] by liquid or granular Se fertilisation. Plant Soil, 2010, 332(1-2), 5-18.
[http://dx.doi.org/10.1007/s11104-009-0234-4]
[13]
Rotruck, J.T.; Pope, A.L.; Ganther, H.E.; Swanson, A.B.; Hafeman, D.G.; Hoekstra, W.G. Selenium: biochemical role as a component of glutathione peroxidase. Science, 1973, 179(4073), 588-590.
[http://dx.doi.org/10.1126/science.179.4073.588] [PMID: 4686466]
[14]
Agay, D.; Sandre, C.; Ducros, V.; Faure, H.; Cruz, C.; Alonso, A.; Roussel, A.M.; Chancerelle, Y. Optimization of selenium status by a single intraperitoneal injection of Se in Se-deficient rat: possible application to burned patient treatment. Free Radic. Biol. Med., 2005, 39(6), 762-768.
[http://dx.doi.org/10.1016/j.freeradbiomed.2005.04.024] [PMID: 16109306]
[15]
Kim, Y.S.; Combs, G.F., Jr Effects of dietary selenium and vitamin E on glutathione concentrations and glutathione S-transferase activities in chick liver and plasma. Nutr. Res., 1993, 13(4), 455-463.
[http://dx.doi.org/10.1016/S0271-5317(05)80710-9]
[16]
Nawrot, T.S.; Staessen, J.A.; Roels, H.A.; Den Hond, E.; Thijs, L.; Fagard, R.H.; Dominiczak, A.F.; Struijker-Boudier, H.A. Blood pressure and blood selenium: a cross-sectional and longitudinal population study. Eur. Heart J., 2007, 28(5), 628-633.
[http://dx.doi.org/10.1093/eurheartj/ehl479] [PMID: 17242009]
[17]
Kieliszek, M.; Błażejak, S. Current knowledge on the importance of selenium in food for living organisms: A review. Molecules, 2016, 21(5), 609.
[http://dx.doi.org/10.3390/molecules21050609] [PMID: 27171069]
[18]
Brigelius-Flohé, R.; Banning, A.; Kny, M.; Böl, G-F. Redox events in interleukin-1 signaling. Arch. Biochem. Biophys., 2004, 423(1), 66-73.
[http://dx.doi.org/10.1016/j.abb.2003.12.008] [PMID: 14989266]
[19]
Mercier, C.; Adjogble, K.D.; Däubener, W.; Delauw, M.F. Dense granules: are they key organelles to help understand the parasitophorous vacuole of all apicomplexa parasites? Int. J. Parasitol., 2005, 35(8), 829-849.
[http://dx.doi.org/10.1016/j.ijpara.2005.03.011] [PMID: 15978597]
[20]
Michelin, A.; Bittame, A.; Bordat, Y.; Travier, L.; Mercier, C.; Dubremetz, J.F.; Lebrun, M. GRA12, a Toxoplasma dense granule protein associated with the intravacuolar membranous nanotubular network. Int. J. Parasitol., 2009, 39(3), 299-306.
[http://dx.doi.org/10.1016/j.ijpara.2008.07.011] [PMID: 18840447]
[21]
Döşkaya, M.; Kalantari-Dehaghi, M.; Walsh, C.M.; Hiszczyńska-Sawicka, E.; Davies, D.H.; Felgner, P.L.; Larsen, L.S.; Lathrop, R.H.; Hatfield, G.W.; Schulz, J.R.; Gürüz, Y.; Jurnak, F. GRA1 protein vaccine confers better immune response compared to codon-optimized GRA1 DNA vaccine. Vaccine, 2007, 25(10), 1824-1837.
[http://dx.doi.org/10.1016/j.vaccine.2006.10.060] [PMID: 17234306]
[22]
Feng, P.; Park, J.; Lee, B-S.; Lee, S-H.; Bram, R.J.; Jung, J.U. Kaposi’s sarcoma-associated herpesvirus mitochondrial K7 protein targets a cellular calcium-modulating cyclophilin ligand to modulate intracellular calcium concentration and inhibit apoptosis. J. Virol., 2002, 76(22), 11491-11504.
[http://dx.doi.org/10.1128/JVI.76.22.11491-11504.2002] [PMID: 12388711]
[23]
Craver, M.P.J.; Knoll, L.J. Increased efficiency of homologous recombination in Toxoplasma gondii dense granule protein 3 demonstrates that GRA3 is not necessary in cell culture but does contribute to virulence. Mol. Biochem. Parasitol., 2007, 153(2), 149-157.
[http://dx.doi.org/10.1016/j.molbiopara.2007.02.013] [PMID: 17418907]
[24]
Halliwell, B. Free radicals and antioxidants: updating a personal view. Nutr. Rev., 2012, 70(5), 257-265.
[http://dx.doi.org/10.1111/j.1753-4887.2012.00476.x] [PMID: 22537212]
[25]
Pace, D.A.; McKnight, C.A.; Liu, J.; Jimenez, V.; Moreno, S.N. Calcium entry in Toxoplasma gondii and its enhancing effect of invasion-linked traits. J. Biol. Chem., 2014, 289(28), 19637-19647.
[http://dx.doi.org/10.1074/jbc.M114.565390] [PMID: 24867952]
[26]
Borges-Pereira, L.; Budu, A.; McKnight, C.A.; Moore, C.A.; Vella, S.A.; Hortua Triana, M.A.; Liu, J.; Garcia, C.R.; Pace, D.A.; Moreno, S.N. Calcium signaling throughout the toxoplasma gondii lytic cycle a study using genetically encoded calcium indicators. J. Biol. Chem., 2015, 290(45), 26914-26926.
[http://dx.doi.org/10.1074/jbc.M115.652511] [PMID: 26374900]
[27]
Jalalizadegan, B; Ghaffarifar, F; Fallah, S The Effect of Vitamins C and E on Nitric Oxide Elevation to Enhance Killing of Phagocytised Tachizoites of Toxoplasma gondii in BALB/c Mice. JSUMS, 2019, 26(4), 514-525.
[28]
Avery, J.C.; Hoffmann, P.R. Selenium, selenoproteins, and immunity. Nutrients, 2018, 10(9), 1203.
[http://dx.doi.org/10.3390/nu10091203] [PMID: 30200430]
[29]
Kurutas, E.B. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutr. J., 2016, 15(1), 71.
[http://dx.doi.org/10.1186/s12937-016-0186-5] [PMID: 27456681]
[30]
Johnson, S.M.; Murphy, R.C.; Geiger, J.A.; DeRocher, A.E.; Zhang, Z.; Ojo, K.K.; Larson, E.T.; Perera, B.G.; Dale, E.J.; He, P.; Reid, M.C.; Fox, A.M.; Mueller, N.R.; Merritt, E.A.; Fan, E.; Parsons, M.; Van Voorhis, W.C.; Maly, D.J. Development of Toxoplasma gondii calcium-dependent protein kinase 1 (TgCDPK1) inhibitors with potent anti-toxoplasma activity. J. Med. Chem., 2012, 55(5), 2416-2426.
[http://dx.doi.org/10.1021/jm201713h] [PMID: 22320388]
[31]
Fazli, M.S.; Vella, S.A.; Moreno, S.N.; Quinn, S. Computational motility tracking of calcium dynamics in Toxoplasma gondii. arXiv preprint arXiv:170801871., 2017.
[32]
Karaman, U.; Celik, T.; Kiran, T.R.; Colak, C.; Daldal, N.U. Malondialdehyde, glutathione, and nitric oxide levels in Toxoplasma gondii seropositive patients. Korean J. Parasitol., 2008, 46(4), 293-295.
[http://dx.doi.org/10.3347/kjp.2008.46.4.293] [PMID: 19127340]
[33]
Rodriguez-Cortes, A.; Martori, C.; Martinez-Florez, A.; Clop, A.; Amills, M.; Kubejko, J.; Llull, J.; Nadal, J.M.; Alberola, J. Canine Leishmaniasis Progression is Associated with Vitamin D Deficiency. Sci. Rep., 2017, 7(1), 3346.
[http://dx.doi.org/10.1038/s41598-017-03662-4] [PMID: 28611427]
[34]
Ghaffarifar, F.; Abdolah Pour, M.; Sharifi, Z.; Dalimi Asl, A.; Al-Kawaz, E. The effect of vitamin d3 alone and mixed with ifn-γ on tachyzoites of toxoplasma gondii (rh strain) proliferation and nitric oxide (no) production in infected macrophages of balb/c mice. Iran J Parasitol, 2010, 5(3), 48-56.
[PMID: 22347255]
[35]
Rajapakse, R.; Uring-Lambert, B.; Andarawewa, K.L.; Rajapakse, R.P.; Abou-Bacar, A.; Marcellin, L.; Candolfi, E. 1,25(OH)2D3 inhibits in vitro and in vivo intracellular growth of apicomplexan parasite Toxoplasma gondii. J. Steroid Biochem. Mol. Biol., 2007, 103(3-5), 811-814.
[http://dx.doi.org/10.1016/j.jsbmb.2006.12.058] [PMID: 17270431]
[36]
Genuardi, J.; Eskew, M.; Zeligs, B.; Bellanti, J. The effects of vitamin E and selenium on the nitric oxide production of macrophages. Pediatr. Res., 1999, 45(S5), 771.
[http://dx.doi.org/10.1203/00006450-199905010-00201]

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