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当代肿瘤药物靶点

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ISSN (Print): 1568-0096
ISSN (Online): 1873-5576

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Research Article

VS-5584通过调节PI3K / mTOR和MAPK途径诱导G1期阻滞抑制人骨肉瘤细胞的生长

卷 20, 期 8, 2020

页: [616 - 623] 页: 8

弟呕挨: 10.2174/1568009620666200414150353

价格: $65

摘要

背景：PI3K / mTOR信号通路的激活在人类骨肉瘤的进展中起关键作用。 研究证实，VS-5584是PI3K / mTOR途径的新型抑制剂，并显示出潜在的抗癌活性。 目的：探讨VS-5584对人骨肉瘤细胞生长的抗癌作用及其潜在机制。 方法：培养U2OS和MG-63人骨肉瘤细胞，通过CCK8分析，流式细胞术和Western blot研究VS-5584处理细胞的细胞毒性，细胞凋亡。 细胞迁移和管形成也用于检查抗癌潜力。 结果：结果表明，VS-5584处理通过调节p21，p27，Cyclin B1和Cdc2诱导G1期阻滞，剂量依赖性地抑制U2OS和MG-63细胞的生长。 进一步的研究表明，VS-5584处理可有效抑制PI3K / mTOR信号通路并触发MAPK磷酸化。 此外，VS-5584处理可显着抑制HUVEC的细胞迁移和管形成，然后下调HIF-1α和VEGF。 结论：我们的发现证实了VS-5584可能是一种有前途的抗癌药物，在人骨肉瘤的化学疗法和化学预防中具有潜在的应用前景。

关键词: VS-5584，骨肉瘤，G1期阻滞，PI3K / mTOR，MAPK，细胞毒性。

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